H3K27me3 of Rnf19a promotes neuroinflammatory response during Japanese encephalitis virus infection.
H3K27me3
Japanese encephalitis virus
Neuroinflammation
Rnf19a
Journal
Journal of neuroinflammation
ISSN: 1742-2094
Titre abrégé: J Neuroinflammation
Pays: England
ID NLM: 101222974
Informations de publication
Date de publication:
21 Jul 2023
21 Jul 2023
Historique:
received:
11
12
2022
accepted:
09
07
2023
medline:
24
7
2023
pubmed:
22
7
2023
entrez:
21
7
2023
Statut:
epublish
Résumé
Histone methylation is an important epigenetic modification that affects various biological processes, including the inflammatory response. In this study, we found that infection with Japanese encephalitis virus (JEV) leads to an increase in H3K27me3 in BV2 microglial cell line, primary mouse microglia and mouse brain. Inhibition of H3K27me3 modification through EZH2 knockdown and treatment with EZH2 inhibitor significantly reduces the production of pro-inflammatory cytokines during JEV infection, which suggests that H3K27me3 modification plays a crucial role in the neuroinflammatory response caused by JEV infection. The chromatin immunoprecipitation-sequencing (ChIP-sequencing) assay revealed an increase in H3K27me3 modification of E3 ubiquitin ligases Rnf19a following JEV infection, which leads to downregulation of Rnf19a expression. Furthermore, the results showed that Rnf19a negatively regulates the neuroinflammatory response induced by JEV. This is achieved through the degradation of RIG-I by mediating its ubiquitination. In conclusion, our findings reveal a novel mechanism by which JEV triggers extensive neuroinflammation from an epigenetic perspective.
Identifiants
pubmed: 37480121
doi: 10.1186/s12974-023-02852-4
pii: 10.1186/s12974-023-02852-4
pmc: PMC10362728
doi:
Substances chimiques
Histones
0
Rnf19a protein, mouse
0
Ubiquitin-Protein Ligases
EC 2.3.2.27
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
168Subventions
Organisme : National Natural Science Foundation of China
ID : 31972721
Organisme : Natural Science Foundation of Hubei Province
ID : 2019CFA010
Informations de copyright
© 2023. The Author(s).
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