Particulate matter 10 exposure affects intestinal functionality in both inflamed 2D intestinal epithelial cell and 3D intestinal organoid models.
absorptive function
calcium signaling
human intestinal epithelium
human intestinal organoid
inflammation
particulate matter 10
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2023
2023
Historique:
received:
17
02
2023
accepted:
30
05
2023
medline:
13
7
2023
pubmed:
12
7
2023
entrez:
12
7
2023
Statut:
epublish
Résumé
A growing body of evidence suggests that particulate matter (PM10) enters the gastrointestinal (GI) tract directly, causing the GI epithelial cells to function less efficiently, leading to inflammation and an imbalance in the gut microbiome. PM10 may, however, act as an exacerbation factor in patients with inflamed intestinal epithelium, which is associated with inflammatory bowel disease. The purpose of this study was to dissect the pathology mechanism of PM10 exposure in inflamed intestines. In this study, we established chronically inflamed intestinal epithelium models utilizing two-dimensional (2D) human intestinal epithelial cells (hIECs) and 3D human intestinal organoids (hIOs), which mimic Inflamed 2D hIECs and 3D hIOs exhibited pathological features, such as inflammation, decreased intestinal markers, and defective epithelial barrier function. In addition, we found that PM10 exposure induced a more severe disturbance of peptide uptake in inflamed 2D hIECs and 3D hIOs than in control cells. This was due to the fact that it interferes with calcium signaling, protein digestion, and absorption pathways. The findings demonstrate that PM10-induced epithelial alterations contribute to the exacerbation of inflammatory disorders caused by the intestine. According to our findings, 2D hIEC and 3D hIO models could be powerful
Sections du résumé
Background
A growing body of evidence suggests that particulate matter (PM10) enters the gastrointestinal (GI) tract directly, causing the GI epithelial cells to function less efficiently, leading to inflammation and an imbalance in the gut microbiome. PM10 may, however, act as an exacerbation factor in patients with inflamed intestinal epithelium, which is associated with inflammatory bowel disease.
Objective
The purpose of this study was to dissect the pathology mechanism of PM10 exposure in inflamed intestines.
Methods
In this study, we established chronically inflamed intestinal epithelium models utilizing two-dimensional (2D) human intestinal epithelial cells (hIECs) and 3D human intestinal organoids (hIOs), which mimic
Results
Inflamed 2D hIECs and 3D hIOs exhibited pathological features, such as inflammation, decreased intestinal markers, and defective epithelial barrier function. In addition, we found that PM10 exposure induced a more severe disturbance of peptide uptake in inflamed 2D hIECs and 3D hIOs than in control cells. This was due to the fact that it interferes with calcium signaling, protein digestion, and absorption pathways. The findings demonstrate that PM10-induced epithelial alterations contribute to the exacerbation of inflammatory disorders caused by the intestine.
Conclusions
According to our findings, 2D hIEC and 3D hIO models could be powerful
Identifiants
pubmed: 37435069
doi: 10.3389/fimmu.2023.1168064
pmc: PMC10331606
doi:
