Improved magnetic delivery of cells to the trabecular meshwork in mice.
Cell delivery efficiency
Cell therapy
IOP
Magnetic cell delivery
Primary open angle glaucoma
Trabecular meshwork
Journal
Experimental eye research
ISSN: 1096-0007
Titre abrégé: Exp Eye Res
Pays: England
ID NLM: 0370707
Informations de publication
Date de publication:
09 2023
09 2023
Historique:
received:
19
07
2023
accepted:
22
07
2023
pmc-release:
01
09
2024
medline:
28
8
2023
pubmed:
25
7
2023
entrez:
24
7
2023
Statut:
ppublish
Résumé
Glaucoma is the leading cause of irreversible blindness worldwide and its most prevalent subtype is primary open angle glaucoma (POAG). One pathological change in POAG is loss of cells in the trabecular meshwork (TM), which is thought to contribute to ocular hypertension and has thus motivated development of cell-based therapies to refunctionalize the TM. TM cell therapy has shown promise in intraocular pressure (IOP) control, but existing cell delivery techniques suffer from poor delivery efficiency. We employed a novel magnetic delivery technique to reduce the unwanted side effects of off-target cell delivery. Mesenchymal stem cells (MSCs) were labeled with superparamagnetic iron oxide nanoparticles (SPIONs) and after intracameral injection were magnetically steered towards the TM using a focused magnetic apparatus ("point magnet"). This technique delivered the cells significantly closer to the TM at higher quantities and with more circumferential uniformity compared to either unlabeled cells or those delivered using a "ring magnet" technique. We conclude that our point magnet cell delivery technique can improve the efficiency of TM cell therapy and in doing so, potentially increase the therapeutic benefits and lower the risk of complications such as tumorigenicity and immunogenicity.
Identifiants
pubmed: 37488007
pii: S0014-4835(23)00223-3
doi: 10.1016/j.exer.2023.109602
pmc: PMC10530071
mid: NIHMS1921456
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
109602Subventions
Organisme : NEI NIH HHS
ID : R01 EY030071
Pays : United States
Informations de copyright
Copyright © 2023 Elsevier Ltd. All rights reserved.
Références
Commun Biol. 2018 Dec 6;1:216
pubmed: 30534608
Sci Rep. 2018 Aug 16;8(1):12251
pubmed: 30115953
Prog Retin Eye Res. 2022 Sep;90:101063
pubmed: 35398015
Methods Mol Biol. 2018;1695:23-39
pubmed: 29190015
FASEB J. 2020 May;34(5):7160-7177
pubmed: 32259357
Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):E3492-500
pubmed: 27274060
Int J Nanomedicine. 2022 Mar 22;17:1285-1307
pubmed: 35345785
Invest Ophthalmol Vis Sci. 2017 Apr 1;58(4):2054-2062
pubmed: 28384726
Elife. 2021 Jan 28;10:
pubmed: 33506763
Ophthalmology. 1984 Jun;91(6):564-79
pubmed: 6462622
J Ocul Pharmacol Ther. 2014 Mar-Apr;30(2-3):213-23
pubmed: 24456518