An integrated strategy by absorbed component characterization, pharmacokinetics, and activity evaluation for identification of potential nephroprotective substances in Zhu-Ling decoction.
Zhu-Ling decoction
absorbed components characterization
effective substances
nephroprotective activity
pharmacokinetics
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
revised:
26
06
2023
received:
10
05
2023
accepted:
28
06
2023
medline:
6
9
2023
pubmed:
13
7
2023
entrez:
13
7
2023
Statut:
ppublish
Résumé
An efficient strategy for the identification of potential nephroprotective substances in Zhu-Ling decoction has been established with the integration of absorbed components characterization, pharmacokinetics, and activity evaluation. A qualitative method was developed to characterize the chemical constituents absorbed components in vivo of Zhu-Ling decoction by using ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. A quantitative method was established and validated for the simultaneous determination of eight compounds in rat plasma by using ultra-performance liquid chromatography-triple quadruple tandem mass spectrometry. Finally, the nephroprotective activities of absorbed components with high exposure were assessed by cell survival rate, superoxide dismutase, and malondialdehyde activities in hydrogen peroxide-induced Vero cells. As a result, 111 compounds in Zhu-Ling decoction and 36 absorbed components were identified in rat plasma and urine, and poricoic acid A, poricoic acid B, alisol A, 16-oxo-alisol A, and dehydro-tumulosic acid had high exposure levels in rat plasma. Finally, poricoic acid B, poricoic acid A, 16-oxo-alisol A, and dehydro-tumulosic acid showed remarkable nephroprotective activity against Vero cells damage induced by hydrogen peroxide. Besides, superoxide dismutase and malondialdehyde activities were obviously regulated in hydrogen peroxide-induced Vero cells by treatment with the four compounds mentioned above. Therefore, these four compounds were considered to be effective substances of Zhu-Ling decoction due to their relatively high exposure in vivo and biological activity. This study provided a chemical basis for the action mechanism of Zhu-Ling decoction in the treatment of chronic kidney diseases.
Identifiants
pubmed: 37438987
doi: 10.1002/jssc.202300331
doi:
Substances chimiques
poricoic acid A
0
Hydrogen Peroxide
BBX060AN9V
Triterpenes
0
Drugs, Chinese Herbal
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300331Informations de copyright
© 2023 Wiley-VCH GmbH.
Références
Chen YH, Fan LH, Zhang T, Liu XQ. Effectiveness of Zhuling decoction on diuretic resistance in with heart failure: a controlled trial. J Tradit Chin Med. 2022;42:439-45.
Wang YT, Kong LM, Cheng J, Zhang Y, Wang CY, Wang ZC, et al. Study on quality evaluation method of classical prescription Zhuling decoction primary standard substances. Chin Tradit Herb Drugs. 2022;53:3643-52.
Zhang SS, Wang X, Wang T, Zhang LF. The meta-analysis for Zhuling decoction to treat the chronic glomerulonephritis. World Chin Med. 2020;15:1306-11.
Zhuang XY, Lv J. Research progress of Zhuling decoction in the treatment of renal diseases. J Chin Prescrip Drug. 2022;20:152-3.
Kim B, Jo HG. Adjuvant therapy efficacy of herbal medicine Zeo Lyung Tang (Zhu Ling Decoction) for primary glomerulonephritis: systematic review and meta-analysis, Korean J Int Med. 2020;41:644-57.
Huang, KC, Su YC, Sun MF, Huang ST. Chinese herbal medicine improves the long-term survival rate of patients with chronic kidney disease in Taiwan: a nationwide retrospective population-based cohort study, Front Pharmacol. 2018;9:1117.
Tsai CH, Pan TC, Lai MT, Lee SC, Chen ML, Jheng JR, et al. Prophylaxis of experimentally induced calcium oxalate nephrolithiasis in rats by Zhulingtang, a traditional Chinese herbal formula, Urol Int. 2009;82:464-71.
He D, Ren YF, Hua X, Zhang J, Zhang B, Dong J, et al. Phytochemistry and bioactivities of the main constituents of Polyporus umbellatus (Pers.) Fries, Phytomedicine. 2022;103:154196.
Zou YT, Long F, Wu CY, Zhou J, Zhang W, Xu JD, et al. A dereplication strategy for identifying triterpene acid analogues in Poria cocos by comparing predicted and acquired UPLC-ESI-QTOF-MS/MS data, Phytochem Anal. 2019;30:292-310.
Lu J, Tian J, Zhou L, Meng LJ, Chen ST, Ma CY, et al. Phytochemistry and biological activities of Poria, J Chem. 2021;2021:6659775.
Zhang LL, Xu W, Xu YL, Chen XP, Huang MQ, Lu JJ. Therapeutic potential of Rhizoma Alismatis: a review on ethnomedicinal application, phytochemistry, pharmacology, and toxicology, Ann N Y Acad Sci. 2017;1401:90-101.
Wang DL, Ru WW, Xu YP, Zhang JL, He XX, Fan GH, et al. Chemical constituents and bioactivities of Colla corii asini, Drug Discov Ther. 2014;8:201-7.
