Bifidobacterium adolescentis induces Decorin
B.adolescentis
Colorectal cancer
Decorin
Macrophages
TLR2
Journal
Journal of experimental & clinical cancer research : CR
ISSN: 1756-9966
Titre abrégé: J Exp Clin Cancer Res
Pays: England
ID NLM: 8308647
Informations de publication
Date de publication:
18 Jul 2023
18 Jul 2023
Historique:
received:
07
03
2023
accepted:
30
06
2023
medline:
21
7
2023
pubmed:
19
7
2023
entrez:
18
7
2023
Statut:
epublish
Résumé
The interplay between gut microbiota and tumor microenvironment (TME) in the pathogenesis of colorectal cancer (CRC) is largely unknown. Here, we elucidated the functional role of B. adolescentis and its possible mechanism on the manipulation of Decorin The relative abundance of B. adolescentis in tumor or para-tumor tissue of CRC patients was analyzed. The role of B. adolescentis was explored in the CRC animal models. The single cell-RNA sequencing (scRNA-seq) was used to investigate the myeloid cells subsets in TME. The expression level of TLR2/YAP axis and its downstream Decorin in macrophages were tested by Western blot and qRT-PCR. Knockdown of Decorin in Raw264.7 was performed to investigate the effect of Decorin We found that the abundance of B. adolescentis was significantly reduced in tumor tissue of CRC patients. Supplementation with B. adolescentis suppressed AOM/DSS-induced tumorigenesis in mice. ScRNA-seq and animal experiment revealed that B. adolescentis increased Decorin These results highlight that B. adolescentis induced Decorin
Sections du résumé
BACKGROUND
BACKGROUND
The interplay between gut microbiota and tumor microenvironment (TME) in the pathogenesis of colorectal cancer (CRC) is largely unknown. Here, we elucidated the functional role of B. adolescentis and its possible mechanism on the manipulation of Decorin
METHODS
METHODS
The relative abundance of B. adolescentis in tumor or para-tumor tissue of CRC patients was analyzed. The role of B. adolescentis was explored in the CRC animal models. The single cell-RNA sequencing (scRNA-seq) was used to investigate the myeloid cells subsets in TME. The expression level of TLR2/YAP axis and its downstream Decorin in macrophages were tested by Western blot and qRT-PCR. Knockdown of Decorin in Raw264.7 was performed to investigate the effect of Decorin
RESULTS
RESULTS
We found that the abundance of B. adolescentis was significantly reduced in tumor tissue of CRC patients. Supplementation with B. adolescentis suppressed AOM/DSS-induced tumorigenesis in mice. ScRNA-seq and animal experiment revealed that B. adolescentis increased Decorin
CONCLUSIONS
CONCLUSIONS
These results highlight that B. adolescentis induced Decorin
Identifiants
pubmed: 37464382
doi: 10.1186/s13046-023-02746-6
pii: 10.1186/s13046-023-02746-6
pmc: PMC10353206
doi:
Substances chimiques
Decorin
0
Toll-Like Receptor 2
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
172Subventions
Organisme : National Natural Science Foundation of China
ID : 82072623
Organisme : National Natural Science Foundation of China
ID : 82270573
Organisme : National Natural Science Foundation of China
ID : 82203618
Organisme : Zhejiang Province Natural Science Foundation
ID : LZ22H160002
Organisme : Zhejiang Provincial Medical and Health Science and Technology Program
ID : 2023KY722
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2023. The Author(s).
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