Effects of Follicular Fluid on Physiological Characteristics and Differentiation of Fallopian Tube Epithelial Cells Implicating for Ovarian Cancer Pathogenesis.
cell injury
ciliogenesis
estrogen
fallopian tube
follicular fluid
organoid culture
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
15 Jun 2023
15 Jun 2023
Historique:
received:
18
05
2023
revised:
06
06
2023
accepted:
13
06
2023
medline:
29
6
2023
pubmed:
28
6
2023
entrez:
28
6
2023
Statut:
epublish
Résumé
The fallopian tube (FT) is an important reproductive organ in females. Ample evidence suggests that the distal end of FT is the original site of high-grade serous ovarian carcinoma (HGSC). FT may suffer from repeated injury and repair stimulated by follicular fluid (FF); however, this hypothesis has not been examined. In fact, the molecular mechanism of homeostasis, differentiation, and the transformation of fallopian tube epithelial cells (FTECs) resulting from the stimulation of FF are still enigmatic. In this study, we examined the effects of FF along with factors present in the FF on a variety of FTEC models, including primary cell culture, ALI (air-liquid interface) culture, and 3D organ spheroid culture. We found that FF plays a similar role to estrogen in promoting cell differentiation and organoid formation. Moreover, FF significantly promotes cell proliferation and induces cell injury and apoptosis in high concentrations. These observations may help us to investigate the mechanisms of the initiation of HGSC.
Identifiants
pubmed: 37373301
pii: ijms241210154
doi: 10.3390/ijms241210154
pmc: PMC10299324
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Science and Technology Planning Project of Guangdong Province
ID : 2020A1515110668, 2021A1515110916
Organisme : Foundation and Application foundation project support by Jiangmen City
ID : 2220002000296, 2021030103170007301
Organisme : Innovation team program supported by Guangdong Province
ID : 2020KCXTD038
Organisme : Guangdong Province Key Program of Discipline
ID : 2021ZDJS091
Organisme : National Natural Science Foundation of China
ID : 8194100101
Organisme : Special Project on Key Fields of Universities in Guangdong Province
ID : 2021ZDZX2046
Organisme : Science and Technology Planning Project of Guangdong Province
ID : 2021B1212040016
Références
Dev Growth Differ. 2007 Jan;49(1):61-6
pubmed: 17227345
Nat Commun. 2015 Dec 08;6:8989
pubmed: 26643275
Gynecol Oncol. 2014 Feb;132(2):322-7
pubmed: 24355484
Hum Reprod. 2013 Jan;28(1):224-9
pubmed: 23019297
Fertil Steril. 2015 Feb;103(2):303-16
pubmed: 25497448
Fertil Steril. 2003 Apr;79(4):829-43
pubmed: 12749418
Biology (Basel). 2020 May 17;9(5):
pubmed: 32429601
Cancer Res. 2021 Nov 15;81(22):5706-5719
pubmed: 34561272
J Pathol. 2017 May;242(1):3-6
pubmed: 28054715
Hum Reprod Update. 2006 Jul-Aug;12(4):363-72
pubmed: 16565155
Gynecol Oncol. 2015 Feb;136(2):348-54
pubmed: 25527363
J Assist Reprod Genet. 2018 May;35(5):735-751
pubmed: 29497954
Cells. 2019 Aug 19;8(8):
pubmed: 31430961
Reprod Biol Endocrinol. 2014 Jul 06;12:60
pubmed: 24997727
PLoS One. 2017 Feb 23;12(2):e0172192
pubmed: 28231273
Nat Rev Mol Cell Biol. 2015 Oct;16(10):625-38
pubmed: 26350076
Carcinogenesis. 2015 Nov;36(11):1419-28
pubmed: 26363031
Reprod Biomed Online. 2019 Jan;38(1):30-37
pubmed: 30527851
Biomolecules. 2020 Sep 03;10(9):
pubmed: 32899226
J Vis Exp. 2011 May 09;(51):
pubmed: 21610668
Cancers (Basel). 2018 Jul 30;10(8):
pubmed: 30061485
Theriogenology. 2011 Sep 15;76(5):900-10
pubmed: 21719086
Int J Dev Biol. 2012;56(10-12):819-31
pubmed: 23417404
J Anim Sci Biotechnol. 2019 Dec 10;10:94
pubmed: 31827787
Exp Biol Med (Maywood). 2014 Jan;239(1):24-32
pubmed: 24186266
Endocr Relat Cancer. 2011 Sep 20;18(5):627-42
pubmed: 21813729
Cancers (Basel). 2020 Apr 27;12(5):
pubmed: 32349388
Reprod Biomed Online. 2006 Apr;12(4):500-6
pubmed: 16740225
JBRA Assist Reprod. 2016 Mar 01;20(1):38-40
pubmed: 27203305
Biol Reprod. 2010 Jun;82(6):1021-9
pubmed: 20164441
Nat Commun. 2017 Oct 23;8(1):1093
pubmed: 29061967