Hyperglycemia is associated with duodenal dysbiosis and altered duodenal microenvironment.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
07 07 2023
07 07 2023
Historique:
received:
27
07
2022
accepted:
26
06
2023
medline:
10
7
2023
pubmed:
8
7
2023
entrez:
7
7
2023
Statut:
epublish
Résumé
The gut microbiome influences the pathogenesis and course of metabolic disorders such as diabetes. While it is likely that duodenal mucosa associated microbiota contributes to the genesis and progression of increased blood sugar, including the pre-diabetic stage, it is much less studied than stool. We investigated paired stool and duodenal microbiota in subjects with hyperglycemia (HbA1c ≥ 5.7% and fasting plasma glucose > 100 mg/dl) compared to normoglycemic. We found patients with hyperglycemia (n = 33) had higher duodenal bacterial count (p = 0.008), increased pathobionts and reduction in beneficial flora compared to normoglycemic (n = 21). The microenvironment of duodenum was assessed by measuring oxygen saturation using T-Stat, serum inflammatory markers and zonulin for gut permeability. We observed that bacterial overload was correlated with increased serum zonulin (p = 0.061) and higher TNF-α (p = 0.054). Moreover, reduced oxygen saturation (p = 0.021) and a systemic proinflammatory state [increased total leukocyte count (p = 0.031) and reduced IL-10 (p = 0.015)] characterized the duodenum of hyperglycemic. Unlike stool flora, the variability in duodenal bacterial profile was associated with glycemic status and was predicted by bioinformatic analysis to adversely affect nutrient metabolism. Our findings offer new understanding of the compositional changes in the small intestine bacteria by identifying duodenal dysbiosis and altered local metabolism as potentially early events in hyperglycemia.
Identifiants
pubmed: 37419941
doi: 10.1038/s41598-023-37720-x
pii: 10.1038/s41598-023-37720-x
pmc: PMC10329043
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11038Informations de copyright
© 2023. The Author(s).
Références
Genomics. 2020 Jul;112(4):2784-2793
pubmed: 32209379
Nat Med. 2019 Jul;25(7):1096-1103
pubmed: 31263284
Gut. 2022 Feb;71(2):254-264
pubmed: 33597157
Clin Gastroenterol Hepatol. 2022 Feb;20(2):e196-e227
pubmed: 33161160
Microbiome. 2018 Aug 29;6(1):150
pubmed: 30157953
PLoS One. 2020 Jul 9;15(7):e0234906
pubmed: 32645011
Nutrients. 2020 May 19;12(5):
pubmed: 32438689
Sci Rep. 2019 Jul 4;9(1):9685
pubmed: 31273267
Am J Physiol Endocrinol Metab. 2006 Mar;290(3):E550-9
pubmed: 16219666
Gut. 2020 Feb;69(2):295-303
pubmed: 31331994
Eur J Nutr. 2018 Dec;57(8):2985-2997
pubmed: 30043185
Cancer Epidemiol Biomarkers Prev. 2019 Feb;28(2):370-383
pubmed: 30373903
J Clin Microbiol. 2007 Oct;45(10):3474-5
pubmed: 17687015
Front Microbiol. 2019 Oct 01;10:2259
pubmed: 31632373
Clin Immunol. 2018 Dec;197:139-153
pubmed: 30240602
J Clin Endocrinol Metab. 2017 May 1;102(5):1468-1477
pubmed: 28324102
Endocrinol Diabetes Metab. 2020 Nov 11;4(1):e00162
pubmed: 33532605
N Engl J Med. 2020 Jul 23;383(4):321-333
pubmed: 32706533
Cell Metab. 2015 Sep 1;22(3):367-80
pubmed: 26212718
Gastroenterology. 2012 Oct;143(4):913-6.e7
pubmed: 22728514
NPJ Biofilms Microbiomes. 2015 Dec 16;1:15026
pubmed: 28721237
FEMS Immunol Med Microbiol. 2003 Oct 24;39(1):81-6
pubmed: 14557000
Front Microbiol. 2018 Apr 20;9:765
pubmed: 29731746
Nat Methods. 2016 Jul;13(7):581-3
pubmed: 27214047
Antioxid Redox Signal. 2014 Feb 10;20(5):818-30
pubmed: 23582008
J Microbiol Biotechnol. 2015 Jul;25(7):1136-45
pubmed: 25737115
Nat Rev Microbiol. 2016 Jan;14(1):20-32
pubmed: 26499895
Diabetes Care. 2005 Aug;28(8):2036-7
pubmed: 16043753
J Epidemiol Glob Health. 2020 Mar;10(1):107-111
pubmed: 32175717
Indian J Microbiol. 2019 Mar;59(1):90-95
pubmed: 30728635
Cell Rep. 2021 Sep 28;36(13):109765
pubmed: 34592155
Sci Rep. 2020 Sep 11;10(1):14977
pubmed: 32917913
F1000Res. 2020 Jan 31;9:
pubmed: 32051759
BMC Res Notes. 2016 Jun 28;9:328
pubmed: 27352784
J Int Med Res. 2020 Jul;48(7):300060520937866
pubmed: 32691685
Nat Commun. 2019 Jun 6;10(1):2474
pubmed: 31171772
Trends Endocrinol Metab. 2020 Nov;31(11):818-834
pubmed: 32284282
J Transl Med. 2021 Apr 28;19(1):177
pubmed: 33910577
Nat Med. 2015 May;21(5):498-505
pubmed: 25849131
Dig Dis Sci. 2020 Sep;65(9):2595-2604
pubmed: 32140945
Gastroenterology. 2002 Nov;123(5):1607-15
pubmed: 12404235
Int J Mol Sci. 2017 Mar 08;18(3):
pubmed: 28282855
BMC Gastroenterol. 2013 Jul 11;13:113
pubmed: 23844808
Proc Natl Acad Sci U S A. 2018 Apr 17;115(16):4170-4175
pubmed: 29610310
J Neurogastroenterol Motil. 2018 Jul 30;24(3):345-354
pubmed: 29791992
Microorganisms. 2020 Mar 29;8(4):
pubmed: 32235377
Diabetes. 2009 Nov;58(11):2525-35
pubmed: 19690064
Antioxidants (Basel). 2021 Dec 15;10(12):
pubmed: 34943104
Diabetes. 2007 Jul;56(7):1761-72
pubmed: 17456850
Obes Rev. 2022 Oct;23(10):e13498
pubmed: 35856338
Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4607-14
pubmed: 20826446
BMC Endocr Disord. 2020 Jul 22;20(1):108
pubmed: 32698783
Clin Transl Gastroenterol. 2019 Aug;10(8):e00073
pubmed: 31464691
Cell Metab. 2015 Aug 4;22(2):228-38
pubmed: 26244932
Sci Rep. 2018 Oct 10;8(1):15072
pubmed: 30305667
Cell Metab. 2018 Jan 9;27(1):101-117.e5
pubmed: 29056513
World J Gastroenterol. 2006 Sep 21;12(35):5611-21
pubmed: 17007012
Anesthesiology. 2004 Jun;100(6):1469-75
pubmed: 15166566
Nature. 2019 May;569(7758):663-671
pubmed: 31142858
Science. 2018 Mar 23;359(6382):1376-1383
pubmed: 29519916
Nat Commun. 2016 Nov 23;7:13419
pubmed: 27876802
J Dent Res. 2019 Dec;98(13):1488-1496
pubmed: 31623509