PCR-Free Innovative Strategies for SARS-CoV-2 Detection.


Journal

Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613

Informations de publication

Date de publication:
10 2023
Historique:
revised: 24 04 2023
received: 16 02 2023
medline: 9 10 2023
pubmed: 12 7 2023
entrez: 12 7 2023
Statut: ppublish

Résumé

The pandemic outbreak caused by SARS-CoV-2 coronavirus brought a crucial issue in public health causing up to now more than 600 million infected people and 6.5 million deaths. Conventional diagnostic methods are based on quantitative reverse transcription polymerase chain reaction (RT-qPCR assay) and immuno-detection (ELISA assay). However, despite these techniques have the advantages of being standardized and consolidated, they keep some main limitations in terms of accuracy (immunoassays), time/cost consumption of analysis, the need for qualified personnel, and lab constrain (molecular assays). There is crucial the need to develop new diagnostic approaches for accurate, fast and portable viral detection and quantification. Among these, PCR-free biosensors represent the most appealing solution since they can allow molecular detection without the complexity of the PCR. This will enable the possibility to be integrated in portable and low-cost systems for massive and decentralized screening of SARS-CoV-2 in a point-of-care (PoC) format, pointing to achieve a performant identification and control of infection. In this review, the most recent approaches for the SARS-CoV-2 PCR-free detection are reported, describing both the instrumental and methodological features, and highlighting their suitability for a PoC application.

Identifiants

pubmed: 37435997
doi: 10.1002/adhm.202300512
doi:

Types de publication

Journal Article Review Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2300512

Informations de copyright

© 2023 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.

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Auteurs

Paolo Calorenni (P)

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno D'Alcontres 37, Messina, 98158, Italy.

Antonio A Leonardi (AA)

Department of Physics and Astronomy, University of Catania, Via Santa Sofia 64, Catania, 95123, Italy.

Emanuele L Sciuto (EL)

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno D'Alcontres 37, Messina, 98158, Italy.

Maria G Rizzo (MG)

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno D'Alcontres 37, Messina, 98158, Italy.

Maria J Lo Faro (MJL)

Department of Physics and Astronomy, University of Catania, Via Santa Sofia 64, Catania, 95123, Italy.

Barbara Fazio (B)

URT Lab Sens Beyond Nano, CNR-DSFTM, Viale F. Stagno D'Alcontres 37, Messina, 98158, Italy.

Alessia Irrera (A)

URT Lab Sens Beyond Nano, CNR-DSFTM, Viale F. Stagno D'Alcontres 37, Messina, 98158, Italy.

Sabrina Conoci (S)

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno D'Alcontres 37, Messina, 98158, Italy.
URT Lab Sens Beyond Nano, CNR-DSFTM, Viale F. Stagno D'Alcontres 37, Messina, 98158, Italy.
Department of Chemistry ''Giacomo Ciamician'', University of Bologna, Via Selmi 2, Bologna, 40126, Italy.
CNR-IMM, Institute for Microelectronics and Microsystems, Ottava Strada n.5, Catania, I-95121, Italy.

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