Expression of stathmin in asbestos-like fibers-induced mesothelioma: A preliminary report.


Journal

Histology and histopathology
ISSN: 1699-5848
Titre abrégé: Histol Histopathol
Pays: Spain
ID NLM: 8609357

Informations de publication

Date de publication:
Nov 2023
Historique:
medline: 20 11 2023
pubmed: 19 7 2023
entrez: 19 7 2023
Statut: ppublish

Résumé

Mesothelioma is strongly associated with exposure to asbestos fibers, however, recent studies have also linked exposure to "naturally occurring asbestos" fibers with this disease. Fluoro-edenite, a silicate mineral found in the southeast of Biancavilla (Sicily, Italy), has been identified as a potential risk factor for mesothelioma. Unfortunately, this cancer often has a poor prognosis, and current diagnostic and prognostic biomarkers are inadequate. Histological subtype, gender, and age at diagnosis are the most significant parameters for mesothelioma. Stathmin, a cytosolic protein that regulates cell growth and migration and is overexpressed in many human malignancies, has not yet been linked to mesothelioma survival or clinical-pathological variables. The aim of this study was to investigate the immunohistochemical expression of stathmin in ten mesothelioma tissue samples with available clinical and follow-up data. Paraffin-embedded tissue samples from ten mesothelioma patients were processed for immunohistochemical analyses to evaluate stathmin expression. Our findings suggest that stathmin overexpression is associated with shorter overall survival in patients with mesothelioma. Furthermore, stathmin expression was significantly correlated with the survival time of mesothelioma patients. Our results suggest that stathmin expression may serve as a potential prognostic biomarker for mesothelioma. This biomarker could be used to promptly identify patients with poor prognosis and to guide clinicians in the selection of treatment options.

Sections du résumé

BACKGROUND BACKGROUND
Mesothelioma is strongly associated with exposure to asbestos fibers, however, recent studies have also linked exposure to "naturally occurring asbestos" fibers with this disease. Fluoro-edenite, a silicate mineral found in the southeast of Biancavilla (Sicily, Italy), has been identified as a potential risk factor for mesothelioma. Unfortunately, this cancer often has a poor prognosis, and current diagnostic and prognostic biomarkers are inadequate. Histological subtype, gender, and age at diagnosis are the most significant parameters for mesothelioma. Stathmin, a cytosolic protein that regulates cell growth and migration and is overexpressed in many human malignancies, has not yet been linked to mesothelioma survival or clinical-pathological variables.
AIM OBJECTIVE
The aim of this study was to investigate the immunohistochemical expression of stathmin in ten mesothelioma tissue samples with available clinical and follow-up data.
MATERIAL AND METHODS METHODS
Paraffin-embedded tissue samples from ten mesothelioma patients were processed for immunohistochemical analyses to evaluate stathmin expression.
RESULTS RESULTS
Our findings suggest that stathmin overexpression is associated with shorter overall survival in patients with mesothelioma. Furthermore, stathmin expression was significantly correlated with the survival time of mesothelioma patients.
CONCLUSION CONCLUSIONS
Our results suggest that stathmin expression may serve as a potential prognostic biomarker for mesothelioma. This biomarker could be used to promptly identify patients with poor prognosis and to guide clinicians in the selection of treatment options.

Identifiants

pubmed: 37466108
pii: HH-18-649
doi: 10.14670/HH-18-649
doi:

Substances chimiques

Asbestos 1332-21-4
Biomarkers 0
Stathmin 0
STMN1 protein, human 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1249-1256

Subventions

Organisme : Interdepartment "PIA.CE.RI" Research Plan of University of Catania
ID : 2020/2022

Informations de copyright

©The Author(s) 2023. Open Access. This article is licensed under a Creative Commons CC-BY International License.

