Phase Separation of DNA-Encoded Artificial Cells Boosts Signal Amplification for Biosensing.
Aptamers
Artificial Cell
Biosensing
Liquid-Liquid Separation
Signal Amplification
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
25 09 2023
25 09 2023
Historique:
received:
12
05
2023
medline:
19
9
2023
pubmed:
17
7
2023
entrez:
16
7
2023
Statut:
ppublish
Résumé
Life-like hierarchical architecture shows great potential for advancing intelligent biosensing, but modular expansion of its sensitivity and functionality remains a challenge. Drawing inspiration from intracellular liquid-liquid phase separation, we discovered that a DNA-encoded artificial cell with a liquid core (LAC) can enhance peroxidase-like activity of Hemin and its DNA G-quadruplex aptamer complex (DGAH) without substrate-selectivity, unlike its gelled core (GAC) counterpart. The LAC is easily engineered as an ultrasensitive biosensing system, benefiting from DNA's high programmability and unique signal amplification capability mediated by liquid-liquid phase separation. As proof of concept, its versatility was successfully demonstrated by coupling with two molecular recognition elements to monitor tumor-related microRNA and profile cancer cell phenotypes. This scalable design philosophy offers new insights into the design of next generation of artificial cells-based biosensors.
Identifiants
pubmed: 37455257
doi: 10.1002/anie.202306691
doi:
Substances chimiques
DNA
9007-49-2
MicroRNAs
0
Hemin
743LRP9S7N
DNA, Catalytic
0
Aptamers, Nucleotide
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202306691Informations de copyright
© 2023 Wiley-VCH GmbH.
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