Sensing and avoiding sick conspecifics requires Gαi2
Avoidance behavior
Ca2+ imaging
Dendritic knob
Gαi2
Inflammation
LPS
Lateral habenula
Olfactory
V1R
Vomeronasal organ
Journal
BMC biology
ISSN: 1741-7007
Titre abrégé: BMC Biol
Pays: England
ID NLM: 101190720
Informations de publication
Date de publication:
10 07 2023
10 07 2023
Historique:
received:
24
01
2023
accepted:
23
06
2023
medline:
11
7
2023
pubmed:
10
7
2023
entrez:
9
7
2023
Statut:
epublish
Résumé
Rodents utilize chemical cues to recognize and avoid other conspecifics infected with pathogens. Infection with pathogens and acute inflammation alter the repertoire and signature of olfactory stimuli emitted by a sick individual. These cues are recognized by healthy conspecifics via the vomeronasal or accessory olfactory system, triggering an innate form of avoidance behavior. However, the molecular identity of the sensory neurons and the higher neural circuits involved in the detection of sick conspecifics remain poorly understood. We employed mice that are in an acute state of inflammation induced by systemic administration of lipopolysaccharide (LPS). Through conditional knockout of the G-protein Gαi2 and deletion of other key sensory transduction molecules (Trpc2 and a cluster of 16 vomeronasal type 1 receptors), in combination with behavioral testing, subcellular Ca Our physiological and behavioral analyses indicate that the sensing and avoidance of LPS-treated sick conspecifics depend on the Gαi2 vomeronasal subsystem. Our observations point to a central role of brain circuits downstream of the olfactory periphery and in the lateral habenula in the detection and avoidance of sick conspecifics, providing new insights into the neural substrates and circuit logic of the sensing of inflammation in mice.
Sections du résumé
BACKGROUND
Rodents utilize chemical cues to recognize and avoid other conspecifics infected with pathogens. Infection with pathogens and acute inflammation alter the repertoire and signature of olfactory stimuli emitted by a sick individual. These cues are recognized by healthy conspecifics via the vomeronasal or accessory olfactory system, triggering an innate form of avoidance behavior. However, the molecular identity of the sensory neurons and the higher neural circuits involved in the detection of sick conspecifics remain poorly understood.
RESULTS
We employed mice that are in an acute state of inflammation induced by systemic administration of lipopolysaccharide (LPS). Through conditional knockout of the G-protein Gαi2 and deletion of other key sensory transduction molecules (Trpc2 and a cluster of 16 vomeronasal type 1 receptors), in combination with behavioral testing, subcellular Ca
CONCLUSIONS
Our physiological and behavioral analyses indicate that the sensing and avoidance of LPS-treated sick conspecifics depend on the Gαi2 vomeronasal subsystem. Our observations point to a central role of brain circuits downstream of the olfactory periphery and in the lateral habenula in the detection and avoidance of sick conspecifics, providing new insights into the neural substrates and circuit logic of the sensing of inflammation in mice.
Identifiants
pubmed: 37424020
doi: 10.1186/s12915-023-01653-8
pii: 10.1186/s12915-023-01653-8
pmc: PMC10332101
doi:
Substances chimiques
Lipopolysaccharides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
152Informations de copyright
© 2023. The Author(s).
