Immunogenomic profile at baseline predicts host susceptibility to clinical malaria.
RNA sequencing
baseline immunity
human parasite immunology
immunoinformatic analysis
malaria immunity
malaria susceptibility
mass cytometrý
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2023
2023
Historique:
received:
03
03
2023
accepted:
19
06
2023
medline:
21
7
2023
pubmed:
19
7
2023
entrez:
19
7
2023
Statut:
epublish
Résumé
Host gene and protein expression impact susceptibility to clinical malaria, but the balance of immune cell populations, cytokines and genes that contributes to protection, remains incompletely understood. Little is known about the determinants of host susceptibility to clinical malaria at a time when acquired immunity is developing. We analyzed peripheral blood mononuclear cells (PBMCs) collected from children who differed in susceptibility to clinical malaria, all from a small town in Mali. PBMCs were collected from children aged 4-6 years at the start, peak and end of the malaria season. We characterized the immune cell composition and cytokine secretion for a subset of 20 children per timepoint (10 children with no symptomatic malaria age-matched to 10 children with >2 symptomatic malarial illnesses), and gene expression patterns for six children (three per cohort) per timepoint. We observed differences between the two groups of children in the expression of genes related to cell death and inflammation; in particular, inflammatory genes such as CXCL10 and STAT1 and apoptotic genes such as XAF1 were upregulated in susceptible children before the transmission season began. We also noted higher frequency of HLA-DR+ CD4 T cells in protected children during the peak of the malaria season and comparable levels cytokine secretion after stimulation with malaria schizonts across all three time points. This study highlights the importance of baseline immune signatures in determining disease outcome. Our data suggests that differences in apoptotic and inflammatory gene expression patterns can serve as predictive markers of susceptibility to clinical malaria.
Identifiants
pubmed: 37465667
doi: 10.3389/fimmu.2023.1179314
pmc: PMC10351378
doi:
Substances chimiques
Cytokines
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1179314Subventions
Organisme : FIC NIH HHS
ID : D43 TW001589
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI110820
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI065683
Pays : United States
Organisme : NIAID NIH HHS
ID : N01AI85346
Pays : United States
Informations de copyright
Copyright © 2023 Mbambo, Dwivedi, Ifeonu, Munro, Shrestha, Bromley, Hodges, Adkins, Kouriba, Diarra, Niangaly, Kone, Coulibaly, Traore, Dolo, Thera, Laurens, Doumbo, Plowe, Berry, Travassos, Lyke and Silva.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
F1000Res. 2020 Oct 22;9:1263
pubmed: 36072920
J Immunol Methods. 2004 Oct;293(1-2):127-42
pubmed: 15541283
Nat Med. 2004 Jan;10(1):29-30
pubmed: 14702631
J Immunol. 2021 Jul 15;207(2):523-533
pubmed: 34193602
BMC Bioinformatics. 2009 Apr 09;10:106
pubmed: 19358741
Cell. 2014 Apr 10;157(2):499-513
pubmed: 24725414
Infect Immun. 2012 Mar;80(3):1150-5
pubmed: 22232187
Nat Rev Immunol. 2019 Jul;19(7):457-471
