Inhibitory Investigations of Acyl-CoA Derivatives against Human Lipoxygenase Isozymes.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
30 Jun 2023
Historique:
received: 05 06 2023
revised: 26 06 2023
accepted: 27 06 2023
medline: 17 7 2023
pubmed: 14 7 2023
entrez: 14 7 2023
Statut: epublish

Résumé

Lipid metabolism is a complex process crucial for energy production resulting in high levels of acyl-coenzyme A (acyl-CoA) molecules in the cell. Acyl-CoAs have also been implicated in inflammation, which could be possibly linked to lipoxygenase (LOX) biochemistry by the observation that an acyl-CoA was bound to human platelet 12-lipoxygenase via cryo-EM. Given that LOX isozymes play a pivotal role in inflammation, a more thorough investigation of the inhibitory effects of acyl-CoAs on lipoxygenase isozymes was judged to be warranted. Subsequently, it was determined that C18 acyl-CoA derivatives were the most potent against h12-LOX, human reticulocyte 15-LOX-1 (h15-LOX-1), and human endothelial 15-LOX-2 (h15-LOX-2), while C16 acyl-CoAs were more potent against human 5-LOX. Specifically, oleoyl-CoA (18:1) was most potent against h12-LOX (IC

Identifiants

pubmed: 37446119
pii: ijms241310941
doi: 10.3390/ijms241310941
pmc: PMC10341549
pii:
doi:

Substances chimiques

Lipoxygenase EC 1.13.11.12
Isoenzymes 0
Oxylipins 0
Acyl Coenzyme A 0
Scavenger Receptors, Class E 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIGMS NIH HHS
ID : gm131835
Pays : United States

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Auteurs

Michelle Tran (M)

Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, CA 95064, USA.

Kevin Yang (K)

Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, CA 95064, USA.

Alisa Glukhova (A)

Department of Biochemistry and Pharmacology, University of Melbourne, Melbourne, VIC 3010, Australia.
Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia.
Department of Medical Biology, University of Melbourne, Melbourne, VIC 3010, Australia.
Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC 3052, Australia.
ARC Centre for Cryo-Electron Microscopy of Membrane Proteins, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC 3052, Australia.

Michael Holinstat (M)

Department of Pharmacology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Theodore Holman (T)

Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, CA 95064, USA.

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Classifications MeSH