Glucose-Responsive Fibrin Hydrogel-Based Multimodal Nucleic Acid Delivery System.
biomaterials
drug delivery
fibrin
glucose-responsive
microcapsules
microparticles
nucleic acids
wound healing
Journal
Advanced biology
ISSN: 2701-0198
Titre abrégé: Adv Biol (Weinh)
Pays: Germany
ID NLM: 101775319
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
27
04
2023
pubmed:
4
7
2023
medline:
4
7
2023
entrez:
4
7
2023
Statut:
ppublish
Résumé
Nucleic acid therapy has emerged as a potential alternative for promoting wound healing by gene expression modification. On the other hand, protecting the nucleic acid payload from degradation, efficient bioresponsive delivery and effective transfection into cells remain challenging. A glucose-responsive gene delivery system for treating diabetic wounds would be advantageous as it would be responsive to the underlying pathology giving a regulated payload delivery with fewer side effects. Herein a GOx-based glucose-responsive delivery system is designed based on fibrin-coated polymeric microcapsules (FCPMC) using the layer-by-layer (LbL) approach that simultaneously delivers two nucleic acids in diabetic wounds. The designed FCPMC displays an ability to effectively load many nucleic acids in polyplexes and release it over a prolonged period with no cytotoxic effects seen in in vitro studies. Furthermore, the developed system does not show any undesired effects in vivo. When applied to wounds in genetically diabetic db/db mice, the fabricated system on its own improves reepithelialization and angiogenesis while decreasing inflammation. Also, key proteins involved in the wound healing process, i.e., Actn2, MYBPC1, and desmin, are upregulated in the glucose-responsive fibrin hydrogel (GRFHG) treated group of animals. In conclusion, the fabricated hydrogel promotes wound healing. Furthermore, the system may be encapsulated with various therapeutic nucleic acids that aid wound healing.
Identifiants
pubmed: 37401646
doi: 10.1002/adbi.202300161
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300161Subventions
Organisme : Science Foundation Ireland
Pays : Ireland
Informations de copyright
© 2023 The Authors. Advanced Biology published by Wiley-VCH GmbH.
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