Alteration of extracellular matrix proteins in atrophic periodontal ligament of hypofunctional rat molars.
Journal
BDJ open
ISSN: 2056-807X
Titre abrégé: BDJ Open
Pays: England
ID NLM: 101709456
Informations de publication
Date de publication:
18 Jul 2023
18 Jul 2023
Historique:
received:
07
04
2023
accepted:
19
06
2023
revised:
17
06
2023
medline:
19
7
2023
pubmed:
19
7
2023
entrez:
18
7
2023
Statut:
epublish
Résumé
The aim of this study was to investigate the effect of mechanical force on possible dynamic changes of the matrix proteins deposition in the PDL upon in vitro mechanical and in vivo occlusal forces in a rat model with hypofunctional conditions. Intermittent compressive force (ICF) and shear force (SF) were applied to human periodontal ligament stem cells (PDLSCs). Protein expression of collagen I and POSTN was analyzed by western blot technique. To establish an in vivo model, rat maxillary molars were extracted to facilitate hypofunction of the periodontal ligament (PDL) tissue of the opposing mandibular molar. The mandibles were collected after 4-, 8-, and 12-weeks post-extraction and used for micro-CT and immunohistochemical analysis. ICF and SF increased the synthesis of POSTN by human PDLSCs. Histological changes in the hypofunctional teeth revealed a narrowing of the PDL space, along with a decreased amount of collagen I, POSTN, and laminin in perivascular structures compared to the functional contralateral molars. Our results revealed that loss of occlusal force disrupts deposition of some major matrix proteins in the PDL, underscoring the relevance of mechanical forces in maintaining periodontal tissue homeostasis by modulating ECM composition.
Identifiants
pubmed: 37463885
doi: 10.1038/s41405-023-00155-7
pii: 10.1038/s41405-023-00155-7
pmc: PMC10353993
doi:
Types de publication
Journal Article
Langues
eng
Pagination
31Informations de copyright
© 2023. The Author(s).
Références
Arch Oral Biol. 1965 Nov-Dec;10(6):909-19
pubmed: 5226995
J Physiol. 2005 Sep 15;567(Pt 3):1021-33
pubmed: 16002437
J Periodontol. 2018 Jun;89 Suppl 1:S214-S222
pubmed: 29926937
Int J Cell Biol. 2015;2015:369874
pubmed: 26106417
Anat Rec A Discov Mol Cell Evol Biol. 2004 Dec;281(2):1227-33
pubmed: 15532025
J Cell Sci. 2007 Aug 1;120(Pt 15):2672-82
pubmed: 17635998
Mol Cell Biol. 2005 Dec;25(24):11131-44
pubmed: 16314533
Nat Mater. 2009 Mar;8(3):175-88
pubmed: 19229265
Dev Biol. 2004 Mar 15;267(2):473-87
pubmed: 15013807
J Bone Miner Res. 1999 Jul;14(7):1239-49
pubmed: 10404027
Exp Ther Med. 2016 May;11(5):1812-1818
pubmed: 27168809
Angle Orthod. 2016 Mar;86(2):214-20
pubmed: 25989212
Nature. 2001 Nov 1;414(6859):98-104
pubmed: 11689954
J Physiol. 1999 Mar 15;515 ( Pt 3):919-27
pubmed: 10066916
Am J Anat. 1990 Jan;187(1):91-7
pubmed: 2296913
Wound Repair Regen. 2000 May-Jun;8(3):238-46
pubmed: 10886814
Wound Repair Regen. 2000 May-Jun;8(3):226-37
pubmed: 10886813
J Dent Res. 2003 Jun;82(6):486-90
pubmed: 12766204
Anat Rec A Discov Mol Cell Evol Biol. 2004 Dec;281(2):1264-75
pubmed: 15386274
Adv Exp Med Biol. 2014;802:31-47
pubmed: 24443019
Circ Res. 2002 Nov 1;91(9):769-75
pubmed: 12411390
Cell Death Dis. 2019 Oct 7;10(10):761
pubmed: 31591384
Nat Commun. 2015 Aug 14;6:8026
pubmed: 26272817
Biochim Biophys Acta. 2014 Aug;1840(8):2506-19
pubmed: 24418517
J Biol Chem. 1979 Oct 10;254(19):9933-7
pubmed: 114518
Cell Mol Life Sci. 2011 Oct;68(19):3201-7
pubmed: 21833583
Angle Orthod. 2013 Jan;83(1):48-56
pubmed: 22716278
J Biol Chem. 2009 Dec 18;284(51):35939-50
pubmed: 19837663
J Periodontol. 1980 Jul;51(7):393-9
pubmed: 6930479
Int J Mol Sci. 2022 Jun 27;23(13):
pubmed: 35806124
J Endod. 2022 Jul;48(7):893-901
pubmed: 35405160
J Cell Biochem. 2007 Jun 1;101(3):695-711
pubmed: 17226767
J Cell Sci. 2010 Dec 15;123(Pt 24):4195-200
pubmed: 21123617
Malays J Med Sci. 2017 Oct;24(5):101-105
pubmed: 29386977
J Indian Soc Periodontol. 2018 Sep-Oct;22(5):395-400
pubmed: 30210187
J Periodontol. 2007 Jun;78(6):1113-9
pubmed: 17539726
J Dent Res. 2015 Jul;94(7):983-9
pubmed: 25870205
J Cell Physiol. 1995 Apr;163(1):179-93
pubmed: 7534769
J Periodontal Res. 2001 Feb;36(1):9-17
pubmed: 11246706
Acta Odontol Scand. 2016 Oct;74(7):518-524
pubmed: 27462822