Substances chimiques
Particulate Matter
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1168064Informations de copyright
Copyright © 2023 Son, Son, Yu, Baek, Park, Lee, Lee, Kim and Son.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
J Pharm Sci. 2004 Jul;93(7):1743-54
pubmed: 15176063
Food Funct. 2017 Jan 25;8(1):406-414
pubmed: 28091645
J Biol Chem. 2011 Aug 26;286(34):30232-43
pubmed: 21737448
Inflamm Bowel Dis. 2011 May;17(5):1138-45
pubmed: 20806342
Environ Health Perspect. 2014 Jan;122(1):A6-7
pubmed: 24380958
Food Addit Contam. 2005 Jul;22(7):624-41
pubmed: 16019838
J Biol Chem. 1996 Jun 7;271(23):13636-41
pubmed: 8662885
Nature. 2011 Feb 3;470(7332):105-9
pubmed: 21151107
Nat Commun. 2018 Aug 2;9(1):3039
pubmed: 30072687
Cell Mol Gastroenterol Hepatol. 2015 Mar 1;1(2):154-170
pubmed: 26000334
Environ Pollut. 2018 Sep;240:817-830
pubmed: 29783199
Proc Natl Acad Sci U S A. 1983 Sep;80(17):5397-401
pubmed: 6193516
Nat Med. 2011 Nov 20;17(12):1594-601
pubmed: 22101767
Gut. 2013 Jul;62(7):1062-71
pubmed: 22689519
Metabolites. 2022 May 07;12(5):
pubmed: 35629924
Sci Adv. 2021 Jun 2;7(23):
pubmed: 34078609
Front Physiol. 2016 Jun 21;7:245
pubmed: 27458380
World J Gastroenterol. 2014 Feb 7;20(5):1165-79
pubmed: 24574793
Oncotarget. 2017 Oct 12;8(60):102681-102689
pubmed: 29254280
Cell Calcium. 2014 Jun;55(6):376-84
pubmed: 24598807
Drug Metab Dispos. 2016 Dec;44(12):1890-1898
pubmed: 27638507
Molecules. 2020 Feb 26;25(5):
pubmed: 32111060
Part Fibre Toxicol. 2020 Jun 1;17(1):18
pubmed: 32487172
Eur Respir J. 2000 Sep;16(3):391-6
pubmed: 11028649
Am J Gastroenterol. 2010 Nov;105(11):2412-9
pubmed: 20588264
J Cell Biol. 2022 Apr 4;221(4):
pubmed: 35254388
Gastroenterology. 2013 Aug;145(2):407-15
pubmed: 23619146
Int J Mol Sci. 2016 Oct 10;17(10):
pubmed: 27735862
Int J Environ Res Public Health. 2020 May 06;17(9):
pubmed: 32384765
Part Fibre Toxicol. 2017 Nov 22;14(1):46
pubmed: 29166940
Front Neurol. 2021 May 21;12:651084
pubmed: 34093398
Environ Health Perspect. 2009 Nov;117(11):1780-5
pubmed: 20049132
Am J Physiol Gastrointest Liver Physiol. 2015 May 15;308(10):G874-83
pubmed: 25792563
Cell. 1993 Oct 22;75(2):263-74
pubmed: 8402911
Cell Death Dis. 2021 Sep 23;12(10):864
pubmed: 34556638
Physiol Rep. 2020 Jan;8(1):e14337
pubmed: 31960592
World J Gastroenterol. 2017 Apr 28;23(16):2841-2853
pubmed: 28522903
PLoS One. 2013 Apr 24;8(4):e62220
pubmed: 23638009
CMAJ. 2009 Oct 27;181(9):591-7
pubmed: 19805497
Sci Total Environ. 2012 Mar 1;419:25-36
pubmed: 22285091
Mol Nutr Food Res. 2019 Mar;63(5):e1801094
pubmed: 30521147
Stem Cells. 2013 Jul;31(7):1383-95
pubmed: 23553791
Inflamm Bowel Dis. 2014 Jul;20(7):1129-38
pubmed: 24859295
J Med Toxicol. 2012 Jun;8(2):166-75
pubmed: 22194192
J Thorac Dis. 2016 Jan;8(1):E8-E19
pubmed: 26904258
J Pharm Pharmacol. 2002 Sep;54(9):1293-6
pubmed: 12356285
Immunity. 2010 Mar 26;32(3):392-402
pubmed: 20303298
Part Fibre Toxicol. 2011 Jun 09;8:19
pubmed: 21658250
Gut Microbes. 2014 Mar-Apr;5(2):215-9
pubmed: 24637593
Lancet. 2017 May 13;389(10082):1907-1918
pubmed: 28408086
Drug Des Devel Ther. 2017 Dec 08;11:3511-3517
pubmed: 29263649
Gastroenterology. 2011 May;140(6):1756-1767
pubmed: 21530742
World J Gastroenterol. 2017 Sep 7;23(33):6016-6029
pubmed: 28970718
J Appl Physiol (1985). 2004 Dec;97(6):2200-6
pubmed: 15347631