Comprehensive chemical profiling of two Dendrobium species and identification of anti-hepatoma active constituents from Dendrobium chrysotoxum by network pharmacology.
Biomarkers
D. chrysotoxum
D. nobile
Hepatoma
Metabolomics
Network pharmacology
Journal
BMC complementary medicine and therapies
ISSN: 2662-7671
Titre abrégé: BMC Complement Med Ther
Pays: England
ID NLM: 101761232
Informations de publication
Date de publication:
01 Jul 2023
01 Jul 2023
Historique:
received:
17
04
2023
accepted:
20
06
2023
medline:
3
7
2023
pubmed:
2
7
2023
entrez:
1
7
2023
Statut:
epublish
Résumé
Dendrobium nobile and Dendrobium chrysotoxum are important species of the genus Dendrobium and have great economic and medicinal value. However, the medicinal properties of these two plants remain poorly understood. This study aimed to investigate the medical properties of D. nobile and D. chrysotoxum by conducting a comprehensive chemical profiling of the two plants. Additionally, active compounds and predictive targets for anti-hepatoma activity in D. chrysotoxum extracts were identified using Network Pharmacology. Chemical profiling showed that altogether 65 phytochemicals were identified from D. nobile and D. chrysotoxum, with major classes as alkaloids, terpenoids, flavonoids, bibenzyls and phenanthrenes. About 18 compounds were identified as the important differential metabolites in D. nobile and D. chrysotoxum. Furtherly, CCK-8 results showed that the extracts of stems and leaves of D. nobile and D. chrysotoxum could inhibit the growth of Huh-7 cells, and the anti-hepatoma activity of extracts were dose-dependent. Among the extracts, the extract of D. chrysotoxum showed significant anti-hepatoma activity. In order to find the potential mechanism of anti-hepatoma activity of D. chrysotoxum, five key compounds and nine key targets were obtained through constructing and analyzing the compound-target-pathway network. The five key compounds were chrysotobibenzyl, chrysotoxin, moscatilin, gigantol and chrysotoxene. Nine key targets, including GAPDH, EGFR, ESR1, HRAS, SRC, CCND1, HIF1A, ERBB2 and MTOR, could be considered as the core targets of the anti-hepatoma activity of D. chrysotoxum. In this study, the chemical composition difference and anti-hepatoma activity of stems and leaves of D. nobile and D. chrysotoxum were compared, and the potential anti-hepatoma mechanism of D. chrysotoxum was revealed in a multi-target and multi-pathway manner.
Sections du résumé
BACKGROUND
BACKGROUND
Dendrobium nobile and Dendrobium chrysotoxum are important species of the genus Dendrobium and have great economic and medicinal value. However, the medicinal properties of these two plants remain poorly understood. This study aimed to investigate the medical properties of D. nobile and D. chrysotoxum by conducting a comprehensive chemical profiling of the two plants. Additionally, active compounds and predictive targets for anti-hepatoma activity in D. chrysotoxum extracts were identified using Network Pharmacology.
RESULTS
RESULTS
Chemical profiling showed that altogether 65 phytochemicals were identified from D. nobile and D. chrysotoxum, with major classes as alkaloids, terpenoids, flavonoids, bibenzyls and phenanthrenes. About 18 compounds were identified as the important differential metabolites in D. nobile and D. chrysotoxum. Furtherly, CCK-8 results showed that the extracts of stems and leaves of D. nobile and D. chrysotoxum could inhibit the growth of Huh-7 cells, and the anti-hepatoma activity of extracts were dose-dependent. Among the extracts, the extract of D. chrysotoxum showed significant anti-hepatoma activity. In order to find the potential mechanism of anti-hepatoma activity of D. chrysotoxum, five key compounds and nine key targets were obtained through constructing and analyzing the compound-target-pathway network. The five key compounds were chrysotobibenzyl, chrysotoxin, moscatilin, gigantol and chrysotoxene. Nine key targets, including GAPDH, EGFR, ESR1, HRAS, SRC, CCND1, HIF1A, ERBB2 and MTOR, could be considered as the core targets of the anti-hepatoma activity of D. chrysotoxum.
