Ceramide releases exosomes with a specific miRNA signature for cell differentiation.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
07 07 2023
07 07 2023
Historique:
received:
13
03
2023
accepted:
30
06
2023
medline:
10
7
2023
pubmed:
8
7
2023
entrez:
7
7
2023
Statut:
epublish
Résumé
Exosomes are well established effectors of cell-cell communication. Their role on maturation of embryonic cells located in hippocampus, seat of memory, is unknown. Here we show that ceramide facilitates release of exosomes from HN9.10e cells extending information for cell differentiation to neighboring cells. We found only 38 miRNAs differentially expressed in exosomes derived from ceramide-treated cells in comparison with control cells (including 10 up-regulated and 28 down-regulated). Some overexpressed miRNAs (mmu-let-7f-1-3p, mmu-let-7a-1-3p, mmu-let-7b-3p, mmu-let-7b-5p, mmu-miR-330-3p) regulate genes encoding for protein involved in biological, homeostatic, biosynthetic and small molecule metabolic processes, embryo development and cell differentiation, all phenomena relevant for HN9.10e cell differentiation. Notably, the overexpressed mmu-let-7b-5p miRNA appears to be important for our study based on its ability to regulate thirty-five gene targets involved in many processes including sphingolipid metabolism, sphingolipid-related stimulation of cellular functions and neuronal development. Furthermore, we showed that by incubating embryonic cells with exosomes released under ceramide treatment, some cells acquired an astrocytic phenotype and others a neuronal phenotype. We anticipate our study to be a start point for innovative therapeutic strategies to regulate the release of exosomes useful to stimulate delayed brain development in the newborn and to improve the cognitive decline in neurodegenerative disorders.
Identifiants
pubmed: 37419964
doi: 10.1038/s41598-023-38011-1
pii: 10.1038/s41598-023-38011-1
pmc: PMC10329022
doi:
Substances chimiques
Ceramides
0
MicroRNAs
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
10993Informations de copyright
© 2023. The Author(s).
Références
J Biol Chem. 2003 May 30;278(22):19777-83
pubmed: 12649271
Int J Mol Sci. 2022 Sep 23;23(19):
pubmed: 36232480
Acta Pharmacol Sin. 2018 Apr;39(4):561-568
pubmed: 28858294
Nature. 2003 Dec 18;426(6968):803-9
pubmed: 14685229
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Int J Mol Sci. 2020 Jul 18;21(14):
pubmed: 32708414
Biochim Biophys Acta. 2005 Jun 1;1711(1):12-9
pubmed: 15904658
Nucleic Acids Res. 2015 Jul 1;43(W1):W460-6
pubmed: 25977294
Int J Mol Sci. 2022 Aug 26;23(17):
pubmed: 36077094
Nat Rev Neurosci. 2016 Mar;17(3):160-72
pubmed: 26891626
Front Mol Neurosci. 2022 Nov 09;15:1004221
pubmed: 36438184
Aging (Albany NY). 2018 Dec 12;10(12):3794-3805
pubmed: 30540563
Bioinformatics. 2009 Apr 15;25(8):1091-3
pubmed: 19237447
Front Cell Neurosci. 2018 Feb 07;12:31
pubmed: 29467625
J Neurosci Res. 2002 May 1;68(3):323-30
pubmed: 12111862
Mol Neurobiol. 2019 Aug;56(8):5436-5455
pubmed: 30612333
Neurosci Lett. 2014 Sep 19;580:158-62
pubmed: 25123444
Science. 2008 Feb 29;319(5867):1244-7
pubmed: 18309083
Mediators Inflamm. 2017;2017:2470950
pubmed: 29343884
Genome Biol. 2014;15(12):550
pubmed: 25516281
Prog Lipid Res. 2017 Apr;66:30-41
pubmed: 28342835
Front Neurol. 2020 May 21;11:437
pubmed: 32528400
Cancers (Basel). 2020 Oct 28;12(11):
pubmed: 33126474
J Lipid Res. 2018 Aug;59(8):1325-1340
pubmed: 29853528
Int J Nanomedicine. 2021 Feb 17;16:1281-1312
pubmed: 33628021
Int J Mol Sci. 2019 Jul 25;20(15):
pubmed: 31349547
Cells. 2019 Apr 03;8(4):
pubmed: 30987213
Curr Opin Cell Biol. 2014 Aug;29:116-25
pubmed: 24959705
J Lipid Res. 2005 May;46(5):839-61
pubmed: 15722563
Int J Mol Sci. 2021 Jan 23;22(3):
pubmed: 33498778
J Biol Chem. 2011 Aug 12;286(32):27855-62
pubmed: 21693702
Int J Mol Sci. 2022 Sep 15;23(18):
pubmed: 36142663
Biochim Biophys Acta. 2013 Jul;1831(7):1302-9
pubmed: 24046871
Oncotarget. 2017 Dec 24;9(5):5578-5587
pubmed: 29464019
Rev Neurosci. 2020 Oct 25;31(7):743-756
pubmed: 32681787
Genome Res. 2003 Nov;13(11):2498-504
pubmed: 14597658
Methods Mol Biol. 2023;2595:13-47
pubmed: 36441452
Methods Mol Biol. 2018;1697:153-171
pubmed: 28540559
Bioinformatics. 2016 Oct 1;32(19):3047-8
pubmed: 27312411
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2022 Aug;34(8):842-847
pubmed: 36177928
Int J Mol Sci. 2020 Sep 03;21(17):
pubmed: 32899117
Cell Signal. 2021 Nov;87:110119
pubmed: 34418535
Nucleic Acids Res. 2016 Jan 4;44(D1):D457-62
pubmed: 26476454
Elife. 2022 Nov 25;11:
pubmed: 36426850
Mol Biol Cell. 2011 Sep;22(17):3022-31
pubmed: 21737687
Biochemistry. 1999 Nov 2;38(44):14676-82
pubmed: 10545193
Mediators Inflamm. 2018 Feb 6;2018:5378284
pubmed: 29540995
Methods Mol Biol. 2012;874:177-92
pubmed: 22528448
J Cell Physiol. 2019 May;234(5):5451-5465
pubmed: 30471116
J Neurosci Res. 1995 Apr 1;40(5):622-31
pubmed: 7541474
Mol Ther. 2022 Mar 2;30(3):1300-1314
pubmed: 34768001
J Control Release. 2017 Sep 28;262:247-258
pubmed: 28687495
Int J Mol Sci. 2021 Aug 27;22(17):
pubmed: 34502192
Mol Neurobiol. 2021 Jan;58(1):329-347
pubmed: 32944919
Nat Commun. 2022 Feb 17;13(1):936
pubmed: 35177612
Nucleic Acids Res. 2000 Jan 1;28(1):27-30
pubmed: 10592173
Cells. 2022 May 25;11(11):
pubmed: 35681438
PLoS Med. 2020 Dec 9;17(12):e1003451
pubmed: 33296380
Neuropsychiatr Dis Treat. 2015 Jul 14;11:1695-701
pubmed: 26203251
Front Biosci (Landmark Ed). 2022 Aug 17;27(8):247
pubmed: 36042186