An ultrasensitive aptasensor for exosomes detection based on biotin-streptavidin and MXenes.
Colorimetric aptasensor
Dual-effect amplification method
Exosomes detection
Lung cancer
Journal
Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535
Informations de publication
Date de publication:
01 09 2023
01 09 2023
Historique:
received:
11
04
2023
revised:
10
06
2023
accepted:
03
07
2023
medline:
27
7
2023
pubmed:
12
7
2023
entrez:
11
7
2023
Statut:
ppublish
Résumé
Exosomes derived from lung cancer typically contain the genetic information of the donor cells. Therefore, exosomes contribute to early diagnosis, treatment effectiveness evaluation, and prognosis assessment of cancer. Based on the biotin-streptavidin system and MXenes nanomaterial, a dual-effect amplification method had been developed to construct an ultrasensitive colorimetric aptasensor for detecting exosomes. MXenes can enhance the loading of aptamer and biotin as the high specific surface area. Biotin-streptavidin system can increase the amount of horseradish peroxidase-linked (HRP-linked) streptavidin, considerably boosting the color signal of the aptasensor. The proposed colorimetric aptasensor exhibited excellent sensitivity, with a detection limit of 42 particles μL
Identifiants
pubmed: 37433362
pii: S0003-2697(23)00198-7
doi: 10.1016/j.ab.2023.115233
pii:
doi:
Substances chimiques
Streptavidin
9013-20-1
Biotin
6SO6U10H04
Aptamers, Nucleotide
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
115233Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.