Zhou X, Li CG, Chang D, Bensoussan A. Current status and major challenges to the safety and efficacy presented by Chinese herbal medicine, Medicines. 2019;6:14.
Zhang Y, Zhu MD, Zhang FG, Zhang SQ, Du WX, Xiao XF. Integrating pharmacokinetics study, network analysis, and experimental validation to uncover the mechanism of Qiliqiangxin capsule against chronic heart failure, Front Pharmacol. 2019;10:1046.
Tang XY, Dai ZQ, Zeng JX, Li ZT, Fan CL, Yao ZH, et al. Pharmacokinetics, hepatic disposition, and heart tissue distribution of 14 compounds in rat after oral administration of Qi-Li-Qiang-Xin capsule via ultra-high-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry, J Sep Sci. 2022;45:2177-89.
Zhao WL, Huang XQ, Li XY, Zhang FF, Chen SN, Ye M, et al. Qualitative and quantitative analysis of major triterpenoids in Alismatis Rhizoma by high performance liquid chromatography/diode-array detector/quadrupole time-of-flight mass spectrometry and ultra-performance liquid chromatography/triple quadrupole mass spectrometry, Molecules. 2015;20:13958-81.
Zhu LX, Xu J, Zhang SJ, Wang RJ, Huang Q, Chen HB, et al. Qualitatively and quantitatively comparing secondary metabolites in three medicinal parts derived from Poria cocos (Schw.) Wolf using UHPLC-QTOF-MS/MS-based chemical profiling, J Pharm Biomed Anal. 2018;150:278-86.
Feng GF, Zheng Y, Sun YF, Liu S, Pi ZF, Song FR, et al. A targeted strategy for analyzing untargeted mass spectral data to identify lanostane-type triterpene acids in Poria cocos by integrating a scientific information system and liquid chromatography-tandem mass spectrometry combined with ion mobility spectrometry, Anal Chim Acta. 2018;1033:87-99.
Tai YN, Zou FX, Zhang QR, Wang J, Rao RH, Xie RH, et al. Quantitative analysis of eight triterpenoids and two sesquiterpenoids in Rhizoma Alismatis by using UPLC-ESI/APCI-MS/MS and its application to optimisation of best harvest time and crude processing temperature, J Anal Methods Chem. 2019;2019:8320171.
Wang L, Li S, Li JX, Cheng ZZ, Feng YL, Ouyang H, et al. Comprehensive metabolic profiling of Alismatis Rhizoma triterpenes in rats based on characteristic ions and a triterpene database, J Pharm Anal. 2021;11:96-107.
Feng GF, Li SZ, Liu S, Song FR, Pi ZF, Liu ZQ. Targeted screening approach to systematically identify the absorbed effect substances of Poria cocos in vivo using ultrahigh performance liquid chromatography tandem mass spectrometry, J Agric Food Chem. 2018;66:8319-27.
Cheng ZH, Ding CG, Li Z, Song DZ, Yuan J, Hao WS, et al. Simultaneous determination of three triterpenes in rat plasma by LC-MS/MS and its application to a pharmacokinetic study of Rhizoma Alismatis extract. J Chromatogr B. 2016;1006:32-7.
Xiao F, Li Q, Liang K, Zhao LS, He BS, Ji W, et al. Comparative pharmacokinetics of three triterpene acids in rat plasma after oral administration of Poria extract and its formulated herbal preparation: GuiZhi-FuLing capsule, Fitoterapia. 2016;83:117-24.
Trivedi V, Upadhyay V, Yadav M, Shrivastav PS, Sanyal M. Impact of electrospray ion source platforms on matrix effect due to plasma phospholipids in the determination of rivastigmine by LC-MS/MS, Bioanalysis. 2014;6:2301-6.
Chen DQ, Wu XQ, Chen L, Hu HH, Wang YH, Zhang YY. Poricoic acid A as a modulator of TPH-1 expression inhibits renal fibrosisviamodulating protein stability of beta-catenin and beta-catenin-mediated transcription, Ther Adv Chronic Dis. 2020;11:2040622320962648.
Luan ZL, Ming WH, Sun XW, Zhang C, Zhou Y, Zheng F, et al. A naturally occurring FXR agonist, alisol B 23-acetate, protects against renal ischemia-reperfusion injury, Am J Physiol Renal Physiol. 2021, 321, F617-28.
Guo N, Bai ZL, Jia WJ, Sun JH, Wang WW, Chen SZ, et al. Quantitative analysis of polysaccharide composition in Polyporus umbellatus by HPLC-ESI-TOF-MS, Molecules. 2019;24:2526.
Huang HY, Liu S, Du JY, Lin JH, Liang QT, Liu ST, et al. Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry, Glycoconj J. 2020;37:201-7.
Chen Y, Yao FK, Ming K, Wang DY, Hu YL, Liu JG. Polysaccharides from traditional Chinese medicines: extraction, purification, modification, and biological activity, Molecules. 2016;21:1705.
Sun YC. Biological activities and potential health benefits of polysaccharides from Poria cocos and their derivatives, Int J Biol Macromol. 2014;68:131-4.