Gold nanostructures modified carbon-based electrode enhanced with methylene blue for point-of-care COVID-19 tests using isothermal amplification.
COVID-19
Electrochemical sensing
Loop-mediated isothermal amplification
SARS-CoV-2
coronavirus
Journal
Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R
Informations de publication
Date de publication:
01 Dec 2023
01 Dec 2023
Historique:
received:
11
07
2022
revised:
20
04
2023
accepted:
19
06
2023
medline:
2
8
2023
pubmed:
1
7
2023
entrez:
30
6
2023
Statut:
ppublish
Résumé
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) envelope (E) and RNA-dependent RNA polymerase (RdRP) genes were detected via electrochemical measurements using a screen-printed carbon electrode (SPCE) (3-electrode system) coupled with a battery-operated thin-film heater based on the loop-mediated isothermal amplification (LAMP) technique. The working electrodes of the SPCE sensor were decorated with synthesized gold nanostars (AuNSs) to obtain a large surface area and improve sensitivity. The LAMP assay was enhanced using a real-time amplification reaction system to detect the optimal target genes (E and RdRP) of SARS-CoV-2. The optimized LAMP assay was performed with diluted concentrations (from 0 to 10
Identifiants
pubmed: 37390671
pii: S0039-9140(23)00592-1
doi: 10.1016/j.talanta.2023.124841
pmc: PMC10290770
pii:
doi:
Substances chimiques
Methylene Blue
T42P99266K
DNA
9007-49-2
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
124841Informations de copyright
Copyright © 2023. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.