Références

Alli E., Yang J.M. and Hait W. (2007). Silencing of stathmin induces tumor-suppressor function in breast cancer cell lines harboring mutant p53. Oncogene 26, 1003-1012.
pubmed: 16909102
Balachandran R., Welsh M. and Day B. (2003). Altered levels and regulation of stathmin in paclitaxel-resistant ovarian cancer cells. Oncogene 22, 8924-8930.
pubmed: 14654788
Baldassarre G., Belletti B., Nicoloso M.S., Schiappacassi M., Vecchione A., Spessotto P., Morrione A., Canzonieri V. and Colombatti A. (2005). p27(Kip1)-stathmin interaction influences sarcoma cell migration and invasion. Cancer Cell 7, 51-63.
pubmed: 15652749
Baumann F., Buck B.J., Metcalf R.V., McLaurin B.T., Merkler D.J. and Carbone M. (2015). The presence of asbestos in the natural environment is likely related to mesothelioma in young individuals and women from southern Nevada. J. Thorac. Oncol. 10, 731-737.
pmcid: PMC4406807 pubmed: 25668121
Baur X. (2018). Review on the adverse health effects of asbestiform antigorite, a non regulated asbestiform serpentine mineral. Am. J. Ind. Med. 61, 625-630.
pubmed: 29733442
Belletti B. and Baldassarre G. (2011). Stathmin: a protein with many tasks. New biomarker and potential target cancer. Expert. Opin. Ther. Targets 15, 1249-1246.
pubmed: 21978024
Biaoxue R., Hua L., Tian F. and Wenlong G. (2017). Increased stathmin in serum as a potential tumor marker for lung adenocarcinoma. Jpn. J. Clin. Oncol. 47, 342-349.
pubmed: 28158640
Birnie K.A., Yip Y.Y., Ng D.C.H., Kirschner M.B., Reid G., Prele C.M., Musk A.W.B., Lee Y.C.G., Thompson P.J., Mutsaers S.E. and Bradian B. (2015). Loss of miR-223 and JNK signaling contribute to elevated stathmin in malignant pleural mesothelioma. Mol. Cancer Res. 13, 1106-1118.
pubmed: 25824152
Birnie K.A., Prêle C.M., Thompson P.J., Badrian B. and Mutsaers S.E. (2017). Targeting microRNA to improve diagnostic and therapeutic approaches for malignant mesothelioma. Oncotarget 8, 78193- 78207.
pmcid: PMC5652849 pubmed: 29100460
Brattsand G. (2000). Correlation of oncoprotein 18/stathmin expression in human breast cancer with established prognostic factors. Br. J. Cancer 83, 311-318.
pmcid: PMC2374559 pubmed: 10917544
Brims F. (2021). Epidemiology and clinical aspects of malignant pleural mesothelioma. Cancers (Basel) 13, 4194.
pmcid: PMC8391310 pubmed: 34439349
Broggi G., Angelico G., Filetti V., Ledda C., Lombardo C., Vitale E., Rapisarda V., Loreto C. and Caltabiano R. (2021a). Immunohisto- chemical expression of serine and arginine-rich splicing factor 1 (SRSF1) in fluoro-edenite-induced malignant mesothelioma: A preliminary study. Int. J. Environ. Res. Public Health 18, 6249.
pmcid: PMC8296067 pubmed: 34207798
Broggi G., Lo Giudice A., Di Mauro M., Asmundo M.G., Pricoco E., Piombino E., Caltabiano R., Morgia G. and Russo G.I. (2021b). SRSF-1 and microvessel density immunohistochemical analysis by semi-automated tissue microarray in prostate cancer patients with diabetes (DIAMOND study). Prostate 81, 882-892.
pmcid: PMC8362056 pubmed: 34196424
Bruni B.M., Soggiu M.E., Marsili G., Brancato A., Inglessis M., Palumbo L., Piccardi A., Beccaloni E., Falleni F., Mazziotti Tagliani S. and Pacella A. (2014). Environmental concentrations of fibers with fluoro-edenitic composition and population exposure in Biancavilla (Sicily, Italy). Ann. Ist. Super. Sanita, 50, 119-126.
pubmed: 24968909
Cannito S., Novo E., Di Bonzo L.V., Busletta C., Colombatto S. and Parola M. (2010). Epithelial-mesenchymal transition: from molecular mechanisms, redox regulation to implications in human health and disease. Antioxid. Redox. Signal 12, 1383-1430.
pubmed: 19903090
Carbone M., Kanodia S., Chao A., Miller A., Wali A., Weissman D., Adjei A., Baumann F., Boffetta P., Buck B., De Perrot M., Dogan U., Gavett S., Gualtieri A., Hassan R., Hesdorffer M., Hirsch F.R., Larson D., Mao W., Masten S. and Malik S. (2016). Consensus report of the 2015 weinman international conference on mesothelioma. J. Thorac. Oncol. 11, 1246-1262.
