Integrated multifunctional nanoplatform for fluorescence detection and inactivation of Staphylococcus aureus.
Cascaded signal amplification
Fluorescence detection
Inactivation
Integrated multifunctional nanoplatform
Staphylococcus aureus
Journal
Food chemistry
ISSN: 1873-7072
Titre abrégé: Food Chem
Pays: England
ID NLM: 7702639
Informations de publication
Date de publication:
01 Dec 2023
01 Dec 2023
Historique:
received:
11
03
2023
revised:
21
06
2023
accepted:
30
06
2023
medline:
31
7
2023
pubmed:
7
7
2023
entrez:
6
7
2023
Statut:
ppublish
Résumé
Foodborne illness caused by Staphylococcus aureus (S. aureus) has posed a significant threat to human health. Herein, an integrated multifunctional nanoplatform was developed for fluorescence detection and inactivation of S. aureus based on cascade signal amplification coupled with single strand DNA-template copper nanoparticles (ssDNA-Cu NPs). Benefiting from reasonable design, one-step cascade signal amplification was achieved through strand displacement amplification combined with rolling circle amplification, followed by in-situ generation of copper nanoparticles. S. aureus detection could be performed through naked eye observation and microplate reader measurement of the red fluorescence signal. The multifunctional nanoplatform had satisfactory specificity and sensitivity, achieving 5.2 CFU mL
Identifiants
pubmed: 37413833
pii: S0308-8146(23)01398-5
doi: 10.1016/j.foodchem.2023.136780
pii:
doi:
Substances chimiques
Copper
789U1901C5
DNA, Single-Stranded
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
136780Informations de copyright
Copyright © 2023. Published by Elsevier Ltd.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.