Monitoring of Rho GTPase Activity in Podocytes.

Podocytes / metabolism rho GTP-Binding Proteins / metabolism Endothelial Cells / metabolism Kidney Glomerulus / metabolism Actin Cytoskeleton / metabolism rac1 GTP-Binding Protein / metabolism
Cdc42 Podocyte Pull-down Rac1 Rho GTPase RhoA

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2023
Historique:
medline: 11 7 2023
pubmed: 10 7 2023
entrez: 9 7 2023
Statut: ppublish

Résumé

The glomerulus is the filtration unit of the kidney, where the first step of urine formation occurs. Podocytes are characterized by their actin-based projections called foot processes. Podocyte foot processes play a critical role in the permselective filtration barrier, along with fenestrated endothelial cells and the glomerular basement membrane. The Rho family of small GTPases (Rho GTPases) is the master regulators of the actin cytoskeleton and functions as molecular switches. Recent studies have shown that disruption of Rho GTPase activity and subsequent changes in foot process structure cause proteinuria. Here, we describe an effector pull-down assay using GST-fusion proteins to monitor the activity of RhoA, Rac1, and Cdc42, which are prototypical Rho GTPases in podocytes.

Identifiants

DOI: 10.1007/978-1-0716-3179-9_22 PMID: 37423999
pubmed: 37423999
doi: 10.1007/978-1-0716-3179-9_22
doi:

Substances chimiques

rho GTP-Binding Proteins EC 3.6.5.2
rac1 GTP-Binding Protein EC 3.6.5.2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

343-349

Subventions

Organisme : CIHR
ID : PJT-159678
Pays : Canada

Informations de copyright

© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

Heasman SJ, Ridley AJ (2008) Mammalian Rho GTPases: new insights into their functions from in vivo studies. Nat Rev Mol Cell Biol 9:690–701. https://doi.org/10.1038/nrm2476
doi: 10.1038/nrm2476 pubmed: 18719708
Mouawad F, Tsui H, Takano T (2013) Role of Rho-GTPases and their regulatory proteins in glomerular podocyte function. Can J Physiol Pharmacol 91:773–782. https://doi.org/10.1139/cjpp-2013-0135
doi: 10.1139/cjpp-2013-0135 pubmed: 24144047
Matsuda J, Asano-Matsuda K, Kitzler T et al (2021) Rho GTPase regulatory proteins in podocytes. Kidney Int 99:336–345. https://doi.org/10.1016/j.kint.2020.08.035
doi: 10.1016/j.kint.2020.08.035 pubmed: 33122025
Asano-Matsuda K, Ibrahim S, Takano T et al (2021) Role of Rho GTPase interacting proteins in subcellular compartments of podocytes. Int J Mol Sci 22:3656. https://doi.org/10.3390/ijms22073656
doi: 10.3390/ijms22073656 pubmed: 33915776 pmcid: 8037304
Steichen C, Herve JC, Hauet T et al (2022) Rho GTPases in kidney physiology and diseases. Small GTPases 13(1):141–161. https://doi.org/10.1080/21541248.2021.1932402
doi: 10.1080/21541248.2021.1932402 pubmed: 34138686
Scott RP, Hawley SP, Ruston J et al (2012) Podocyte-specific loss of Cdc42 leads to congenital nephropathy. J Am Soc Nephrol 23:1149–1154. https://doi.org/10.1681/ASN.2011121206
doi: 10.1681/ASN.2011121206 pubmed: 22518006 pmcid: 3380653
Blattner SM, Hodgin JB, Nishio M et al (2013) Divergent functions of the Rho GTPases Rac1 and Cdc42 in podocyte injury. Kidney Int 84:920–930. https://doi.org/10.1038/ki.2013.175
doi: 10.1038/ki.2013.175 pubmed: 23677246 pmcid: 3815690
Wang L, Ellis MJ, Gomez JA et al (2012) Mechanisms of the proteinuria induced by Rho GTPases. Kidney Int 81:1075–1085. https://doi.org/10.1038/ki.2011.472
doi: 10.1038/ki.2011.472 pubmed: 22278020 pmcid: 3352980
Zhu L, Jiang R, Aoudjit L et al (2011) Activation of RhoA in podocytes induces focal segmental glomerulosclerosis. J Am Soc Nephrol 22:1621–1630. https://doi.org/10.1681/ASN.2010111146
doi: 10.1681/ASN.2010111146 pubmed: 21804090 pmcid: 3171934
Yu H, Suleiman H, Kim A et al (2013) Rac1 activation in podocytes induces rapid foot process effacement and proteinuria. Mol Cell Biol 33:4755–4764
doi: 10.1128/MCB.00730-13 pubmed: 24061480 pmcid: 3838009
Robins R, Baldwin C, Aoudjit L et al (2017) Rac1 activation in podocytes induces the spectrum of nephrotic syndrome. Kidney Int 92:349–364. https://doi.org/10.1016/j.kint.2017.03.010
doi: 10.1016/j.kint.2017.03.010 pubmed: 28483380
Reid T, Furuyashiki T, Ishizaki T et al (1996) Rhotekin, a new putative target for Rho bearing homology to a serine/threonine kinase, PKN, and rhophilin in the rho-binding domain. J Biol Chem 271:13556–13560. https://doi.org/10.1074/jbc.271.23.13556
doi: 10.1074/jbc.271.23.13556 pubmed: 8662891
Burbelo PD, Drechsel D, Hall A (1995) A conserved binding motif defines numerous candidate target proteins for both Cdc42 and Rac GTPases. J Biol Chem 270:29071–29074. https://doi.org/10.1074/jbc.270.49.29071
doi: 10.1074/jbc.270.49.29071 pubmed: 7493928

Auteurs

Jun Matsuda (J)

Department of Nephrology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan. matsuda@kid.med.osaka-u.ac.jp.

Tomoko Takano (T)

Division of Nephrology, McGill University Health Centre, Montreal, QC, Canada.

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules épithéliales › Podocytes › Monitoring of Rho GTPase Activity in Podocytes.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines G › Protéines G monomériques › Protéines G rho › Monitoring of Rho GTPase Activity in Podocytes.
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules endothéliales › Monitoring of Rho GTPase Activity in Podocytes.
questionsmedicales.fr › Appareil urogénital › Voies urinaires › Rein › Néphrons › Glomérule rénal › Monitoring of Rho GTPase Activity in Podocytes.
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Cytosquelette › Cytosquelette d'actine › Monitoring of Rho GTPase Activity in Podocytes.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines G › Protéines G monomériques › Protéines G rho › Protéines G rac › Protéine G rac1 › Monitoring of Rho GTPase Activity in Podocytes.