pmcid: PMC5551435 pubmed: 27453164
Cardile V., Lombardo L., Belluso E., Panico A., Capella S. and Balazy M. (2007). Toxicity and carcinogenicity mechanisms of fibrous antigorite. Int. J. Environ. Res. Public Health 4, 1-9.
pmcid: PMC3719952 pubmed: 17431308
Chen G., Wang H., Gharib T.G., Huang C.C., Thomas D.G., Shedden K.A. and Beer D.G. (2003). Overexpression of oncoprotein 18 correlates with poor differentiation in lung adenocarcinomas. Mol. Cell. Proteomics 2, 107-116.
pubmed: 12644570
Chen Z., Gaudino G., Pass H.I., Carbone M. and Yang H. (2017). Diagnostic and prognostic biomarkers for malignant mesothelioma: an update. Transl. Lung Cancer Res. 6, 259-269.
pmcid: PMC5504120 pubmed: 28713671
Cortes J. and Vidal M. (2012). Beyond taxanes: the next generation of microtubule-targeting agents. Breast Cancer Res. Treat. 133, 821- 830.
pmcid: PMC3387492 pubmed: 22113255
Delgermaa V., Takahashi K., Park E.K., Le G.V., Hara T. and Sorahan T. (2011). Global mesothelioma deaths reported to the World Health Organization between 1994 and 2008. Bull. World Health Organ 89, 716-724.
pmcid: PMC3209980 pubmed: 22084509
Dong B., Mu L., Qin X., Qiao W., Liu X., Yang L., Xue L., Rainov N.G. and Liu X. (2012). Stathmin expression in glioma-derived microvascular endothelial cells: A novel therapeutic target. Oncol. Rep. 27, 714-718.
pubmed: 22038457
Döngel I., Bayram M., Bkan N.D., Yalçın H. and Gültürk S. (2013). Is living close to ophiolites related to asbestos related diseases? Cross sectional study. Respir. Med. 107, 870-874.
pubmed: 23582574
Dongre A. and Weinberg R.A. (2018). New insights into the mechanisms of epithelial-mesenchymal transition and implications for cancer. Nat. Rev. Mol. Cell. Biol. 20, 69-84.
pubmed: 30459476
Filetti V., Vitale E., Broggi G., Hagnäs M.P., Candido S., Spina A. and Lombardo C. (2020). Update of in vitro, in vivo and ex vivo fluoro- edenite effects on malignant mesothelioma: A systematic review (Review). Biomed. Rep. 13, 60.
pmcid: PMC7605121 pubmed: 33149905
Filetti V., Loreto C., Falzone L., Lombardo C., Cannizzaro E., Castorina S., Ledda C. and Rapisarda V. (2021). Diagnostic and prognostic value of three microRNAs in environmental asbestiform fibers- associated malignant mesothelioma. J. Pers. Med. 11, 1205.
pmcid: PMC8618926 pubmed: 34834557
Filetti V., La Ferlita A., Di Maria A., Cardile V., Graziano A.C.E., Rapisarda V., Ledda C., Pulvirenti A. and Loreto C. (2022). Dysregulation of microRNAs and tRNA-derived ncRNAs in mesothelial and mesothelioma cell lines after asbestiform fiber exposure. Sci. Rep. 12, 9181.
pmcid: PMC9163335 pubmed: 35654808
Follo C., Barbone D., Richards W.G., Bueno R. and Broaddus C. (2016). Autophagy initiation correlates with the autophagic flux in 3D models of mesothelioma and with patient outcome. Autophagy 12, 1180-1194.
pmcid: PMC4990992 pubmed: 27097020
Galateau-Salle F., Churg A., Roggli V. and Travis W.D. (2016). The 2015 World Health Organization classification of tumors of the pleura: advances since the 2004 classification. J. Thorac. Oncol 11, 142-154.
pubmed: 26811225
Grosse Y., Loomis D., Guyton K.Z., Lauby Secretan B., El Ghissassi F., Bouvard V., Benbrahim Tallaa L., Guha N., Scoccianti C., Mattock H. and Straif K. (2014). Carcinogenicity of fluoro edenite, silicon carbide fibres and whiskers, and carbon nanotubes. Lancet Oncol. 15, 1427 1428.
pubmed: 25499275
Kalluri R. and Neilson E.G. (2004). Epithelial-mesenchimal transitions: Twist in development and metastasis. Cell 118, 277-279.
pubmed: 15294153
Katsuno Y. and Derynck R. (2021). Epithelial plasticity, epithelial- mesenchymal transition, and the TGF-β family. Dev. Cell 56, 726- 746.
pubmed: 33756119
