The Search for Single-Domain Antibodies Interacting with the Receptor-Binding Domain of SARS-CoV-2 Surface Protein.
SARS-CoV-2
affinity selection
bacteriophage
phage library
single-domain antibodies
Journal
Bulletin of experimental biology and medicine
ISSN: 1573-8221
Titre abrégé: Bull Exp Biol Med
Pays: United States
ID NLM: 0372557
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
received:
08
11
2022
medline:
28
7
2023
pubmed:
19
7
2023
entrez:
18
7
2023
Statut:
ppublish
Résumé
We performed a search for nanoantibodies that specifically interact with the receptor-binding domain (RBD) of the SARS-CoV-2 surface protein. The specificity of single-domain antibodies from the blood sera of a llama immunized with RBD of SARS-CoV-2 surface protein S (variant B.1.1.7 (Alpha)) was analyzed by ELISA. Recombinant trimers of the SARS-CoV-2 spike protein were used as antigens. In this work, a set of single-domain antibodies was obtained that specifically bind to the RBD of the SARS-CoV-2 virus.
Identifiants
pubmed: 37464199
doi: 10.1007/s10517-023-05839-6
pii: 10.1007/s10517-023-05839-6
doi:
Substances chimiques
spike protein, SARS-CoV-2
0
Single-Domain Antibodies
0
Antibodies, Neutralizing
0
Antibodies, Viral
0
Membrane Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
225-228Informations de copyright
© 2023. Springer Science+Business Media, LLC, part of Springer Nature.
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