CONCLUSIONS
CONCLUSIONS
In this study, the chemical composition difference and anti-hepatoma activity of stems and leaves of D. nobile and D. chrysotoxum were compared, and the potential anti-hepatoma mechanism of D. chrysotoxum was revealed in a multi-target and multi-pathway manner.
Identifiants
pubmed: 37393306
doi: 10.1186/s12906-023-04048-y
pii: 10.1186/s12906-023-04048-y
pmc: PMC10314590
doi:
Substances chimiques
Plant Extracts
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
217Subventions
Organisme : Basic public welfare research program of Zhejiang Province
ID : LGN22H280004
Organisme : The key project at central government level of the ability establishment of sustainable use for valuable Chinese medicine resources
ID : 2060302
Informations de copyright
© 2023. The Author(s).
Références
Burlacu E, Tanase C. Anticancer potential of natural bark products-a review. Plants (Basel). 2021;10(9):1895. https://doi.org/10.3390/plants10091895
doi: 10.3390/plants10091895
pubmed: 34579427
Efferth T, Oesch F. Repurposing of plant alkaloids for cancer therapy: Pharmacology and toxicology. Semin Cancer Biol. 2021;68:143–63. https://doi.org/10.1016/j.semcancer.2019.12.010
doi: 10.1016/j.semcancer.2019.12.010
pubmed: 31883912
Taha KF, Khalil M, Abubakr MS, Shawky E. Identifying cancer-related molecular targets of Nandina domestica Thunb. By network pharmacology-based analysis in combination with chemical profiling and molecular docking studies. J Ethnopharmacol. 2020;249:112413. https://doi.org/10.1016/j.jep.2019.112413
doi: 10.1016/j.jep.2019.112413
pubmed: 31760157
Abotaleb M, Liskova A, Kubatka P, Büsselberg D. Therapeutic potential of plant phenolic acids in the treatment of cancer. Biomolecules. 2020;10(2):221. https://doi.org/10.3390/biom10020221
doi: 10.3390/biom10020221
pubmed: 32028623
pmcid: 7072661
Ma L, Zhang M, Zhao R, Wang D, Ma Y, Li A. Plant natural products: promising resources for cancer chemoprevention. Molecules. 2021;26(4):933. https://doi.org/10.3390/molecules26040933
doi: 10.3390/molecules26040933
pubmed: 33578780
pmcid: 7916513
Wei W, Rasul A, Sadiqa A, Sarfraz I, Hussain G, Nageen B, Liu X, Watanabe N, Selamoglu Z, Ali M, Li X, Li J. Curcumol: from plant roots to Cancer roots. Int J Biol Sci. 2019;15(8):1600–9. https://doi.org/10.7150/ijbs.34716
doi: 10.7150/ijbs.34716
pubmed: 31360103
pmcid: 6643219
Yang S, Zhang X, Cao Z, Zhao K, Wang S, Chen M, Hu X. Growth-promoting Sphingomonas paucimobilis ZJSH1 associated with Dendrobium officinale through phytohormone production and nitrogen fixation. Microb Biotechnol. 2014;7:611–20. https://doi.org/10.1111/1751-7915.12148
doi: 10.1111/1751-7915.12148
pubmed: 25142808
pmcid: 4265079
Li RQ, Li JF, Zhou ZY, Guo Y, Zhang TT, Tao FF, Hu XF, Liu WH. Antibacterial and antitumor activity of secondary metabolites of endophytic Fungi Ty5 from Dendrobium officinale. J Biobased Mater Bioenergy. 2018;12:184–93.
doi: 10.1166/jbmb.2018.1769
Teixeira da Silva JA, Ng TB. The medicinal and pharmaceutical importance of Dendrobium species. Appl Microbiol Biotechnol. 2017;101(6):2227–39. https://doi.org/10.1007/s00253-017-8169-9
doi: 10.1007/s00253-017-8169-9
pubmed: 28197691
Shen YC, Korkor NL, Xiao R, Pu Q, Hu M, Zhang SS, Kong DD, Zeng GH, Hu XF. Antagonistic activity of combined bacteria strains against southern blight pathogen of Dendrobium officinale. Biol Control. 2020;151:1049–9644.
doi: 10.1016/j.biocontrol.2020.104291
Committee NP. Pharmacopoeia of the People’s Republic of China, 2020 edn. Beijing: Chemical Industry Press.
