Rapid prototyping of a polymer MEMS droplet dispenser by laser-assisted 3D printing.
Biosensors
Electrical and electronic engineering
Nanofabrication and nanopatterning
Journal
Microsystems & nanoengineering
ISSN: 2055-7434
Titre abrégé: Microsyst Nanoeng
Pays: England
ID NLM: 101695458
Informations de publication
Date de publication:
2023
2023
Historique:
received:
03
05
2022
revised:
01
05
2023
accepted:
23
05
2023
medline:
6
7
2023
pubmed:
6
7
2023
entrez:
6
7
2023
Statut:
epublish
Résumé
In this work, we introduce a polymer version of a previously developed silicon MEMS drop deposition tool for surface functionalization that consists of a microcantilever integrating an open fluidic channel and a reservoir. The device is fabricated by laser stereolithography, which offers the advantages of low-cost and fast prototyping. Additionally, thanks to the ability to process multiple materials, a magnetic base is incorporated into the cantilever for convenient handling and attachment to the holder of a robotized stage used for spotting. Droplets with diameters ranging from ∼50 µm to ∼300 µm are printed upon direct contact of the cantilever tip with the surface to pattern. Liquid loading is achieved by fully immersing the cantilever into a reservoir drop, where a single load results in the deposition of more than 200 droplets. The influences of the size and shape of the cantilever tip and the reservoir on the printing outcome are studied. As a proof-of-concept of the biofunctionalization capability of this 3D printed droplet dispenser, microarrays of oligonucleotides and antibodies displaying high specificity and no cross-contamination are fabricated, and droplets are deposited at the tip of an optical fiber bundle.
Identifiants
pubmed: 37408536
doi: 10.1038/s41378-023-00559-3
pii: 559
pmc: PMC10318032
doi:
Types de publication
Journal Article
Langues
eng
Pagination
85Informations de copyright
© The Author(s) 2023.
Déclaration de conflit d'intérêts
Conflict of interestThe authors declare no competing interests.
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