Continuous vs. discontinuous purification of isolated human islets: functional and morphological comparison.
COBE 2991 cell processor
continuous density gradient
discontinuous density gradient
islet isolation
islet purification
Journal
Frontiers in endocrinology
ISSN: 1664-2392
Titre abrégé: Front Endocrinol (Lausanne)
Pays: Switzerland
ID NLM: 101555782
Informations de publication
Date de publication:
2023
2023
Historique:
received:
28
03
2023
accepted:
09
06
2023
medline:
18
7
2023
pubmed:
17
7
2023
entrez:
17
7
2023
Statut:
epublish
Résumé
The COBE 2991 cell processor, commonly used for pancreatic islet isolation, is no longer distributed in Europe, leading to a search for alternative purification procedures with equivalent efficacy. The aim of this study was to evaluate the efficacy of an alternative method based on the discontinuous purification of islets. The conventional isolation procedure using a standard continuous islet purification with COBE 2991 of A similar islet yield was obtained using continuous vs. discontinuous purification methods (76,292.5 ± 40,550.44 vs. 79,625 ± 41,484.46 islet equivalents, Compared to the conventional purification procedure, discontinuous purification with a bottle method shows similar results with regard to isolation yield and islet secretory function. Furthermore, this alternative technique allows for obtaining larger islets.
Sections du résumé
Background
The COBE 2991 cell processor, commonly used for pancreatic islet isolation, is no longer distributed in Europe, leading to a search for alternative purification procedures with equivalent efficacy. The aim of this study was to evaluate the efficacy of an alternative method based on the discontinuous purification of islets.
Methods
The conventional isolation procedure using a standard continuous islet purification with COBE 2991 of
Results
A similar islet yield was obtained using continuous vs. discontinuous purification methods (76,292.5 ± 40,550.44 vs. 79,625 ± 41,484.46 islet equivalents,
Conclusion
Compared to the conventional purification procedure, discontinuous purification with a bottle method shows similar results with regard to isolation yield and islet secretory function. Furthermore, this alternative technique allows for obtaining larger islets.
Identifiants
pubmed: 37455917
doi: 10.3389/fendo.2023.1195545
pmc: PMC10348810
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1195545Informations de copyright
Copyright © 2023 Buemi, Mouard, Darius, Devresse, Kanaan, Gianello and Mourad.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Surgery. 1987 Nov;102(5):869-79
pubmed: 3313779
Diabetes. 2007 Mar;56(3):594-603
pubmed: 17327426
Am J Physiol Endocrinol Metab. 2015 Oct 1;309(7):E640-50
pubmed: 26264556
Cell Transplant. 2017 May 9;26(5):901-911
pubmed: 27938490
Transplantation. 2018 Nov;102(11):1857-1863
pubmed: 30063695
Cell Transplant. 2016;25(8):1515-1523
pubmed: 26922947
Diabetes. 1988 Apr;37(4):413-20
pubmed: 3288530
J Clin Med. 2020 Dec 23;10(1):
pubmed: 33374512
Diabetes Care. 2022 Oct 17;:
pubmed: 36250905
Adv Exp Med Biol. 2010;654:683-710
pubmed: 20217520
N Engl J Med. 2000 Jul 27;343(4):230-8
pubmed: 10911004
Diabetes. 1989 Jan;38 Suppl 1:143-5
pubmed: 2642839