Li Q, Liu C, Huang C, Wang M, Long T, Liu J, Shi J, Shi J, Li L, He Y, Xu DL. Transcriptome and metabonomics analysis revealed the molecular mechanism of differential metabolite production of Dendrobium nobile under different epiphytic patterns. Front Plant Sci. 2022;13:868472. https://doi.org/10.3389/fpls.2022.868472
doi: 10.3389/fpls.2022.868472
pubmed: 35656012
pmcid: 9152433
Nie X, Chen Y, Li W, Lu Y. Anti-aging properties of Dendrobium nobile Lindl: from molecular mechanisms to potential treatments. J Ethnopharmacol. 2020;257:112839. https://doi.org/10.1016/j.jep.2020.112839
doi: 10.1016/j.jep.2020.112839
pubmed: 32268205
He L, Su Q, Bai L, Li M, Liu J, Liu X, Zhang C, Jiang Z, He J, Shi J, Huang S, Guo L. Recent research progress on natural small molecule bibenzyls and its derivatives in Dendrobium species. Eur J Med Chem. 2020;204:112530. https://doi.org/10.1016/j.ejmech.2020.112530
doi: 10.1016/j.ejmech.2020.112530
pubmed: 32711292
Ng TB, Liu J, Wong JH, Ye X, Wing Sze SC, Tong Y, Zhang KY. Review of research on Dendrobium, a prized folk medicine. Appl Microbiol Biotechnol. 2012 Mar;93(5):1795–803. https://doi.org/10.1007/s00253-011-3829-7
Pei C, Mi CY, Sun LH, Liu WH, Li O, Hu XF. Diversity of endophytic bacteria of Dendrobium officinale based on culture-dependent and culture-independent methods. Biotechnol Biotechnol Equip. 2017;31:112–9.
doi: 10.1080/13102818.2016.1254067
Yang CW, Chuang TH, Wu PL, Huang WH, Lee SJ. Anti-inflammatory effects of 7-methoxycryptopleurine and structure-activity relations of phenanthroindolizidines and phenanthroquinolizidines. Biochem Biophys Res Commun. 2007;354(4):942–8. https://doi.org/10.1016/j.bbrc.2007.01.065
doi: 10.1016/j.bbrc.2007.01.065
pubmed: 17274949
Wang YH. Traditional uses, chemical constituents, pharmacological activities, and toxicological effects of Dendrobium leaves: a review. J Ethnopharmacol. 2021;270:113851. https://doi.org/10.1016/j.jep.2021.113851
doi: 10.1016/j.jep.2021.113851
pubmed: 33485987
Cao H, Ji Y, Li S, Lu L, Tian M, Yang W, Li H. Extensive metabolic profiles of Leaves and stems from the Medicinal Plant Dendrobiumofficinale Kimura et Migo. Metabolites. 2019;9(10):215. https://doi.org/10.3390/metabo9100215
doi: 10.3390/metabo9100215
pubmed: 31590300
pmcid: 6835975
Zhang Y, Chen N, Ding Z, Gu Z, Zhang L, Shi G. Characterization and bioactivity analysis of Dendrobium officinale stem and leaf polysacchride. J Food Sci Biotechnol. 2017;36:959–65.
