Trifluoroethanol and the behavior of a tardigrade desiccation-tolerance protein.
aggregation
desiccation tolerance
disorder
infrared spectroscopy
phase separation
tardigrades
trifluoroethanol
Journal
Protein science : a publication of the Protein Society
ISSN: 1469-896X
Titre abrégé: Protein Sci
Pays: United States
ID NLM: 9211750
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
revised:
26
06
2023
received:
11
05
2023
accepted:
27
06
2023
pmc-release:
01
08
2024
medline:
31
7
2023
pubmed:
4
7
2023
entrez:
4
7
2023
Statut:
ppublish
Résumé
The cosolvent 2,2,2-trifluoroethanol (TFE) is often used to mimic protein desiccation. We assessed the effects of TFE on cytosolic abundant heat soluble protein D (CAHS D) from tardigrades. CAHS D is a member of a unique protein class that is necessary and sufficient for tardigrades to survive desiccation. We find that the response of CAHS D to TFE depends on the concentration of both species. Dilute CAHS D remains soluble and, like most proteins exposed to TFE, gains α-helix. More concentrated solutions of CAHS D in TFE accumulate β-sheet, driving both gel formation and aggregation. At even higher TFE and CAHS D concentrations, samples phase separate without aggregation or increases in helix. Our observations show the importance of considering protein concentration when using TFE.
Identifiants
pubmed: 37401908
doi: 10.1002/pro.4716
pmc: PMC10357939
doi:
Substances chimiques
Trifluoroethanol
75-89-8
Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e4716Subventions
Organisme : NCI NIH HHS
ID : P30 CA016086
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM127291
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM127791
Pays : United States
Informations de copyright
© 2023 The Protein Society.
Références
Int J Biol Macromol. 2009 Mar 1;44(2):163-9
pubmed: 19100763
Proteomics. 2003 Aug;3(8):1418-24
pubmed: 12923766
Bioessays. 2017 Nov;39(11):
pubmed: 28901557
Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13407-12
pubmed: 9811813
Protein J. 2020 Aug;39(4):318-327
pubmed: 32656609
Biochim Biophys Acta. 2007 Sep;1767(9):1073-101
pubmed: 17692815
Protein Sci. 2022 Feb;31(2):396-406
pubmed: 34766407
Biochemistry (Mosc). 2006;71 Suppl 1:S77-82
pubmed: 16487073
Angew Chem Int Ed Engl. 2022 Jan 3;61(1):e202109961
pubmed: 34750927
Protein Sci. 2022 Dec;31(12):e4495
pubmed: 36335581
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Apr 15;1083:180-188
pubmed: 29549741
Proc Natl Acad Sci U S A. 2015 Jun 16;112(24):E3095-103
pubmed: 26038575
Planta. 2015 Aug;242(2):379-88
pubmed: 25809151
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
Biochemistry. 2019 Sep 17;58(37):3825-3833
pubmed: 31436413
Mol Cell. 2017 Mar 16;65(6):975-984.e5
pubmed: 28306513
Physiol Biochem Zool. 2014 Sep-Oct;87(5):640-51
pubmed: 25244376
Methods Mol Biol. 2012;895:387-404
pubmed: 22760329
Protein Sci. 2023 Aug;32(8):e4716
pubmed: 37401908
Biophys J. 2023 Jun 20;122(12):2500-2505
pubmed: 37149732
Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):18850-5
pubmed: 20947801
J Photochem Photobiol B. 2015 Mar;144:1-7
pubmed: 25662040
Protein Sci. 1994 Aug;3(8):1253-60
pubmed: 7987220
Biochemistry. 1998 Oct 13;37(41):14613-22
pubmed: 9772190
J Phys Chem B. 2018 Jul 19;122(28):7178-7184
pubmed: 29924608
Biophys J. 2012 Feb 22;102(4):897-906
pubmed: 22385861
Biochemistry. 2005 Aug 9;44(31):10669-80
pubmed: 16060675
F1000Res. 2017 Feb 7;6:113
pubmed: 28232867
J Phys Chem B. 2017 Sep 28;121(38):8935-8945
pubmed: 28851219
Matrix Biol. 2007 Mar;26(2):125-35
pubmed: 17129717
Protein Sci. 2019 May;28(5):941-951
pubmed: 30868674
J Am Chem Soc. 2002 Mar 6;124(9):1884-8
pubmed: 11866599
J Control Release. 2021 Aug 10;336:480-498
pubmed: 34214597
Plant Cell. 2007 May;19(5):1580-9
pubmed: 17526751
PLoS Biol. 2022 Sep 6;20(9):e3001780
pubmed: 36067153
PLoS One. 2012;7(8):e44209
pubmed: 22937162
Front Pharmacol. 2020 Oct 07;11:565969
pubmed: 33117164