TUNEL Staining in Sections of Paraffin-Enabled Planarians.
Apoptosis
Cell death
DNA fragmentation
Paraffin sections
Planarian
TUNEL
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
medline:
12
7
2023
pubmed:
10
7
2023
entrez:
10
7
2023
Statut:
ppublish
Résumé
Planarians are a model animal for the study of regeneration and homeostasis. Understanding how planarians control their cellular balance is key to the knowledge of their plasticity. Both apoptotic and mitotic rates can be quantified in "whole mount" planarians. Apoptosis is usually analyzed through terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), a technique that detects cell death by identifying DNA breaks. In this chapter we detail a protocol to analyze apoptotic cells in paraffin sections of planarians, which enables a more accurate cellular visualization and quantification than in "whole mount."
Identifiants
pubmed: 37428382
doi: 10.1007/978-1-0716-3275-8_15
doi:
Substances chimiques
Paraffin
8002-74-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
245-251Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
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pubmed: 29916169
pmcid: 6042512
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