Effect of Gelatin-Based Hemostats on Fibroblasts and Relevant Growth Factors in Wound Healing.
cytokine
exposure
fibroblast
gelatin
hemostats
wound healing
Journal
Gels (Basel, Switzerland)
ISSN: 2310-2861
Titre abrégé: Gels
Pays: Switzerland
ID NLM: 101696925
Informations de publication
Date de publication:
20 Jun 2023
20 Jun 2023
Historique:
received:
08
06
2023
revised:
18
06
2023
accepted:
19
06
2023
medline:
27
6
2023
pubmed:
27
6
2023
entrez:
27
6
2023
Statut:
epublish
Résumé
Gelatin-based hemostats have been used in various surgical fields and showed advantageous effects on central aspects of wound healing when compared to cellulose-based hemostats. Nevertheless, the influence of gelatin-based hemostats on wound healing has not been fully explored yet. Hemostats were applied to fibroblast cell cultures for 5, 30, 60 min, 24 h, 7 and 14 days and measurements were taken at 3, 6, 12, 24 h and 7 or 14 days, respectively. Cell proliferation was quantified after different exposure times and a contraction assay was conducted to measure the extent of the extracellular matrix over time. We further assessed quantitative levels of vascular endothelial growth factor and basic fibroblast growth factor using enzyme-linked immunosorbent assay. Fibroblast counts decreased significantly at 7 and 14 days independent of the application duration (
Identifiants
pubmed: 37367174
pii: gels9060504
doi: 10.3390/gels9060504
pmc: PMC10298343
pii:
doi:
Types de publication
Journal Article
Langues
eng
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