Références
Proc Natl Acad Sci U S A. 2009 Jun 16;106(24):9842-7
pubmed: 19497865
Nature. 2011 Sep 21;478(7368):241-5
pubmed: 21937988
Nat Neurosci. 2015 Oct;18(10):1446-54
pubmed: 26322927
eNeuro. 2021 Jun 22;8(3):
pubmed: 33962969
Neuron. 2016 Dec 21;92(6):1196-1203
pubmed: 27916458
Pharmacol Biochem Behav. 2002 Mar;71(3):481-91
pubmed: 11830182
Nat Commun. 2013;4:1616
pubmed: 23511480
Cell. 2015 Oct 8;163(2):313-23
pubmed: 26435105
J Biol Chem. 2015 Mar 20;290(12):7369-87
pubmed: 25605714
Horm Behav. 2004 Sep;46(3):272-83
pubmed: 15325228
Brain Res. 1996 May 6;719(1-2):117-28
pubmed: 8782871
Nature. 2006 Nov 16;444(7117):308-15
pubmed: 17108955
Curr Biol. 2015 May 18;25(10):1340-6
pubmed: 25936549
Sci Adv. 2020 May 29;6(22):eaaz6868
pubmed: 32523992
Cell. 2010 May 14;141(4):692-703
pubmed: 20478258
Philos Trans R Soc Lond B Biol Sci. 2018 Jul 19;373(1751):
pubmed: 29866919
J Neurosci. 2008 Jun 18;28(25):6407-18
pubmed: 18562612
Elife. 2021 Apr 26;10:
pubmed: 33900197
Curr Biol. 2015 Jan 19;25(2):251-255
pubmed: 25578906
Front Cell Neurosci. 2021 Feb 17;15:638800
pubmed: 33679330
Neuron. 2021 Aug 4;109(15):2469-2484.e7
pubmed: 34186026
Int J Mass Spectrom. 2018 Nov;434:185-192
pubmed: 30872949
Proc Natl Acad Sci U S A. 1999 May 11;96(10):5791-6
pubmed: 10318963
Chem Senses. 2018 Nov 1;43(9):667-695
pubmed: 30256909
Transl Psychiatry. 2022 Jan 10;12(1):3
pubmed: 35013094
Neurosci Biobehav Rev. 2020 Dec;119:281-293
pubmed: 33031813
Nat Commun. 2019 Oct 25;10(1):4889
pubmed: 31653840
Nat Neurosci. 2013 Dec;16(12):1731-3
pubmed: 24212674
Nature. 2002 Sep 5;419(6902):70-4
pubmed: 12214233
BMC Evol Biol. 2020 Aug 8;20(1):99
pubmed: 32770934
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):6376-81
pubmed: 11972034
Cell Rep. 2014 Jul 24;8(2):583-95
pubmed: 25001287
Sci Rep. 2018 May 31;8(1):8490
pubmed: 29855521
Sci Rep. 2017 Dec 4;7(1):16878
pubmed: 29203879
Nature. 2010 Jul 1;466(7302):118-22
pubmed: 20596023
Sci Rep. 2018 Sep 24;8(1):14255
pubmed: 30250285
Nature. 2009 May 28;459(7246):574-7
pubmed: 19387439
Neuron. 2003 Oct 30;40(3):551-61
pubmed: 14642279
Proc Natl Acad Sci U S A. 2011 Aug 2;108(31):12898-903
pubmed: 21768373
Vet Res. 2016 Jan 07;47:11
pubmed: 26738723
Cell Tissue Res. 2021 Jan;383(1):387-393
pubmed: 33452930
Nat Rev Neurosci. 2020 May;21(5):277-295
pubmed: 32269316
Cell. 2021 Feb 4;184(3):615-627.e17
pubmed: 33453153
Nat Rev Neurosci. 2008 Jan;9(1):46-56
pubmed: 18073775
Elife. 2014 Jul 29;3:e03025
pubmed: 25073926
Cell Tissue Res. 2021 Jan;383(1):367-386
pubmed: 33433690
Annu Rev Physiol. 2014;76:151-75
pubmed: 23988175
Nature. 2007 Dec 6;450(7171):899-902
pubmed: 18064011
Nature. 2021 May;593(7857):114-118
pubmed: 33790466
Sci Rep. 2020 Jan 21;10(1):894
pubmed: 31965032
Proc Natl Acad Sci U S A. 2019 Mar 12;116(11):5135-5143
pubmed: 30804203
Science. 2002 Feb 22;295(5559):1493-500
pubmed: 11823606
Nat Commun. 2016 Jun 21;7:11936
pubmed: 27324439
Neurosci Biobehav Rev. 2011 Oct;35(9):1916-28
pubmed: 21414355
Cell. 2018 Dec 13;175(7):1827-1841.e17
pubmed: 30550786
Neurosci Biobehav Rev. 2017 May;76(Pt A):39-47
pubmed: 28434586
Cells. 2019 Dec 12;8(12):
pubmed: 31842409