Efficacy of LL-37 cream in enhancing healing of diabetic foot ulcer: a randomized double-blind controlled trial.
Colonization pattern
Diabetic foot ulcer
IL-1α
LL-37
TNF-α
Wound healing
Journal
Archives of dermatological research
ISSN: 1432-069X
Titre abrégé: Arch Dermatol Res
Pays: Germany
ID NLM: 8000462
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
31
01
2023
accepted:
18
06
2023
revised:
26
04
2023
medline:
22
9
2023
pubmed:
22
7
2023
entrez:
22
7
2023
Statut:
ppublish
Résumé
Wound healing in DFU (diabetic foot ulcer) has prolonged inflammation phase and defective granulation tissue formation. LL-37 has antimicrobial property, induces angiogenesis, and keratinocyte migration and proliferation. This study analyzes the efficacy of LL-37 cream in enhancing wound healing rate and decreasing the levels of IL-1α, TNF-α, and the number of aerobic bacteria colonization in DFU with mild infection. This study was conducted from January 2020 to June 2021 in Jakarta. Subjects were instructed to apply either LL-37 cream or placebo cream twice a week for 4 weeks. Wounds were measured on days 7, 14, 21, and 28 and processed with ImageJ. The levels of LL-37, IL-1α, and TNF-α from wound fluid were measured using ELISA. The number of aerobic bacteria colonization was counted from the isolate grown in culture. The levels of LL-37 in DFU at baseline were equally low in both groups which were 1.07 (0.37-4.96) ng/mg protein in the LL-37 group and 1.11 (0.24-2.09) ng/mg protein in the placebo group. The increase in granulation index was consistently greater in the LL-37 group on days 7, 14, 21, and 28 (p = 0.031, 0.009, 0.006, and 0.037, respectively). The levels of IL-1α and TNF-α increased in both groups on days 14 and 21 (p > 0.05). The decrease in the number of aerobic bacteria colonization was greater in the LL-37 group on days 7, 14 and 21, but greater in the placebo group on day 28 (p > 0.05). In conclusion, LL-37 cream enhanced the healing rate of DFU with mild infection, but did not decrease the levels of IL-1α and TNF-α and the number of aerobic bacteria colonization. This trial is registered at ClinicalTrials.gov, number NCT04098562.
Identifiants
pubmed: 37480520
doi: 10.1007/s00403-023-02657-8
pii: 10.1007/s00403-023-02657-8
pmc: PMC10514151
doi:
Substances chimiques
Emollients
0
Tumor Necrosis Factor-alpha
0
Cathelicidins
0
Banques de données
ClinicalTrials.gov
['NCT04098562']
Types de publication
Randomized Controlled Trial
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2623-2633Subventions
Organisme : Direktorat Riset and Pengembangan, Universitas Indonesia
ID : PUTI Q1 No. NKB-1350/UN2.RST/HKP.05.00/2020
Informations de copyright
© 2023. The Author(s).
Références
Ann Plast Surg. 2006 Jan;56(1):93-8
pubmed: 16374104
Gene Ther. 2005 Oct;12(20):1494-502
pubmed: 15973442
Eur Surg Res. 2012;49(1):35-43
pubmed: 22797712
J Antimicrob Chemother. 2005 May;55(5):735-41
pubmed: 15772144
Diabetes Metab Res Rev. 2008 May-Jun;24 Suppl 1:S25-30
pubmed: 18442179
Arch Dermatol Res. 2014 Nov;306(9):809-21
pubmed: 25168880
Wound Repair Regen. 2013 Jan-Feb;21(1):25-34
pubmed: 23110386
J Diabetes Complications. 2006 Sep-Oct;20(5):329-35
pubmed: 16949521
Cell Mol Life Sci. 2009 Jan;66(2):203-24
pubmed: 18810321
Front Immunol. 2013 Jul 03;4:143
pubmed: 23840194
Am J Physiol Lung Cell Mol Physiol. 2005 Nov;289(5):L842-8
pubmed: 15964896
Diabet Med. 2011 Oct;28(10):1144-53
pubmed: 21388445
Wound Repair Regen. 2009 Nov-Dec;17(6):832-9
pubmed: 19903304
J Invest Dermatol. 2008 Jan;128(1):223-36
pubmed: 17805349
J Immunol. 2002 Oct 1;169(7):3883-91
pubmed: 12244186
Cytokine. 1996 Jul;8(7):548-56
pubmed: 8891436
Adv Skin Wound Care. 2002 Jan-Feb;15(1):31-42; quiz 44-5
pubmed: 11905449
Front Cell Infect Microbiol. 2018 Aug 14;8:282
pubmed: 30155447
J Leukoc Biol. 2005 Apr;77(4):476-86
pubmed: 15629886
FASEB J. 2002 Sep;16(11):1471-3
pubmed: 12205052
Mol Med. 2007 Jan-Feb;13(1-2):30-9
pubmed: 17515955
Wound Repair Regen. 2014 Sep-Oct;22(5):613-21
pubmed: 25041740
Plast Reconstr Surg. 2002 Apr 1;109(4):1338-43
pubmed: 11964988
J Am Podiatr Med Assoc. 2002 Jan;92(1):34-7
pubmed: 11796797
Diabetes Res Clin Pract. 2018 Apr;138:271-281
pubmed: 29496507
J Dermatol Sci. 2012 Jan;65(1):19-26
pubmed: 22047630
J Clin Invest. 2007 May;117(5):1219-22
pubmed: 17476353
Front Microbiol. 2017 Mar 31;8:544
pubmed: 28408902
BMC Microbiol. 2008 Mar 06;8:43
pubmed: 18325110
Wound Repair Regen. 2004 Jul-Aug;12(4):419-29
pubmed: 15260807
Sci Rep. 2018 Jan 17;8(1):943
pubmed: 29343843
Adv Wound Care (New Rochelle). 2016 Feb 1;5(2):65-78
pubmed: 26862464
Biochim Biophys Acta. 2006 Sep;1758(9):1408-25
pubmed: 16716248
Trends Cell Biol. 2001 Apr;11(4):143-6
pubmed: 11306276
Diabetes Care. 2012 Nov;35(11):2113-20
pubmed: 22837363
Drugs. 2003;63(4):389-406
pubmed: 12558461
Biochem J. 2018 Sep 11;475(17):2785-2799
pubmed: 30045878
J Gene Med. 2009 Mar;11(3):220-8
pubmed: 19115333