A computational suite for the structural and functional characterization of amyloid aggregates.
Image processing
analysis automation
fluorescence microscopy
neurodegenerative disease
protein aggregate characterisation
super-resolution imaging
Journal
Cell reports methods
ISSN: 2667-2375
Titre abrégé: Cell Rep Methods
Pays: United States
ID NLM: 9918227360606676
Informations de publication
Date de publication:
26 06 2023
26 06 2023
Historique:
received:
24
10
2022
revised:
11
04
2023
accepted:
17
05
2023
medline:
11
7
2023
pubmed:
10
7
2023
entrez:
10
7
2023
Statut:
epublish
Résumé
We developed the aggregate characterization toolkit (ACT), a fully automated computational suite based on existing and widely used core algorithms to measure the number, size, and permeabilizing activity of recombinant and human-derived aggregates imaged with diffraction-limited and super-resolution microscopy methods at high throughput. We have validated ACT on simulated ground-truth images of aggregates mimicking those from diffraction-limited and super-resolution microscopies and showcased its use in characterizing protein aggregates from Alzheimer's disease. ACT is developed for high-throughput batch processing of images collected from multiple samples and is available as an open-source code. Given its accuracy, speed, and accessibility, ACT is expected to be a fundamental tool in studying human and non-human amyloid intermediates, developing early disease stage diagnostics, and screening for antibodies that bind toxic and heterogeneous human amyloid aggregates.
Identifiants
pubmed: 37426747
doi: 10.1016/j.crmeth.2023.100499
pii: S2667-2375(23)00128-5
pmc: PMC10326375
doi:
Substances chimiques
Protein Aggregates
0
Amyloid
0
Amyloidogenic Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
100499Subventions
Organisme : Medical Research Council
Pays : United Kingdom
Informations de copyright
© 2023 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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