Kim J.Y., Harvard C., You L., Xu Z., Kuchenbecker K., Baehner R. and Jablons D. (2007). Stathmin is overexpressed in malignant mesothelioma. Anticancer Res. 27, 39-44.
pubmed: 17352214
Kliment C.R., Clemens K. and Oury T.D. (2009). North American erionite associated mesothelioma with pleural plaques and pulmonary fibrosis: a case report. Int. J. Clin. Exp. Pathol. 2, 407- 410.
pmcid: PMC2615598 pubmed: 19158938
Konen T., Johnson J.E., Lindgren P. and Williams A. (2019), Cancer incidence and mortality associated with non occupational and low dose exposure to Libby vermiculite in Minnesota. Environ. Res. 175, 449-456.
pubmed: 31158563
Kouzu Y., Uzawa K., Koike H., Saito K., Nakashima D., Higo M., Endo Y., Kasamatsu A., Shiiba M., Bukawa H., Yokoe H. and Tanzawa H. (2006). Overexpression of stathmin in oral squamous-cell carcinoma: correlation with tumour progression and poor prognosis. Br. J. Cancer 94, 717-723.
pmcid: PMC2361217 pubmed: 16495930
Lee J.M., Kim H.S., Chang Y.S., Lee J.G., Cho J. and Kim E.Y. (2022). ABT-737, a BH3 mimetic, enhances the therapeutic effects of ionizing radiation in K-ras mutant non-small cell lung cancer preclinical model. Yonsei Med. J. 63, 16-25.
pmcid: PMC8688371 pubmed: 34913280
Ledda C., Pomara C., Bracci M., Mangano D., Ricceri V., Musumeci A., Ferrante M., Musumeci G., Loreto C., Fenga C., Santarelli L. and Rapisarda V. (2016a) Natural carcinogenic fiber and pleural plaques assessment in a general population: A cross-sectional study. Environ. Res. 150, 23-29.
pubmed: 27236568
Ledda C., Loreto C., Matera S., Massimino N., Cannizzaro E., Musumeci A., Migliore M., Fenga C., Pomara C. and Rapisarda V. (2016b). Early effects of fluoro-edenite: correlation between IL-18 serum levels and pleural and parenchymal abnormalities. Future Oncol. 12, 59-62.
pubmed: 27669761
Ledda C., Loreto C., Pomara C., Rapisarda G., Fiore M., Ferrante M., Bracci M., Santarelli L., Fenga C. and Rapisarda V. (2016c). Sheep lymph-nodes as a biological indicator of environmental exposure to fluoro-edenite. Environ. Res. 147, 97-101.
pubmed: 26855127
Lopez-Novoa J.M. and Nieto M.A. (2009). Inflammation and EMT: an alliance towards organ fibrosis and cancer progression. EMBO Mol. Med. 1, 303-314.
pmcid: PMC3378143 pubmed: 20049734
Loreto C., Rapisarda V., Carnazza M.L., Musumeci G., Valentino M., Fenga C and Martinez G. (2008). Fluoro-edenite induce lung cell apoptosis: an in vivo study. Histol. Histopathol. 23, 319-326.
pubmed: 18072089
Loreto C., Lombardo C., Caltabiano R., Filetti V., Vitale E., Seminara D., Castorina S., Fenga C., Ledda C. and Rapisarda V. (2019). Immunohistochemical expression and localization of MMP-9, MMP- 13, E-Cadherin and Ki-67 in road pavers' skin chronically exposed to bitumen products. Histol. Histopathol. 34, 1141-1150.
pubmed: 30973180
Loreto C., Caltabiano R., Graziano A.C.E., Castorina S., Lombardo C., Filetti V, Vitale E., Rapisarda G., Cardile V, Ledda C. and Rapisarda V. (2020a). Defense and protection mechanisms in lung exposed to asbestiform fiber: the role of macrophage migration inhibitory factor and heme oxygenase-1. Eur. J. Histochem. 64, 3073.
pmcid: PMC7171426 pubmed: 32312030
Loreto C., Lombardo C., Caltabiano R., Ledda C., Hagnas M., Filetti V. and Rapisarda V. (2020b). An in vivo immunohistochemical study on MacroH2A.1 in lung and lymph-node tissues exposed to an asbestiform fiber. Curr. Mol. Med. 20, 653-660.
pubmed: 32077824
Lu Y., Liu C., Xu Y.F., Cheng H., Shi S., WU C.T. and Yu X.J. (2014). Stathmin destabilizing microtubule dynamics promotes malignant potential in cancer cells by epithelial-mesenchymal transition. Hepatobiliary Pancreat. Dis. Int. 13, 386-391.
pubmed: 25100123
Luo X.N., Mookerjee B., Ferrari A., Mistry S. and Atweh G.F. (1994). Regulation of phosphoprotein p18 in leukemic cells. Cell cycle regulated phosphorylation by p34cdc2 kinase. J. Biol. Chem. 269, 10312-10318.