Zhou GF, Pang MX, Chen SH, Lv GY, Yan MQ. [Comparison on polysaccharide content and PMP-HPLC fingerprints of polysaccharide in stems and leaves of Dendrobium officinale]. Zhongguo Zhong Yao Za Zhi. 2014;39(5):795–802. Chinese.
pubmed: 25204167
Liu WJ, Jiang ZM, Chen Y, Xiao PT, Wang ZY, Huang TQ, Liu EH. Network pharmacology approach to elucidate possible action mechanisms of Sinomenii Caulis for treating osteoporosis. J Ethnopharmacol. 2020;15:257:112871. https://doi.org/10.1016/j.jep.2020.112871
doi: 10.1016/j.jep.2020.112871
NamNam HH, Kim JS, Lee J, Seo YH, Kim HS, Ryu SM, Choi G, Moon BC, Lee AY. Pharmacological effects of Agastache rugosa against gastritis using a network pharmacology approach. Biomolecules. 2020;10(9):1298. https://doi.org/10.3390/biom10091298
doi: 10.3390/biom10091298
Li S, Xue X, Yang X, Zhou S, Wang S, Meng J. A network pharmacology approach used to estimate the active ingredients of Moutan cortex charcoal and the potential targets in hemorrhagic diseases. Biol Pharm Bull. 2019;42(3):432–41. https://doi.org/10.1248/bpb.b18-00756
doi: 10.1248/bpb.b18-00756
pubmed: 30828075
Zhao J, Lv C, Wu Q, Zeng H, Guo X, Yang J, Tian S, Zhang W. Computational systems pharmacology reveals an antiplatelet and neuroprotective mechanism of Deng-Zhan-Xi-Xin injection in the treatment of ischemic stroke. Pharmacol Res. 2019;147:104365. https://doi.org/10.1016/j.phrs.2019.104365
doi: 10.1016/j.phrs.2019.104365
pubmed: 31348992
Lan Q, Liu C, Wu Z, Ni C, Li J, Huang C, Wang H, Wei G. Does the metabolome of wild-like Dendrobium officinale of different origins have regional differences? Molecules. 2022;27(20):7024. https://doi.org/10.3390/molecules27207024
doi: 10.3390/molecules27207024
pubmed: 36296615
pmcid: 9609934
Yu X, Wang Y, Tao S, Sun S. Geniposide plays anti-tumor effects by down-regulation of microRNA-224 in HepG2 and Huh7 cell lines. Exp Mol Pathol. 2020;112:104349. https://doi.org/10.1016/j.yexmp.2019.104349
doi: 10.1016/j.yexmp.2019.104349
pubmed: 31778668
Machon C, Catez F, Venezia ND, Vanhalle F, Guyot L, Vincent A, Garcia M, Roy B, Diaz JJ, Guitton J. Study of intracellular anabolism of 5-fluorouracil and incorporation in nucleic acids based on an LC-HRMS method. J Pharm Anal. 2021;11(1):77–87. https://doi.org/10.1016/j.jpha.2020.04.001
doi: 10.1016/j.jpha.2020.04.001
pubmed: 33717614
Xia J, Li XY, Lin M, Yu JN, Zeng ZD, Ye F, Hu GJ, Miu Q, He QL, Zhang XD, Liang ZS. Screening out biomarkers of Tetrastigma hemsleyanum for anti-cancer and anti-inflammatory based on spectrum-effect relationship coupled with UPLC-Q-TOF-MS. Molecules. 2023;28(7):3021. https://doi.org/10.3390/molecules28073021
doi: 10.3390/molecules28073021
pubmed: 37049789
pmcid: 10096277
Daina A, Michielin O, Zoete V. SwissTargetPrediction: updated data and new features for efficient prediction of protein targets of small molecules. Nucleic Acids Res. 2019;47(W1):W357–64. https://doi.org/10.1093/nar/gkz382
