Rapid visual detection of the allergen Dermatophagoides farinae in house dust by loop-mediated isothermal amplification.
Allergen
Dermatophagoides farinae
Loop-mediated isothermal amplification (LAMP)
Molecular detection
Journal
Environmental science and pollution research international
ISSN: 1614-7499
Titre abrégé: Environ Sci Pollut Res Int
Pays: Germany
ID NLM: 9441769
Informations de publication
Date de publication:
Jul 2023
Jul 2023
Historique:
received:
22
02
2023
accepted:
19
06
2023
medline:
24
7
2023
pubmed:
27
6
2023
entrez:
27
6
2023
Statut:
ppublish
Résumé
Dermatophagoides farinae is considered to be an important factor causing some allergic diseases, such as urticaria, allergic rhinitis, asthma, and other interrelated diseases. Avoiding exposure to allergens is the most effective way to reduce allergic reactions. In this study, we successfully established a loop-mediated isothermal amplification (LAMP) method for the detection of D. farinae DNA target internal transcribed spacer (ITS) and D. farinae 1 allergen (Der f 1) genes. The turbidity-monitoring system and visual fluorescent reagents were used to verify the test results of LAMP assay. Following optimization of the primers and reaction temperatures, the amplification sensitivity, specificity, and efficiency of the method for detecting D. farinae were assessed. There was no cross-reaction with other arthropod species that are commonly found in indoor environmental dust, such as Dermatophagoides pteronyssinus, Alophagoides ovatus, Periplaneta americana, Anopheles sinensis, and Musca domestica. Furthermore, the sensitivity of LAMP assay for detecting D. farinae DNA was 10 times greater than that of conventional PCR. The positive detection rate by the LAMP method was greater than the conventional PCR for both single D. farinae mites and D. farinae mites in indoor dust. A new type of LAMP method for D. farinae based on the Der f 1 and ITS genes was, therefore, successfully established. This study is the first time to detect the D. farinae allergen using LAMP assay. This assay could be useful as a model for the rapid detection of allergens produced by other house dust mites in the future.
Identifiants
pubmed: 37368209
doi: 10.1007/s11356-023-28398-9
pii: 10.1007/s11356-023-28398-9
doi:
Substances chimiques
Allergens
0
Dust
0
DNA
9007-49-2
Antigens, Dermatophagoides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
84674-84685Subventions
Organisme : grants from the Academic Aid Program for Top-notch Talents in Provincial Universities
ID : gxbjZD2020071
Organisme : Wuhu Key Research and Development Program of China
ID : 2021yf391
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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