Scalable biomimetic sensing system with membrane receptor dual-monolayer probe and graphene transistor arrays.


Journal

Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440

Informations de publication

Date de publication:
21 07 2023
Historique:
medline: 23 10 2023
pubmed: 21 7 2023
entrez: 21 7 2023
Statut: ppublish

Résumé

Affinity-based biosensing can enable point-of-care diagnostics and continuous health monitoring, which commonly follows bottom-up approaches and is inherently constrained by bioprobes' intrinsic properties, batch-to-batch consistency, and stability in biofluids. We present a biomimetic top-down platform to circumvent such difficulties by combining a "dual-monolayer" biorecognition construct with graphene-based field-effect-transistor arrays. The construct adopts redesigned water-soluble membrane receptors as specific sensing units, positioned by two-dimensional crystalline S-layer proteins as dense antifouling linkers guiding their orientations. Hundreds of transistors provide statistical significance from transduced signals. System feasibility was demonstrated with rSbpA-ZZ/CXCR4

Identifiants

pubmed: 37478177
doi: 10.1126/sciadv.adf1402
pmc: PMC10361598
doi:

Substances chimiques

Graphite 7782-42-5

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

eadf1402

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Auteurs

Rui Qing (R)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
MIT Media Lab, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
The David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

Mantian Xue (M)

Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.

Jiayuan Zhao (J)

Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Lidong Wu (L)

Chinese Academy of Fishery Sciences, Beijing 100141, China.

Andreas Breitwieser (A)

Department of Bionanosciences (DBNS), BOKU-University of Natural Resources and Life Sciences, Vienna, Austria.

Eva Smorodina (E)

Department of Immunology, University of Oslo and Oslo University Hospital, Oslo, Norway.

Thomas Schubert (T)

2bind GmbH, Am BioPark 11, 93053 Regensburg, Germany.

Giovanni Azzellino (G)

Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

David Jin (D)

Avalon GloboCare Corp., Freehold, NJ 07728, USA.

Jing Kong (J)

Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Tomás Palacios (T)

Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.

Uwe B Sleytr (UB)

Department of Bionanosciences (DBNS), BOKU-University of Natural Resources and Life Sciences, Vienna, Austria.

Shuguang Zhang (S)

MIT Media Lab, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

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Classifications MeSH