doi: 10.1093/nar/gkz382
pubmed: 31106366
pmcid: 6602486
Piñero J, Queralt-Rosinach N, Bravo À, Deu-Pons J, Bauer-Mehren A, Baron M, Sanz F, Furlong LI. DisGeNET: a discovery platform for the dynamical exploration of human diseases and their genes. Database (Oxford). 2015 Apr 15;2015:bav028. https://doi.org/10.1093/database/bav028
Stelzer G, Rosen N, Plaschkes I, Zimmerman S, Twik M, Fishilevich S, Stein TI, Nudel R, Lieder I, Mazor Y, Kaplan S, Dahary D, Warshawsky D, Guan-Golan Y, Kohn A, Rappaport N, Safran M, Lancet D. The GeneCards suite: from gene data mining to disease genome sequence analyses. Curr Protoc Bioinformatics. 2016;54:1. https://doi.org/10.1002/cpbi.5
doi: 10.1002/cpbi.5
Amberger JS, Bocchini CA, Scott AF, Hamosh A. OMIM.org: leveraging knowledge across phenotype-gene relationships. Nucleic Acids Res. 2019;47(D1):D1038–43. https://doi.org/10.1093/nar/gky1151
doi: 10.1093/nar/gky1151
pubmed: 30445645
UniProt Consortium. UniProt: the universal protein knowledgebase in 2021. Nucleic Acids Res. 2021;49(D1):D480–9. https://doi.org/10.1093/nar/gkaa1100
doi: 10.1093/nar/gkaa1100
Szklarczyk D, Morris JH, Cook H. The STRING database in 2017: quality-controlled protein-protein association networks, made broadly accessible. Nucleic Acids Res. 2017;45(D1):D362–8. https://doi.org/10.1093/nar/gkw937
doi: 10.1093/nar/gkw937
pubmed: 27924014
Huang DW, Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 2009;4(1):44–57. https://doi.org/10.1038/nprot.2008.211
doi: 10.1038/nprot.2008.211
pubmed: 19131956
Kanehisa M, Goto S. KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 2000 Jan 1;28(1):27–30. https://doi.org/10.1093/nar/28.1.27
Kanehisa M. Toward understanding the origin and evolution of cellular organisms. Protein Sci. 2019 Nov;28(11):1947–51. https://doi.org/10.1002/pro.3715
Kanehisa M, Furumichi M, Sato Y, Ishiguro-Watanabe M, Tanabe M. KEGG: integrating viruses and cellular organisms. Nucleic Acids Res. 2021;49(D1):D545–51. https://doi.org/10.1093/nar/gkaa970
doi: 10.1093/nar/gkaa970
pubmed: 33125081
Prasad R, Rana NK, Koch B. Dendrobium chrysanthum ethanolic extract induces apoptosis via p53 up-regulation in HeLa cells and inhibits tumor progression in mice. J Complement Integr Med. 2017;14(2). https://doi.org/10.1515/jcim-2016-0070
Wu Y, Jing R, Jiang L, Jiang Y, Kuang Q, Ye L, Yang L, Li Y, Li M. Combination use of protein-protein interaction network topological features improves the predictive scores of deleterious non-synonymous single-nucleotide polymorphisms. Amino Acids. 2014;46(8):2025–35. https://doi.org/10.1007/s00726-014-1760-9
doi: 10.1007/s00726-014-1760-9
pubmed: 24849655
Dennis G Jr, Sherman BT, Hosack DA, Yang J, Gao W, Lane HC, Lempicki R. A. DAVID: database for annotation, visualization, and Integrated Discovery. Genome biology;2003, 4(5), P3.
