Heterologous Expression of Codon-Optimized Azurin Transferred by Magnetofection Method in MCF-10A Cells.
Azurin
Codon optimization
Gene cloning
MCF-10A
Magnetofection
Journal
Molecular biotechnology
ISSN: 1559-0305
Titre abrégé: Mol Biotechnol
Pays: Switzerland
ID NLM: 9423533
Informations de publication
Date de publication:
28 Jun 2023
28 Jun 2023
Historique:
received:
08
03
2023
accepted:
15
06
2023
medline:
28
6
2023
pubmed:
28
6
2023
entrez:
28
6
2023
Statut:
aheadofprint
Résumé
Transfection efficiency of the immortalized human breast epithelial cell line MCF-10A remains an issue that needs to be resolved. In this study, it was aimed to deliver a recombinant DNA (pCMV-Azu-GFP) to the MCF-10A cells by the magnetofection method using magnetic nanoparticles (MNPs) and a simple magnet to accelerate the DNA delivery. Surface positively modified silica-coated iron oxide MNPs (MSNP-NH
Identifiants
pubmed: 37378861
doi: 10.1007/s12033-023-00798-9
pii: 10.1007/s12033-023-00798-9
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Türkiye Bilimsel ve Teknolojik Araştırma Kurumu
ID : 118Z150
Organisme : Yükseköğretim Kurulu
ID : 100/2000
Organisme : Yükseköğretim Kurulu
ID : 100/2000
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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