Acceleration of the Deamination of Cytosine through Photo-Crosslinking.
DNA manipulation
RNA editing
cytosine deamination
nucleobase editing
photo-crosslinking
Journal
Current issues in molecular biology
ISSN: 1467-3045
Titre abrégé: Curr Issues Mol Biol
Pays: Switzerland
ID NLM: 100931761
Informations de publication
Date de publication:
29 May 2023
29 May 2023
Historique:
received:
24
04
2023
revised:
18
05
2023
accepted:
26
05
2023
medline:
27
6
2023
pubmed:
27
6
2023
entrez:
27
6
2023
Statut:
epublish
Résumé
Herein, we report the major factor for deamination reaction rate acceleration, i.e., hydrophilicity, by using various 5-substituted target cytosines and by carrying out deamination at high temperatures. Through substitution of the groups at the 5'-position of the cytosine, the effect of hydrophilicity was understood. It was then used to compare the various modifications of the photo-cross-linkable moiety as well as the effect of the counter base of the cytosine to edit both DNA and RNA. Furthermore, we were able to achieve cytosine deamination at 37 °C with a half-life in the order of a few hours.
Identifiants
pubmed: 37367047
pii: cimb45060298
doi: 10.3390/cimb45060298
pmc: PMC10297672
doi:
Types de publication
Journal Article
Langues
eng
Pagination
4687-4700Subventions
Organisme : Japan Society for the Promotion of Science
ID : 17H03085
Organisme : Japan Society for the Promotion of Science
ID : 21H02076
Organisme : Japan Society for the Promotion of Science
ID : 19H04204
Organisme : Ministry of Education, Culture, Sports, Science and Technology
ID : 21H05876
Organisme : Asahi Glass (Japan)
Références
J Pharm Pharmacol. 1995 Apr;47(4):345-7
pubmed: 7791035
Nature. 1986 May 15-21;321(6067):209-13
pubmed: 2423876
Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1827-31
pubmed: 1542678
Arch Dis Child. 1998 Jul;79(1):22-7
pubmed: 9771247
Nucleic Acids Symp Ser. 1997;(37):203-4
pubmed: 9586070
Bioorg Med Chem Lett. 2017 Aug 15;27(16):3905-3908
pubmed: 28689971
J Biol Chem. 2015 Oct 30;290(44):26597-609
pubmed: 26354431
J Immunol. 2004 Jun 1;172(11):6513-8
pubmed: 15153462
J Cell Biochem. 2017 Nov;118(11):3586-3594
pubmed: 28460414
Chem Commun (Camb). 2010 Oct 28;46(40):7545-7
pubmed: 20848024
Enzyme Microb Technol. 2021 Sep;149:109851
pubmed: 34311888
Science. 2009 May 15;324(5929):929-30
pubmed: 19372393
Brain Res Mol Brain Res. 1995 Feb;28(2):281-95
pubmed: 7723627
Chembiochem. 2010 Aug 16;11(12):1661-4
pubmed: 20632434
FEBS J. 2010 Mar;277(6):1551-61
pubmed: 20148969
Commun Biol. 2021 Jul 16;4(1):882
pubmed: 34272468
Nat Biotechnol. 2013 Mar;31(3):233-9
pubmed: 23360965
Adv Genet. 2012;80:37-97
pubmed: 23084873
Nucleic Acids Res. 1988 Nov 25;16(22):10681-97
pubmed: 3060847
Nucleic Acids Res. 2000 Sep 15;28(18):E83
pubmed: 10982894
Biochemistry. 1996 Aug 6;35(31):10172-81
pubmed: 8756482
J Neurol Neurosurg Psychiatry. 2014 Mar;85(3):257-65
pubmed: 23772060
Org Biomol Chem. 2017 Jun 28;15(24):5109-5111
pubmed: 28470278
Nat Biotechnol. 2001 Aug;19(8):773-6
pubmed: 11479573
Ann Neurol. 1993 Sep;34(3):410-2
pubmed: 8395787
Nature. 2019 May;569(7756):433-437
pubmed: 30995674
Int J Dev Biol. 2013;57(6-8):629-37
pubmed: 24166445
Mol Biosyst. 2017 Jun 1;13(6):1152-1156
pubmed: 28453010
J Mol Biol. 1998 Nov 27;284(2):297-311
pubmed: 9813119
Ann Neurol. 2016 Feb;79(2):190-203
pubmed: 26506407
Am J Hum Genet. 1990 Nov;47(5):784-9
pubmed: 2171331
Int J Cancer. 1996 Jul 17;67(2):264-8
pubmed: 8760597
Biochemistry. 2001 Sep 25;40(38):11604-13
pubmed: 11560511
Org Lett. 2008 Aug 7;10(15):3227-30
pubmed: 18582065
Science. 2014 Nov 28;346(6213):1258096
pubmed: 25430774
Molecules. 2018 Apr 04;23(4):
pubmed: 29617316
Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3578-82
pubmed: 9520408
Nat Commun. 2021 Oct 6;12(1):5855
pubmed: 34615869