Dixon RA, Strack D. Phytochemistry meets genome analysis, and beyond. Phytochemistry. 2003;62(6):815–6. https://doi.org/10.1016/s0031-9422(02)00712-4
doi: 10.1016/s0031-9422(02)00712-4
pubmed: 12590109
Hu W, Zheng Y, Xia P, Liang Z. The research progresses and future prospects of Tetrastigma hemsleyanum Diels et gilg: a valuable chinese herbal medicine. J Ethnopharmacol. 2021;271:113836. https://doi.org/10.1016/j.jep.2021.113836
doi: 10.1016/j.jep.2021.113836
pubmed: 33465440
Hu W, Xia P, Liang Z. Molecular cloning and structural analysis of key enzymes in Tetrastigma hemsleyanum for resveratrol biosynthesis. Int J Biol Macromol. 2021;190:19–32. https://doi.org/10.1016/j.ijbiomac.2021.08.178
doi: 10.1016/j.ijbiomac.2021.08.178
pubmed: 34478792
Xu L, Cao M, Wang Q, Xu J, Liu C, Ullah N, Li J, Hou Z, Liang Z, Zhou W, Liu A. Insights into the plateau adaptation of Salvia castanea by comparative genomic and WGCNA analyses. J Adv Res. 2022;42:221–35. https://doi.org/10.1016/j.jare.2022.02.004
doi: 10.1016/j.jare.2022.02.004
pubmed: 36089521
pmcid: 9788944
Xiang Q, Hu S, Ligaba-Osena A, Yang J, Tong F, Guo W. Seasonal Variation in Transcriptomic profiling of Tetrastigma hemsleyanum fully developed tuberous roots enriches candidate genes in essential metabolic pathways and Phytohormone Signaling. Front Plant Sci. 2021;12:659645. https://doi.org/10.3389/fpls.2021.659645
doi: 10.3389/fpls.2021.659645
pubmed: 34305963
pmcid: 8300961
Ke L, Yu D, Zheng H, Xu Y, Wu Y, Jiao J, Wang X, Mei J, Cai F, Zhao Y, Sun J, Zhang X, Sun Y. Function deficiency of GhOMT1 causes anthocyanidins over-accumulation and diversifies fibre colours in cotton (Gossypium hirsutum). Plant Biotechnol J. 2022;20(8):1546–60. https://doi.org/10.1111/pbi.13832
doi: 10.1111/pbi.13832
pubmed: 35503731
pmcid: 9342615
Singh D, Chaudhuri PK. A review on phytochemical and pharmacological properties of Holy basil (Ocimum sanctum L). Ind Crops Prod. 2018;118:367–82.
doi: 10.1016/j.indcrop.2018.03.048
Xiao J, Bai W. Bioactive phytochemicals. Crit Rev Food Sci Nutr. 2019;59(6):827–9.
doi: 10.1080/10408398.2019.1601848
pubmed: 31070480
Fukushima A, Takahashi M, Nagasaki H, Aono Y, Kobayashi M, Kusano M, Saito K, Kobayashi N, Arita M. Development of RIKEN plant metabolome MetaDatabase. Plant Cell Physiol. 2022;63(3):433–40. https://doi.org/10.1093/pcp/pcab173
doi: 10.1093/pcp/pcab173
pubmed: 34918130
Zhou B, Xiao JF, Tuli L, Ressom HW. LC-MS-based metabolomics. Mol Biosyst. 2012;8(2):470–81. https://doi.org/10.1039/c1mb05350g
doi: 10.1039/c1mb05350g
pubmed: 22041788
Xia PG, Li QQ, Liang ZS, Zhang XM, Yan KJ. Spaceflight breeding could improve the volatile constituents of Andrographis paniculata. Ind Crops Prod. 2021;171:113967.
doi: 10.1016/j.indcrop.2021.113967
Mahrous EA, Farag MA. Two dimensional NMR spectroscopic approaches for exploring plant metabolome: a review. J Adv Res. 2015;6(1):3–15. https://doi.org/10.1016/j.jare.2014.10.003
doi: 10.1016/j.jare.2014.10.003
pubmed: 25685540
Chen XM, Xiao SY, Guo SX. [Comparison of chemical compositions between Dendrobium candidum and Dendrobium nobile]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2006 Aug;28(4):524–9. Chinese.
