Regeneration of tracheal neotissue in partially decellularized scaffolds.
Journal
NPJ Regenerative medicine
ISSN: 2057-3995
Titre abrégé: NPJ Regen Med
Pays: United States
ID NLM: 101699846
Informations de publication
Date de publication:
12 Jul 2023
12 Jul 2023
Historique:
received:
07
02
2023
accepted:
27
06
2023
medline:
13
7
2023
pubmed:
13
7
2023
entrez:
12
7
2023
Statut:
epublish
Résumé
Extensive tracheal injury or disease can be life-threatening but there is currently no standard of care. Regenerative medicine offers a potential solution to long-segment tracheal defects through the creation of scaffolds that support the generation of healthy neotissue. We developed decellularized tracheal grafts (PDTG) by removing the cells of the epithelium and lamina propria while preserving donor cartilage. We previously demonstrated that PDTG support regeneration of host-derived neotissue. Here, we use a combination of microsurgical, immunofluorescent, and transcriptomic approaches to compare PDTG neotissue with the native airway and surgical controls. We report that PDTG neotissue is composed of native tracheal cell types and that the neoepithelium and microvasculature persisted for at least 6 months. Vascular perfusion of PDTG was established within 2 weeks and the graft recruited multipotential airway stem cells that exhibit normal proliferation and differentiation. Hence, PDTG neotissue recapitulates the structure and function of the host trachea and has the potential to regenerate.
Identifiants
pubmed: 37438368
doi: 10.1038/s41536-023-00312-4
pii: 10.1038/s41536-023-00312-4
pmc: PMC10338482
doi:
Types de publication
Journal Article
Langues
eng
Pagination
35Subventions
Organisme : NHLBI NIH HHS
ID : K08 HL138460
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL157039
Pays : United States
Informations de copyright
© 2023. The Author(s).
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