Group size planning for breedings of gene-modified mice and other organisms following Mendelian inheritance.
Journal
Lab animal
ISSN: 1548-4475
Titre abrégé: Lab Anim (NY)
Pays: United States
ID NLM: 0417737
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
received:
22
07
2022
accepted:
14
06
2023
medline:
3
8
2023
pubmed:
25
7
2023
entrez:
24
7
2023
Statut:
ppublish
Résumé
Colony management of gene-modified animals is time-consuming, costly and affected by random events related to Mendelian genetics, fertility and litter size. Careful planning is mandatory to ensure successful outcomes using the least number of animals, hence adhering to the 3R principles of animal welfare. Here we have developed an R package, accessible also through an interactive public website, that optimizes breeding design by providing information about the optimal number of breedings needed to obtain defined breeding outcomes, taking into account specific species, strain, or line properties and success probability. Our software also enables breeding planning for balanced male-to-female ratio or single-sex experiments. We show that, for single-sex designs, the necessary number of breedings is at least doubled compared to the use of all born animals. While the presented tool provides preset parameters for the laboratory mouse, it can be readily used for any other species.
Identifiants
pubmed: 37488410
doi: 10.1038/s41684-023-01213-1
pii: 10.1038/s41684-023-01213-1
pmc: PMC10393774
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
183-188Informations de copyright
© 2023. The Author(s).
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