Considerations for reproducible omics in aging research.
Journal
Nature aging
ISSN: 2662-8465
Titre abrégé: Nat Aging
Pays: United States
ID NLM: 101773306
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
received:
30
01
2023
accepted:
01
06
2023
pmc-release:
01
02
2024
medline:
18
8
2023
pubmed:
30
6
2023
entrez:
29
6
2023
Statut:
ppublish
Résumé
Technical advancements over the past two decades have enabled the measurement of the panoply of molecules of cells and tissues including transcriptomes, epigenomes, metabolomes and proteomes at unprecedented resolution. Unbiased profiling of these molecular landscapes in the context of aging can reveal important details about mechanisms underlying age-related functional decline and age-related diseases. However, the high-throughput nature of these experiments creates unique analytical and design demands for robustness and reproducibility. In addition, 'omic' experiments are generally onerous, making it crucial to effectively design them to eliminate as many spurious sources of variation as possible as well as account for any biological or technical parameter that may influence such measures. In this Perspective, we provide general guidelines on best practices in the design and analysis of omic experiments in aging research from experimental design to data analysis and considerations for long-term reproducibility and validation of such studies.
Identifiants
pubmed: 37386258
doi: 10.1038/s43587-023-00448-4
pii: 10.1038/s43587-023-00448-4
pmc: PMC10527412
mid: NIHMS1913581
doi:
Substances chimiques
Proteome
0
Types de publication
Journal Article
Review
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
921-930Subventions
Organisme : NIA NIH HHS
ID : R01 AG076433
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM142395
Pays : United States
Informations de copyright
© 2023. Springer Nature America, Inc.
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