Identification of Pathways for Production of D-Glucaric Acid by Pseudogluconobacter saccharoketogenes.
Alcohol dehydrogenase
Aldehyde dehydrogenase
Glucaric acid
Glucuronic acid
Pseudogluconobacter saccharoketogenes
Journal
Applied biochemistry and biotechnology
ISSN: 1559-0291
Titre abrégé: Appl Biochem Biotechnol
Pays: United States
ID NLM: 8208561
Informations de publication
Date de publication:
13 Jul 2023
13 Jul 2023
Historique:
accepted:
01
07
2023
medline:
13
7
2023
pubmed:
13
7
2023
entrez:
13
7
2023
Statut:
aheadofprint
Résumé
Pseudogluconobacter saccharoketogenes produces glucaric acid from D-glucose via two pathways, i.e., through D-glucuronic acid or D-gluconic acid. These pathways are catalyzed by alcohol dehydrogenase, aldehyde dehydrogenase, and gluconate dehydrogenase. Although D-glucaraldehyde and L-guluronic acid are also theorized to be produced in pathways throsugh D-glucuronic acid and D-gluconic acid, respectively, no direct data to identify these intermediates have been reported. In this study, the intermediates were purified and identified as D-glucaraldehyde and L-guluronic acid. The substrate specificities of the three enzymes on these intermediates and their oxidation products were studied, and the roles of alcohol, aldehyde, and gluconate dehydrogenases in D-glucaric acid-producing pathways were elucidated using the intermediates. Additionally, the substrate specificities of alcohol and aldehyde dehydrogenases on some alcohols, aldehydes, and aldoses were determined. Alcohol dehydrogenase showed wide substrate specificities, whereas the substrates oxidized by aldehyde dehydrogenase were limited. A 30-L scale reaction using the resting cells of Rh47-3 revealed that D-glucaric acid was produced from D-glucose and D-gluconic acid in 60.3 mol% (7.0 g/L) and 78.6 mol% (22.5 g/L) yields, respectively.
Identifiants
pubmed: 37440113
doi: 10.1007/s12010-023-04628-1
pii: 10.1007/s12010-023-04628-1
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Society for the Promotion of Science
ID : 15K06593
Organisme : Japan Society for the Promotion of Science
ID : 20K05821
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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