Mechanistic Insights into the Antibiofilm Mode of Action of Ellagic Acid.
E. coli
WrbA
antibiofilm
ellagic acid
molecular dynamics
polyphenols
Journal
Pharmaceutics
ISSN: 1999-4923
Titre abrégé: Pharmaceutics
Pays: Switzerland
ID NLM: 101534003
Informations de publication
Date de publication:
17 Jun 2023
17 Jun 2023
Historique:
received:
11
05
2023
revised:
09
06
2023
accepted:
15
06
2023
medline:
28
6
2023
pubmed:
28
6
2023
entrez:
28
6
2023
Statut:
epublish
Résumé
Bacterial biofilm is a major contributor to the persistence of infection and the limited efficacy of antibiotics. Antibiofilm molecules that interfere with the biofilm lifestyle offer a valuable tool in fighting bacterial pathogens. Ellagic acid (EA) is a natural polyphenol that has shown attractive antibiofilm properties. However, its precise antibiofilm mode of action remains unknown. Experimental evidence links the NADH:quinone oxidoreductase enzyme WrbA to biofilm formation, stress response, and pathogen virulence. Moreover, WrbA has demonstrated interactions with antibiofilm molecules, suggesting its role in redox and biofilm modulation. This work aims to provide mechanistic insights into the antibiofilm mode of action of EA utilizing computational studies, biophysical measurements, enzyme inhibition studies on WrbA, and biofilm and reactive oxygen species assays exploiting a WrbA-deprived mutant strain of
Identifiants
pubmed: 37376205
pii: pharmaceutics15061757
doi: 10.3390/pharmaceutics15061757
pmc: PMC10302398
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
Appl Microbiol Biotechnol. 2010 Dec;88(6):1343-54
pubmed: 20803137
Biochem J. 2016 Mar 1;473(5):549-58
pubmed: 26621870
Microbiology (Reading). 2008 Jan;154(Pt 1):148-166
pubmed: 18174134
Biofouling. 2010 Aug;26(6):739-52
pubmed: 20711895
World J Microbiol Biotechnol. 2022 Oct 14;38(12):236
pubmed: 36229744
J Med Chem. 2006 Oct 19;49(21):6177-96
pubmed: 17034125
Microbiology (Reading). 2022 Jul;168(7):
pubmed: 35829699
Appl Environ Microbiol. 2022 Feb 8;88(3):e0199421
pubmed: 34851721
Front Microbiol. 2018 Nov 20;9:2804
pubmed: 30515144
Nat Prod Rep. 2020 Nov 1;37(11):1454-1477
pubmed: 32608431
Curr Pharm Des. 2020;26(24):2807-2816
pubmed: 32048958
PLoS Pathog. 2015 Sep 01;11(9):e1005147
pubmed: 26325183
Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5796-800
pubmed: 8516330
Environ Microbiol. 2012 Jul;14(7):1753-61
pubmed: 22176949
J Phys Chem B. 2016 Jun 9;120(22):4867-77
pubmed: 27183467
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
Biofouling. 2013 Sep;29(8):929-37
pubmed: 23906229
Assay Drug Dev Technol. 2011 Aug;9(4):342-53
pubmed: 21812660
Antioxidants (Basel). 2021 Jun 06;10(6):
pubmed: 34204135
Toxins (Basel). 2022 Aug 31;14(9):
pubmed: 36136541
PLoS One. 2015 Jul 01;10(7):e0131519
pubmed: 26132116
Nat Commun. 2019 Sep 6;10(1):4071
pubmed: 31492833
J Bacteriol. 2004 Nov;186(21):7186-95
pubmed: 15489429
Int J Mol Sci. 2022 Nov 08;23(22):
pubmed: 36430196
DNA Res. 2005;12(5):291-9
pubmed: 16769691
J Proteome Res. 2008 Nov;7(11):4659-69
pubmed: 18826300
Expert Opin Drug Discov. 2015 May;10(5):449-61
pubmed: 25835573
Bioorg Med Chem Lett. 2009 Nov 15;19(22):6353-7
pubmed: 19804970
J Bacteriol. 2005 Mar;187(5):1591-603
pubmed: 15716429
J Bacteriol. 2011 May;193(10):2587-97
pubmed: 21421764
Oxid Med Cell Longev. 2022 Feb 21;2022:3848084
pubmed: 35237379
Nat Rev Microbiol. 2016 Aug 11;14(9):563-75
pubmed: 27510863
J Biol Chem. 1998 Aug 14;273(33):20960-6
pubmed: 9694845
Biofouling. 2017 Mar;33(3):235-251
pubmed: 28270055
Langmuir. 2006 Feb 28;22(5):2378-83
pubmed: 16489832
Protein Sci. 2007 Oct;16(10):2301-5
pubmed: 17893367
J Med Microbiol. 2010 Apr;59(Pt 4):496-498
pubmed: 19959627
Future Microbiol. 2017 Jan;12:15-27
pubmed: 27922746
Can J Microbiol. 2003 Jul;49(7):443-9
pubmed: 14569285
J Bacteriol. 2006 May;188(10):3498-506
pubmed: 16672604