ACSL1 is a key regulator of inflammatory and macrophage foaming induced by short-term palmitate exposure or acute high-fat feeding.

Cellular physiology Immunology Physiology

Journal

iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038

Informations de publication

Date de publication:
21 Jul 2023
Historique:
received: 09 09 2022
revised: 29 04 2023
accepted: 12 06 2023
medline: 7 7 2023
pubmed: 7 7 2023
entrez: 7 7 2023
Statut: epublish

Résumé

Foamy and inflammatory macrophages play pathogenic roles in metabolic disorders. However, the mechanisms that promote foamy and inflammatory macrophage phenotypes under acute-high-fat feeding (AHFF) remain elusive. Herein, we investigated the role of acyl-CoA synthetase-1 (ACSL1) in favoring the foamy/inflammatory phenotype of monocytes/macrophages upon short-term exposure to palmitate or AHFF. Palmitate exposure induced a foamy/inflammatory phenotype in macrophages which was associated with increased ACSL1 expression. Inhibition/knockdown of ACSL1 in macrophages suppressed the foamy/inflammatory phenotype through the inhibition of the CD36-FABP4-p38-PPARδ signaling axis. ACSL1 inhibition/knockdown suppressed macrophage foaming/inflammation after palmitate stimulation by downregulating the FABP4 expression. Similar results were obtained using primary human monocytes. As expected, oral administration of ACSL1 inhibitor triacsin-C in mice before AHFF normalized the inflammatory/foamy phenotype of the circulatory monocytes by suppressing FABP4 expression. Our results reveal that targeting ACSL1 leads to the attenuation of the CD36-FABP4-p38-PPARδ signaling axis, providing a therapeutic strategy to prevent the AHFF-induced macrophage foaming and inflammation.

Identifiants

pubmed: 37416456
doi: 10.1016/j.isci.2023.107145
pii: S2589-0042(23)01222-1
pmc: PMC10320618
doi:

Types de publication

Journal Article

Langues

eng

Pagination

107145

Informations de copyright

© 2023 The Author(s).

Déclaration de conflit d'intérêts

The authors declare that they have no competing interests.

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Auteurs

Fatema Al-Rashed (F)

Immunology & Microbiology Department, Dasman Diabetes Institute, Kuwait City, Dasman 15462, Kuwait.

Dania Haddad (D)

Genetics and Bioinformatics Department, Dasman Diabetes Institute, Kuwait City, Dasman 15462, Kuwait.

Ashraf Al Madhoun (A)

Genetics and Bioinformatics Department, Dasman Diabetes Institute, Kuwait City, Dasman 15462, Kuwait.
Animal and Imaging Core Facilities, Dasman Diabetes Institute, Kuwait City, Dasman 15462, Kuwait.

Sardar Sindhu (S)

Immunology & Microbiology Department, Dasman Diabetes Institute, Kuwait City, Dasman 15462, Kuwait.
Animal and Imaging Core Facilities, Dasman Diabetes Institute, Kuwait City, Dasman 15462, Kuwait.

Texy Jacob (T)

Immunology & Microbiology Department, Dasman Diabetes Institute, Kuwait City, Dasman 15462, Kuwait.

Shihab Kochumon (S)

Immunology & Microbiology Department, Dasman Diabetes Institute, Kuwait City, Dasman 15462, Kuwait.

Lina M Obeid (LM)

Stony Brook Cancer Center, Stony Brook University, Stony Brook, NY 11794, USA.

Fahd Al-Mulla (F)

Genetics and Bioinformatics Department, Dasman Diabetes Institute, Kuwait City, Dasman 15462, Kuwait.

Yusuf A Hannun (YA)

Stony Brook Cancer Center, Stony Brook University, Stony Brook, NY 11794, USA.

Rasheed Ahmad (R)

Immunology & Microbiology Department, Dasman Diabetes Institute, Kuwait City, Dasman 15462, Kuwait.

Classifications MeSH