pubmed: 8144611
Martinez G., Loreto C., Rapisarda V., Musumeci G., Valentino M. and Carnazza M.L. (2006). Effects of exposure to fluoro-edenite fibre pollution on the respiratory system: an in vivo mode. Histol. Histopathol. 21, 595-601.
pubmed: 16528669
Matesanz R., Rodriguez-Salarichs J., Pera B., Canales A., Andreu J.M., Jimenez-Barbero J., Bras W., Nales A., Fang W.S. and Diaz J.F. (2011). Modulation of microtubule interprotofilament interactions by modified taxanes. Biophys. J. 101, 2970-2980.
pmcid: PMC3244055 pubmed: 22208196
Mistry S.J., Bank A. and Atweh G.F. (2005). Targeting stathmin in prostate cancer. Mol. Cancer Ther. 4, 1821-1829.
pubmed: 16373697
Nakaya Y., Sukowati E.W., Wu Y. and Sheng G. (2008). RhoA and microtulule dynamics control cell-basement membrane interaction in EMT during gastrulation. Nat. Cell Biol. 10, 765-775.
pubmed: 18552836
Nemunaitis J. (2012). Stathmin 1: a protein with many tasks. New biomarker and potential target in cancer. Expert. Opin. Ther. Targets 16, 631-634.
pubmed: 22686589
Pass H.I., Goparaju C., Espin-Garcia O., Donington J., Carbone M., Patel D., Chen Z., Feld R., Cho J., Gadgeel S., Wozniak A., Chachoua A., Leighl N., Tsao M.S., De Parrot M., Xu W. and Liu G. (2016). Plasma biomarker enrichment of clinical prognostic indices in malignant pleural mesothelioma. J. Thorac. Oncol. 11, 900-909.
pmcid: PMC5978729 pubmed: 26903362
Ramundo V., Zanirato G. and Aldieri E. (2021). The epithelial-to- mesenchimal transition (EMT) in the development and metastasis of malignant pleural mesothelioma. Int. J. Mol. Sci. 22, 12216.
pmcid: PMC8621591 pubmed: 34830097
Rana S., Maples P.B., Senzer N. and Nemunaitis J. (2008). Stathmin 1: a novel therapeutic target for anticancer activity. Expert Rev. Anticancer Ther. 8, 1461-1470.
pubmed: 18759697
Rapisarda V., Ledda C., Migliore M., Salemi R., Musumeci A., Bracci M., Marconi A., Loreto C. and Libra M. (2015). FBLN-3 as a biomarker of pleural plaques in workers occupationally exposed to carcinogenic fibers: a pilot study. Future Oncol. 11, 35-37.
pubmed: 26638921
Rapisarda V., Broggi G., Caltabiano R., Lombardo C., Castorina S., Trovato A., Ledda C., Filetti V., and Loreto C. (2021). ATG7 immunohistochemical expression in malignant pleural mesothelioma. A preliminary report. Histol. Histopathol. 36, 1301- 1308.
pubmed: 34761371
Rubin C.I. and Atweh G.F. (2004). The role of stathmin in the regulation of the cell cycle. J. Cell Biochem. 93, 242-250.
pubmed: 15368352
Senyigit A., Dalgic A., Kavak O. and Tanrikulu A.C. (2004). Determination of environmental exposure to asbestos (tremolite) and mesothelioma risks in the southeastern region of Turkey. Arch. Environ. Health 59, 658 662.
pubmed: 16789474
Suzuki S., Yokobori T., Altan B., Hara K., Ozawa D., Tanaka N., Sakai M., Sano A., Sohda M., Bao H., Fukuchi M., Miyazaki T., Kaira K., Asao T. and Kuwan H. (2017). High stathmin 1 expression is associated with poor prognosis and chemoradiation resistance in esophageal squamous cell carcinoma. Int. J. Oncol. 50, 1184-1190.
pubmed: 28350065
Vasiliev J.M. and Samoylov V.I. (2013). Regulatory functions of microtuules. Biochemistry (Mosc) 7, 37-40.
pubmed: 23379557
Watanabe A., Suzuki H., Yokobori T., Tsukagoshi M., Altan B., Kubo N., Suzuki S., Araki K., Wada S., Kashiwabara K., Hosouchi Y. and Kuwano H. (2014). Stathmin1 regulates p27 expression, proliferation and drug resistance, resulting in poor clinical prognosis in cholangiocarcinoma. Cancer Sci. 105, 690-696.
pmcid: PMC4317896 pubmed: 24708177
Zhang H.Z., Wang Y., Gao P., Lin F., Liu L., Yu B. and Wang R. (2006). Silencing stathmin gene expression by survivin promoter-driven siRNA vector to reverse malignant phenotype of tumor cells. Cancer Biol. Ther. 5, 1457-1461.
pubmed: 17012855

