Target Protein Expression on Tetrahymena thermophila Cell Surface Using the Signal Peptide and GPI Anchor Sequences of the Immobilization Antigen of Cryptocaryon irritans.
Cryptocaryon irritans
Glycosylphosphatidylinositol anchor
Monomeric Azami-green 1
Parasite
Signal peptide
Tetrahymena thermophila
Journal
Molecular biotechnology
ISSN: 1559-0305
Titre abrégé: Mol Biotechnol
Pays: Switzerland
ID NLM: 9423533
Informations de publication
Date de publication:
22 Jul 2023
22 Jul 2023
Historique:
received:
03
05
2023
accepted:
10
07
2023
medline:
22
7
2023
pubmed:
22
7
2023
entrez:
22
7
2023
Statut:
aheadofprint
Résumé
Cryptocaryoniasis, caused by Cryptocaryon irritans, is a significant threat to marine fish cultures in tropical and subtropical waters. However, controlling this disease remains a challenge. Fish infected with C. irritans acquires immunity; however, C. irritans is difficult to culture in large quantities, obstructing vaccine development using parasite cells. In this study, we established a method for expressing an arbitrary protein on the surface of Tetrahymena thermophila, a culturable ciliate, to develop a mimetic C. irritans. Fusing the signal peptide (SP) and glycosylphosphatidylinositol (GPI) anchor sequences of the immobilization antigen, a surface protein of C. irritans, to the fluorescent protein, monomeric Azami-green 1 (mAG1) of the stony coral Galaxea fascicularis, allowed protein expression on the surface and cilia of transgenic Tetrahymena cells. This technique may help develop transgenic Tetrahymena displaying parasite antigens on their cell surface, potentially contributing to the development of vaccines using "mimetic parasites".
Identifiants
pubmed: 37480447
doi: 10.1007/s12033-023-00824-w
pii: 10.1007/s12033-023-00824-w
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Society for the Promotion of Science
ID : 19H00948
Organisme : Japan Society for the Promotion of Science
ID : 21K14914
Informations de copyright
© 2023. The Author(s).
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