Protocol for in vitro assessment of human monocyte transendothelial migration using a high-throughput live cell imaging system.
Cell Culture
Cell-based Assays
Immunology
Microscopy
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
27 Jun 2023
27 Jun 2023
Historique:
received:
10
01
2023
revised:
08
04
2023
accepted:
25
05
2023
medline:
28
6
2023
pubmed:
28
6
2023
entrez:
28
6
2023
Statut:
aheadofprint
Résumé
In vitro modeling of the different steps of immune cell recruitment is essential to decipher the role of endothelial cells in this process. Here, we present a protocol for the assessment of human monocyte transendothelial migration using a live cell imaging system. We describe steps for culture of fluorescent monocytic THP-1 cells and chemotaxis plate preparation with HUVEC monolayers. We then detail real-time analysis using the IncuCyte® S3 live-cell imaging system, image analysis, and assessment of transendothelial migration rates. For complete details on the use and execution of this protocol, please refer to Ladaigue et al.
Identifiants
pubmed: 37379221
pii: S2666-1667(23)00355-6
doi: 10.1016/j.xpro.2023.102388
pmc: PMC10331580
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102388Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
Références
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