Discovery and optimized extraction of the anti-osteoclastic agent epicatechin-7-O-β-D-apiofuranoside from Ulmus macrocarpa Hance bark.

Mice Animals Osteoclasts Catechin / pharmacology Ulmus Plant Bark Plant Extracts / pharmacology Ethanol / pharmacology Cell Differentiation RANK Ligand / pharmacology

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
09 07 2023
Historique:
received: 20 10 2022
accepted: 05 07 2023
medline: 11 7 2023
pubmed: 10 7 2023
entrez: 9 7 2023
Statut: epublish

Résumé

Ulmus macrocarpa Hance bark (UmHb) has been used as a traditional herbal medicine in East Asia for bone concern diseases for a long time. To find a suitable solvent, we, in this study, compared the efficacy of UmHb water extract and ethanol extract which can inhibit osteoclast differentiation. Compared with two ethanol extracts (70% and 100% respectively), hydrothermal extracts of UmHb more effectively inhibited receptor activators of nuclear factor κB ligand-induced osteoclast differentiation in murine bone marrow-derived macrophages. We identified for the first time that (2R,3R)-epicatechin-7-O-β-D-apiofuranoside (E7A) is a specific active compound in UmHb hydrothermal extracts through using LC/MS, HPLC, and NMR techniques. In addition, we confirmed through TRAP assay, pit assay, and PCR assay that E7A is a key compound in inhibiting osteoclast differentiation. The optimized condition to obtain E7A-rich UmHb extract was 100 mL/g, 90 °C, pH 5, and 97 min. At this condition, the content of E7A was 26.05 ± 0.96 mg/g extract. Based on TRAP assay, pit assay, PCR, and western blot, the optimized extract of E7A-rich UmHb demonstrated a greater inhibition of osteoclast differentiation compared to unoptimized. These results suggest that E7A would be a good candidate for the prevention and treatment of osteoporosis-related diseases.

Identifiants

DOI: 10.1038/s41598-023-38208-4 PMID: 37423923 PMC: PMC10330169
pubmed: 37423923
doi: 10.1038/s41598-023-38208-4
pii: 10.1038/s41598-023-38208-4
pmc: PMC10330169
doi:

Substances chimiques

Catechin 8R1V1STN48
Plant Extracts 0
Ethanol 3K9958V90M
RANK Ligand 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

11102

Informations de copyright

© 2023. The Author(s).

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Auteurs

Chanhyeok Jeong (C)

Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Korea.

Yeon-Jin Cho (YJ)

Bio-MAX Institute, Seoul National University, Seoul, 08826, Korea.

Yongjin Lee (Y)

Department of Pharmacy, Sunchon National University, 315 Maegok-dong, Suncheon, Jeollanam-do, 57922, Korea.

Weihong Wang (W)

Laboratory of Marine Drugs, School of Earth and Environmental Sciences, Seoul National University, NS-80, Seoul, 08826, Korea.
Research Institute of Oceanography, Seoul National University, NS-80, Seoul, 08826, Korea.

Kyu-Hyung Park (KH)

Laboratory of Marine Drugs, School of Earth and Environmental Sciences, Seoul National University, NS-80, Seoul, 08826, Korea.

Eun Roh (E)

Laboratory of Marine Drugs, School of Earth and Environmental Sciences, Seoul National University, NS-80, Seoul, 08826, Korea.

Chang Hyung Lee (CH)

Bio-MAX Institute, Seoul National University, Seoul, 08826, Korea.

Young-Jin Son (YJ)

Department of Pharmacy, Sunchon National University, 315 Maegok-dong, Suncheon, Jeollanam-do, 57922, Korea.

Jung Han Yoon Park (JHY)

Bio-MAX Institute, Seoul National University, Seoul, 08826, Korea.

Heonjoong Kang (H)

Bio-MAX Institute, Seoul National University, Seoul, 08826, Korea.
Laboratory of Marine Drugs, School of Earth and Environmental Sciences, Seoul National University, NS-80, Seoul, 08826, Korea.
Research Institute of Oceanography, Seoul National University, NS-80, Seoul, 08826, Korea.
Interdisciplinary Graduate Program in Genetic Engineering, Seoul National University, NS-80, Seoul, 08826, Korea.

Ki Won Lee (KW)

Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Korea. kiwon@snu.ac.kr.
Bio-MAX Institute, Seoul National University, Seoul, 08826, Korea. kiwon@snu.ac.kr.
Advanced Institutes of Convergence Technology, Seoul National University, Suwon, 16229, Korea. kiwon@snu.ac.kr.
Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang, 25354, Korea. kiwon@snu.ac.kr.
Department of Agricultural Biotechnology and Center for Food and Bio convergence, Seoul National University, Seoul, 08826, Korea. kiwon@snu.ac.kr.

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Classifications MeSH

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