Physicochemical Nature of SARS-CoV-2 Spike Protein Binding to Human Vimentin.
SARS-CoV-2
quartz crystal microbalance
single-molecule binding interactions
single-molecule force spectroscopy
vimentin
Journal
ACS applied materials & interfaces
ISSN: 1944-8252
Titre abrégé: ACS Appl Mater Interfaces
Pays: United States
ID NLM: 101504991
Informations de publication
Date de publication:
19 Jul 2023
19 Jul 2023
Historique:
medline:
21
7
2023
pubmed:
6
7
2023
entrez:
6
7
2023
Statut:
ppublish
Résumé
Vimentin, a protein that builds part of the cytoskeleton and is involved in many aspects of cellular function, was recently identified as a cell surface attachment site for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The present study investigated the physicochemical nature of the binding between the SARS-CoV-2 S1 glycoprotein receptor binding domain (S1 RBD) and human vimentin using atomic force microscopy and a quartz crystal microbalance. The molecular interactions of S1 RBD and vimentin proteins were quantified using vimentin monolayers attached to the cleaved mica or a gold microbalance sensor as well as in its native extracellular form present on the live cell surface. The presence of specific interactions between vimentin and S1 RBD was also confirmed using in silico studies. This work provides new evidence that cell-surface vimentin (CSV) functions as a site for SARS-CoV-2 virus attachment and is involved in the pathogenesis of Covid-19, providing a potential target for therapeutic countermeasures.
Identifiants
pubmed: 37413693
doi: 10.1021/acsami.3c03347
pmc: PMC10360031
doi:
Substances chimiques
spike protein, SARS-CoV-2
0
Spike Glycoprotein, Coronavirus
0
Vimentin
0
Angiotensin-Converting Enzyme 2
EC 3.4.17.23
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
34172-34180Références
Rev Med Virol. 2021 Sep;31(5):1-9
pubmed: 33368788
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W382-8
pubmed: 15980494
Int J Mol Sci. 2021 Jul 12;22(14):
pubmed: 34299089
Soft Matter. 2014 Mar 28;10(12):1924-30
pubmed: 24652035
Elife. 2020 Nov 09;9:
pubmed: 33164751
Biophys J. 2021 Mar 16;120(6):1097-1104
pubmed: 33253634
Nature. 2020 May;581(7807):215-220
pubmed: 32225176
Nat Commun. 2014 Jul 31;5:4463
pubmed: 25079911
Sci Adv. 2018 Aug 17;4(8):eaat1273
pubmed: 30128355
Bioconjug Chem. 2011 Jun 15;22(6):1239-48
pubmed: 21542606
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
J Biomed Sci. 2016 Jan 22;23:14
pubmed: 26801988
Nat Commun. 2020 Sep 11;11(1):4541
pubmed: 32917884
J Mol Biol. 2021 Jul 23;433(15):167058
pubmed: 34023401
Small. 2022 Feb;18(6):e2105640
pubmed: 34866333
Colloids Surf B Biointerfaces. 2016 Mar 1;139:123-31
pubmed: 26705826
Nanomaterials (Basel). 2021 Jan 08;11(1):
pubmed: 33435619
Nature. 2021 Aug;596(7873):583-589
pubmed: 34265844
Nat Commun. 2019 Oct 1;10(1):4460
pubmed: 31575869
Biophys J. 1995 Jun;68(6):2580-7
pubmed: 7647261
J Mol Biol. 2016 Feb 22;428(4):720-725
pubmed: 26410586
Biomacromolecules. 2022 Aug 8;23(8):3308-3317
pubmed: 35829774
Int J Mol Sci. 2020 Jun 30;21(13):
pubmed: 32630064
Structure. 2015 Jul 7;23(7):1350-61
pubmed: 26073602
Proc Natl Acad Sci U S A. 2020 May 26;117(21):11727-11734
pubmed: 32376634
Science. 1978 May 12;200(4342):618-27
pubmed: 347575
Nucleic Acids Res. 2018 Jul 2;46(W1):W363-W367
pubmed: 29860391
J Virol. 2014 May;88(10):5816-33
pubmed: 24623428
Proc Natl Acad Sci U S A. 2022 Feb 8;119(6):
pubmed: 35078919
Langmuir. 2015 Mar 17;31(10):2988-96
pubmed: 25692665
ACS Chem Biol. 2019 Mar 15;14(3):534-542
pubmed: 30735356
J Mol Biol. 2021 Jul 23;433(15):167051
pubmed: 33992693
Biosens Bioelectron. 2012 Jun-Jul;36(1):103-9
pubmed: 22541811
Nat Rev Mol Cell Biol. 2022 Jan;23(1):3-20
pubmed: 34611326
J Cell Sci. 2020 Nov 5;134(5):
pubmed: 33154171