Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
07 07 2023
07 07 2023
Historique:
received:
10
02
2023
accepted:
16
06
2023
medline:
10
7
2023
pubmed:
8
7
2023
entrez:
7
7
2023
Statut:
epublish
Résumé
Cellular organization and functions encompass multiple scales in vivo. Emerging high-plex imaging technologies are limited in resolving subcellular biomolecular features. Expansion Microscopy (ExM) and related techniques physically expand samples for enhanced spatial resolution, but are challenging to be combined with high-plex imaging technologies to enable integrative multiscaled tissue biology insights. Here, we introduce Expand and comPRESS hydrOgels (ExPRESSO), an ExM framework that allows high-plex protein staining, physical expansion, and removal of water, while retaining the lateral tissue expansion. We demonstrate ExPRESSO imaging of archival clinical tissue samples on Multiplexed Ion Beam Imaging and Imaging Mass Cytometry platforms, with detection capabilities of > 40 markers. Application of ExPRESSO on archival human lymphoid and brain tissues resolved tissue architecture at the subcellular level, particularly that of the blood-brain barrier. ExPRESSO hence provides a platform for extending the analysis compatibility of hydrogel-expanded biospecimens to mass spectrometry, with minimal modifications to protocols and instrumentation.
Identifiants
pubmed: 37419873
doi: 10.1038/s41467-023-39616-w
pii: 10.1038/s41467-023-39616-w
pmc: PMC10329015
doi:
Substances chimiques
Proteins
0
Hydrogels
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4013Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM137936
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007290
Pays : United States
Organisme : NIAID NIH HHS
ID : DP2 AI171139
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG068279
Pays : United States
Organisme : NICHD NIH HHS
ID : P01 HD106414
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG056287
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG057915
Pays : United States
Organisme : NIA NIH HHS
ID : U19 AG065156
Pays : United States
Organisme : NCI NIH HHS
ID : F31 CA246880
Pays : United States
Organisme : Bill & Melinda Gates Foundation
ID : INV-002704
Pays : United States
Organisme : NIA NIH HHS
ID : K08 AG065502
Pays : United States
Informations de copyright
© 2023. The Author(s).
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