A comparison of results from antihuman globulin-graded reactions with the monocyte monolayer assay.

antiglobulin antihuman globulin (AHG) extravascular/intravascular hemolysis monocyte index (MI) monocyte monolayer assay (MMA)

Journal

Immunohematology
ISSN: 0894-203X
Titre abrégé: Immunohematology
Pays: United States
ID NLM: 8806387

Informations de publication

Date de publication:
01 Jun 2023
Historique:
medline: 7 7 2023
pubmed: 5 7 2023
entrez: 5 7 2023
Statut: epublish

Résumé

Blood transfusions are a common medical treatment. Risks arise when compatible blood is not available. This study assesses the correlation between antibody reaction strength at the antihuman globulin (AHG) phase of testing and the antibody clinical significance as predicted using the monocyte monolayer assay (MMA). Multiple examples of anti-K donor plasma samples were selected to sensitize K+k+ red blood cells (RBCs). Reactivity was confirmed by testing the sensitized K+k+ RBCs at saline-AHG. Antibody titers were determined by serial dilution using neat plasma. Sixteen samples were selected for the study based on comparable graded reactions with neat plasma (1+, 2+, 3+, and 4+) and similar titration endpoints. Each sample was used to sensitize the same Kk donor and then tested by monocytes to evaluate the clinical significance using the MMA, an

Identifiants

pubmed: 37405849
pii: immunohematology-2023-013
doi: 10.21307/immunohematology-2023-013
doi:

Substances chimiques

Antibodies 0
Blood Group Antigens 0
Isoantibodies 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

77-81

Informations de copyright

© 2023 K. Bowman et al., published by Sciendo.

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Auteurs

K Bowman (K)

MLS(ASCP)SBBCM, Immunohematology Technologist III, LifeShare Blood Center, Shreveport, LA, USA.

L A Peña Marquez (LA)

BS, Lab Analyst, LifeShare Blood Center, Shreveport, LA, USA.

L Hawthorne (L)

MHS, MLS(ASCP)SBB, Immunohematology Technologist II, LifeShare Blood Center, Shreveport, LA, USA.

K Billingsley (K)

MSTM, MLS(ASCP)SBBCM Director, Scientific Services, LifeShare Blood Center, 8910 Linwood Avenue, Shreveport, LA 71106, USA.

S Kelham (S)

MLS(ASCP), Supervisor, Scientific Services, LifeShare Blood Center, Shreveport, LA, USA.

S Liang (S)

MLS(ASCP)SBBCM, Immunohematology Technologist II, LifeShare Blood Center, Shreveport, LA, USA.

M Kalvelage (M)

MSTM, MLS(ASCP)MBCM, SBBCM, Chief Operations Officer, LifeShare Blood Center, Shreveport, LA, USA.

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