Reactive astrocytes mediate TSPO overexpression in response to sustained CNTF exposure in the rat striatum.
CNTF
Endothelial cells
Fluorescence-activated cell sorting to radioligand-treated tissue
Lentiviral vectors - SPECT
Microglia
SOCS3 - astrocytes
TSPO
Journal
Molecular brain
ISSN: 1756-6606
Titre abrégé: Mol Brain
Pays: England
ID NLM: 101468876
Informations de publication
Date de publication:
05 07 2023
05 07 2023
Historique:
received:
12
01
2023
accepted:
01
06
2023
medline:
7
7
2023
pubmed:
6
7
2023
entrez:
5
7
2023
Statut:
epublish
Résumé
The 18 kDa translocator protein (TSPO) is a classical marker of neuroinflammation targeted for in vivo molecular imaging. Microglial cells were originally thought to be the only source of TSPO overexpression but astrocytes, neurons and endothelial cells can also up-regulate TSPO depending on the pathological context. This study aims to determine the cellular origin of TSPO overexpression in a simplified model of neuroinflammation and to identify the molecular pathways involved. This is essential to better interpret TSPO molecular imaging in preclinical and clinical settings. We used lentiviral vectors (LV) to overexpress the ciliary neurotrophic factor (CNTF) in the right striatum of 2-month-old Sprague Dawley rats. A LV encoding for β-Galactosidase (LV-LacZ) was used as control. One month later, TSPO expression was measured by single-photon emission computed tomography (SPECT) imaging using [
Identifiants
pubmed: 37408083
doi: 10.1186/s13041-023-01041-x
pii: 10.1186/s13041-023-01041-x
pmc: PMC10320938
doi:
Substances chimiques
Carrier Proteins
0
Ciliary Neurotrophic Factor
0
Tspo protein, rat
141440-82-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
57Informations de copyright
© 2023. The Author(s).
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