Auteurs

Claudia Lombardo (C)

Department of Biomedical and Biotechnology Sciences, Section of Human Anatomy, Histology and Sciences of Movement, University of Catania, Catania, Italy.

Giuseppe Broggi (G)

Department of Medical and Surgical Sciences and Advanced Technologies, "G.F. Ingrassia", Anatomic Pathology, University of Catania, Catania, Italy.

Ermanno Vitale (E)

Department of Clinical and Experimental Medicine, Section of Occupational Medicine, University of Catania, Catania, Italy. ermannovitale@gmail.com.

Caterina Ledda (C)

Department of Clinical and Experimental Medicine, Section of Occupational Medicine, University of Catania, Catania, Italy.

Carla Loreto (C)

Department of Biomedical and Biotechnology Sciences, Section of Human Anatomy, Histology and Sciences of Movement, University of Catania, Catania, Italy.

Serena Matera (S)

Department of Clinical and Experimental Medicine, Section of Occupational Medicine, University of Catania, Catania, Italy.

Maria Hagnas (M)

Rovaniemi Health Center, Rovaniemi, Finland.
Center for Life Course Health Research, University of Oulu, Oulu, Finland.

Rosario Caltabiano (R)

Department of Medical and Surgical Sciences and Advanced Technologies, "G.F. Ingrassia", Anatomic Pathology, University of Catania, Catania, Italy.

Veronica Filetti (V)

Department of Biomedical and Biotechnology Sciences, Section of Human Anatomy, Histology and Sciences of Movement, University of Catania, Catania, Italy.
Department of Clinical and Experimental Medicine, Section of Occupational Medicine, University of Catania, Catania, Italy.

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