pubmed: 30940932
Infect Immun. 2006 Oct;74(10):5561-73
pubmed: 16988231
J Immunother Cancer. 2017 May 16;5:44
pubmed: 28515944
Nat Biotechnol. 2013 Jan;31(1):46-53
pubmed: 23222703
Annu Rev Immunol. 2014;32:659-702
pubmed: 24655300
Dis Markers. 2011;30(1):39-49
pubmed: 21508508
J Infect Dis. 2013 Nov 1;208(9):1514-9
pubmed: 23901079
PLoS Pathog. 2018 Sep 19;14(9):e1007289
pubmed: 30231065
N Engl J Med. 2011 Sep 15;365(11):1004-13
pubmed: 21916638
Nat Protoc. 2009;4(1):44-57
pubmed: 19131956
Parasitology. 2014 Apr;141(5):602-13
pubmed: 24476686
Bioinformatics. 2010 Jun 15;26(12):1572-3
pubmed: 20427518
Malar J. 2019 Dec 13;18(1):418
pubmed: 31835999
Tissue Antigens. 2011 Jun;77(6):562-71
pubmed: 21447146
Front Microbiol. 2020 Nov 17;11:559255
pubmed: 33281757
Infect Immun. 2000 May;68(5):3061-3
pubmed: 10769016
Sci Rep. 2020 Mar 3;10(1):3952
pubmed: 32127565
Proc Natl Acad Sci U S A. 2005 Jan 18;102(3):547-52
pubmed: 15647345
Proteomics. 2008 Nov;8(22):4680-94
pubmed: 18937256
Cytokine Growth Factor Rev. 2011 Jun;22(3):121-30
pubmed: 21802343
J Exp Med. 2013 Jul 29;210(8):1635-46
pubmed: 23835848
Infect Immun. 1996 Mar;64(3):744-50
pubmed: 8641776
Sci Rep. 2018 Apr 19;8(1):6281
pubmed: 29674705
PLoS One. 2012;7(2):e31647
pubmed: 22348117
J Immunol. 2013 Feb 1;190(3):1038-47
pubmed: 23264654
F1000Res. 2017 May 26;6:748
pubmed: 28663787
J Immunol. 2016 Feb 1;196(3):1227-38
pubmed: 26718341
Front Immunol. 2018 Feb 01;8:1995
pubmed: 29449839
PLoS One. 2008 Jan 23;3(1):e1465
pubmed: 18213374
Cytometry A. 2012 Jun;81(6):467-75
pubmed: 22577098
Methods Mol Biol. 2022;2470:343-358
pubmed: 35881357
Commun Biol. 2019 May 14;2:183
pubmed: 31098416
Clin Microbiol Rev. 2009 Jan;22(1):13-36, Table of Contents
pubmed: 19136431
Cell Death Dis. 2018 Jul 24;9(8):806
pubmed: 30042418
Infect Immun. 2000 Oct;68(10):6087-9
pubmed: 11203040
Lancet Haematol. 2015 Apr;2(4):e140-9
pubmed: 26687956
Immunity. 2019 Oct 15;51(4):750-765.e10
pubmed: 31492649
Genome Biol. 2010;11(10):R106
pubmed: 20979621
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50
pubmed: 16199517
J Infect Dis. 2011 Apr 1;203(7):921-9
pubmed: 21357945
Proc Natl Acad Sci U S A. 2014 Oct 28;111(43):15532-7
pubmed: 25313037
Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6958-63
pubmed: 20351286
Mol Cell Biochem. 2006 Jun;286(1-2):67-76
pubmed: 16432762
Front Microbiol. 2019 Sep 26;10:2214
pubmed: 31611857
Sci Rep. 2016 Aug 10;6:31291
pubmed: 27506615
Cytometry A. 2015 Jul;87(7):636-45
pubmed: 25573116
Front Cell Infect Microbiol. 2017 Jul 20;7:324
pubmed: 28775960
Nucleic Acids Res. 2009 Jan;37(1):1-13
pubmed: 19033363
Nat Methods. 2021 Aug;18(8):843-844
pubmed: 34172972
Front Immunol. 2019 Jan 08;9:3047
pubmed: 30671054
Cell Mol Immunol. 2008 Oct;5(5):341-8
pubmed: 18954557
PLoS Pathog. 2015 Oct 01;11(10):e1005118
pubmed: 26426121
Nat Methods. 2015 Apr;12(4):357-60
pubmed: 25751142
Nat Commun. 2020 Aug 27;11(1):4287
pubmed: 32855419
Trends Immunol. 2020 Jun;41(6):457-465
pubmed: 32340868
Front Immunol. 2015 Jan 12;5:671
pubmed: 25628621
Front Microbiol. 2016 Mar 15;7:302
pubmed: 27014225
Malar J. 2007 Nov 12;6:147
pubmed: 17997848
Malar J. 2013 Mar 01;12:82
pubmed: 23452561
Cell. 1994 Jun 17;77(6):829-39
pubmed: 8004672