Hydrogen peroxide from L-amino acid oxidase of king cobra (Ophiophagus hannah) venom attenuates Pseudomonas biofilms.

Animals Pseudomonas L-Amino Acid Oxidase / pharmacology Hydrogen Peroxide Ophiophagus hannah Pseudomonas aeruginosa Pseudomonas Infections / drug therapy

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
12 07 2023
Historique:
received: 03 03 2023
accepted: 29 06 2023
medline: 14 7 2023
pubmed: 13 7 2023
entrez: 12 7 2023
Statut: epublish

Résumé

Because of the high incidence of Pseudomonas aeruginosa biofilms-related nosocomial infections, venoms from common Thai snakes were tested. Although venoms from king cobra (Ophiophagus hannah; OH) and green pit viper (Trimeresurus albolabris) showed the broadest antibacterial spectrum, OH venom demonstrated more profound anti-biofilm activities against P. aeruginosa. Additionally, purified L-amino acid oxidase from OH venom (OH-LAAO), using a three-step chromatography and protein identification, reduced biofilm mass as indicated by the downregulation of several genes, including the genes for biofilm synthesis (algD and pslB) and biofilm regulators (algU, gacA, and siaD). Moreover, OH-LAAO disrupted Pseudomonas-preformed biofilms via upregulation of several genes for biofilm dispersion (nbdA, bdlA, and dipA) and biofilm degradation (endA and pslG), resulting in a reduction of the biofilm biomass. Due to the antimicrobial effects and anti-biofilm activities (reduced production plus increased dispersion) neutralized by catalase, a hydrogen peroxide (H

Identifiants

DOI: 10.1038/s41598-023-37914-3 PMID: 37438396 PMC: PMC10338509
pubmed: 37438396
doi: 10.1038/s41598-023-37914-3
pii: 10.1038/s41598-023-37914-3
pmc: PMC10338509
doi:

Substances chimiques

L-Amino Acid Oxidase EC 1.4.3.2
Hydrogen Peroxide BBX060AN9V

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

11304

Informations de copyright

© 2023. The Author(s).

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Auteurs

Uthaibhorn Singkham-In (U)

Department of Microbiology, Faculty of Medicine, Chulalongkorn University, 1873 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand.
Center of Excellence in Translational Research in Inflammatory and Immunology (CETRII), Faculty of Medicine, Chulalongkorn University, 1873 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand.

Wichit Thaveekarn (W)

Queen Saovabha Memorial Institute, Thai Red Cross Society, 1871 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand.

Jureeporn Noiphrom (J)

Queen Saovabha Memorial Institute, Thai Red Cross Society, 1871 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand.

Orawan Khow (O)

Queen Saovabha Memorial Institute, Thai Red Cross Society, 1871 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand.

Surada Ponwaranon (S)

Department of Microbiology, Faculty of Medicine, Chulalongkorn University, 1873 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand.
Center of Excellence in Translational Research in Inflammatory and Immunology (CETRII), Faculty of Medicine, Chulalongkorn University, 1873 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand.

Jiraphorn Issara-Amphorn (J)

Functional Cellular Networks Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, 20892, USA.

Visith Sitprija (V)

Queen Saovabha Memorial Institute, Thai Red Cross Society, 1871 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand.

Asada Leelahavanichkul (A)

Department of Microbiology, Faculty of Medicine, Chulalongkorn University, 1873 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand. aleelahavanit@gmail.com.
Center of Excellence in Translational Research in Inflammatory and Immunology (CETRII), Faculty of Medicine, Chulalongkorn University, 1873 Rama 4 Road, Pathumwan, Bangkok, 10330, Thailand. aleelahavanit@gmail.com.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Hydrogen peroxide from L-amino acid oxidase of...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Pseudomonadaceae › Pseudomonas › Hydrogen peroxide from L-amino acid oxidase of...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Oxidoreductases acting on CH-NH2 group donors › Amino-acid oxidoreductases › L-Amino acid oxidase › Hydrogen peroxide from L-amino acid oxidase of...
questionsmedicales.fr › Composés chimiques organiques › Radicaux libres › Peroxydes › Peroxyde d'hydrogène › Hydrogen peroxide from L-amino acid oxidase of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Reptiles › Serpents › Alethinophidia › Elapidae › Ophiophagus hannah › Hydrogen peroxide from L-amino acid oxidase of...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa › Hydrogen peroxide from L-amino acid oxidase of...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram négatif › Infections à Pseudomonas › Hydrogen peroxide from L-amino acid oxidase of...