Lam Y, Ng TB, Yao RM. Evaluation of chemical constituents and important mechanism of pharmacological biology in dendrobium plants. Evid Based Complement Alternat Med. 2015;2015:841752. https://doi.org/10.1155/2015/841752
doi: 10.1155/2015/841752
pubmed: 25945114
pmcid: 4402476
Liu Y, Zhang JQ, Zhan R, Chen YG. Isopentenylated bibenzyls and phenolic compounds from Dendrobium chrysotoxum Lindl. Chem Biodivers. 2022;19(6):e202200259. https://doi.org/10.1002/cbdv.202200259
doi: 10.1002/cbdv.202200259
pubmed: 35510718
Zhang Y, Zhang GQ, Zhang D, Liu XD, Xu XY, Sun WH, Yu X, Zhu X, Wang ZW, Zhao X, Zhong WY, Chen H, Yin WL, Huang T, Niu SC, Liu ZJ. Chromosome-scale assembly of the Dendrobium chrysotoxum genome enhances the understanding of orchid evolution. Hortic Res. 2021;8(1):183. https://doi.org/10.1038/s41438-021-00621-z
doi: 10.1038/s41438-021-00621-z
pubmed: 34465765
pmcid: 8408244
Takamiya T, Wongsawad P, Sathapattayanon A, Tajima N, Suzuki S, Kitamura S, Shioda N, Handa T, Kitanaka S, Iijima H, Yukawa T. Molecular phylogenetics and character evolution of morphologically diverse groups, Dendrobium section Dendrobium and allies. AoB Plants. 2014;6:plu045. https://doi.org/10.1093/aobpla/plu045
doi: 10.1093/aobpla/plu045
pubmed: 25107672
pmcid: 4172198
Zheng S, Hu Y, Zhao R, Zhao T, Li H, Rao D, Chun Z. Quantitative assessment of secondary metabolites and cancer cell inhibiting activity by high performance liquid chromatography fingerprinting in Dendrobium nobile. J Chromatogr B Analyt Technol Biomed Life Sci. 2020;1140:122017. https://doi.org/10.1016/j.jchromb.2020.122017
doi: 10.1016/j.jchromb.2020.122017
pubmed: 32050157
Gong YQ, Fan Y, Wu DZ, Yang H, Hu ZB, Wang ZT. In vivo and in vitro evaluation of erianin, a novel anti-angiogenic agent. Eur J Cancer. 2004;40(10):1554–65. https://doi.org/10.1016/j.ejca.2004.01.041
doi: 10.1016/j.ejca.2004.01.041
pubmed: 15196540
Yang LC, Deng H, Yi Y, Zhang XM, Wang YZ, Lin JQ. [Identification of medical Dendrobium herbs by ISSR marker]. Zhong Yao Cai. 2010;33(12):1841–4. Chinese.
pubmed: 21548356
Hindson J. Lenvatinib plus EGFR inhibition for liver cancer. Nat Reviews Gastroenterology&Hepatology. 2021;18:675. https://doi.org/10.1038/s41575-021-00513-6
doi: 10.1038/s41575-021-00513-6
Yamada K, Kizawa R, Yoshida A, Koizumi R, Motohashi S, Shimoyama Y, Hannya Y, Yoshida S, Oikawa T, Shimoda M, Yoshida K. Extracellular PKCδ signals to epidermal growth factor receptor for tumor proliferation in liver cancer cells. Cancer Sci. 2022;113(7):2378–85. https://doi.org/10.1111/cas.15386
doi: 10.1111/cas.15386
pubmed: 35490382
pmcid: 9277411
Yang L, Peng F, Qin J, Zhou H, Wang B. Downregulation of microRNA-196a inhibits human liver cancer cell proliferation and invasion by targeting FOXO1. Oncol Rep. 2017;38:2148–54. https://doi.org/10.3892/or.2017.5873
doi: 10.3892/or.2017.5873
pubmed: 28791406
pmcid: 5652959
Wang Q, Yang X, Zhou X, Wu B, Zhu D, Jia W, Chu J, Wang J, Wu J, Kong L. MiR-3174 promotes proliferation and inhibits apoptosis by targeting FOXO1 in hepatocellular carcinoma. Biochem Biophys Res Commun. 2020;526:889–97. https://doi.org/10.1016/j.bbrc.2020.03.152
doi: 10.1016/j.bbrc.2020.03.152
pubmed: 32279994