Testing Antimicrobial Properties of Human Lactoferrin-Derived Fragments.
antimicrobial peptides
bacteria
lactoferrin
non-antimicrobial peptides
prediction
protease inhibitor
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
23 Jun 2023
23 Jun 2023
Historique:
received:
28
05
2023
revised:
19
06
2023
accepted:
20
06
2023
medline:
17
7
2023
pubmed:
14
7
2023
entrez:
14
7
2023
Statut:
epublish
Résumé
Lactoferrin, an iron-binding glycoprotein, plays a significant role in the innate immune system, with antibacterial, antivirial, antifungal, anticancer, antioxidant and immunomodulatory functions reported. It is worth emphasizing that not only the whole protein but also its derived fragments possess antimicrobial peptide (AMP) activity. Using AmpGram, a top-performing AMP classifier, we generated three novel human lactoferrin (hLF) fragments: hLF 397-412, hLF 448-464 and hLF 668-683, predicted with high probability as AMPs. For comparative studies, we included hLF 1-11, previously confirmed to kill some bacteria. With the four peptides, we treated three Gram-negative and three Gram-positive bacterial strains. Our results indicate that none of the three new lactoferrin fragments have antimicrobial properties for the bacteria tested, but hLF 1-11 was lethal against
Identifiants
pubmed: 37445717
pii: ijms241310529
doi: 10.3390/ijms241310529
pmc: PMC10342102
pii:
doi:
Substances chimiques
Lactoferrin
EC 3.4.21.-
Peptides
0
Antifungal Agents
0
Anti-Bacterial Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Science Center
ID : 2017/26/D/NZ8/00444
Organisme : National Science Center
ID : 2019/35/N/NZ8/03366
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