Inhibition of chitin deacetylases to attenuate plant fungal diseases.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
29 06 2023
29 06 2023
Historique:
received:
18
11
2022
accepted:
20
06
2023
medline:
3
7
2023
pubmed:
30
6
2023
entrez:
29
6
2023
Statut:
epublish
Résumé
Phytopathogenic fungi secrete chitin deacetylase (CDA) to escape the host's immunological defense during infection. Here, we showed that the deacetylation activity of CDA toward chitin is essential for fungal virulence. Five crystal structures of two representative and phylogenetically distant phytopathogenic fungal CDAs, VdPDA1 from Verticillium dahliae and Pst_13661 from Puccinia striiformis f. sp. tritici, were obtained in ligand-free and inhibitor-bound forms. These structures suggested that both CDAs have an identical substrate-binding pocket and an Asp-His-His triad for coordinating a transition metal ion. Based on the structural identities, four compounds with a benzohydroxamic acid (BHA) moiety were obtained as phytopathogenic fungal CDA inhibitors. BHA exhibited high effectiveness in attenuating fungal diseases in wheat, soybean, and cotton. Our findings revealed that phytopathogenic fungal CDAs share common structural features, and provided BHA as a lead compound for the design of CDA inhibitors aimed at attenuating crop fungal diseases.
Identifiants
pubmed: 37385996
doi: 10.1038/s41467-023-39562-7
pii: 10.1038/s41467-023-39562-7
pmc: PMC10310857
doi:
Substances chimiques
chitin deacetylase
EC 3.5.1.41
Amidohydrolases
EC 3.5.-
Chitin
1398-61-4
Cladribine
47M74X9YT5
Cytidine Deaminase
EC 3.5.4.5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3857Informations de copyright
© 2023. The Author(s).
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