Anti-inflammatory Effect of Polyunsaturated Fatty Acid N-Acylethanolamines Mediated by Macrophage Activity In Vitro and In Vivo.
DHEA
EPEA
Inflammation
Lipopolysaccharide (LPS)
Macrophages
N-docosahexaenoylethanolamine
N-eicosapentaenoylethanolamine
Spleen
Thymus
Journal
Inflammation
ISSN: 1573-2576
Titre abrégé: Inflammation
Pays: United States
ID NLM: 7600105
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
23
05
2023
accepted:
16
07
2023
revised:
03
07
2023
medline:
27
11
2023
pubmed:
25
7
2023
entrez:
25
7
2023
Statut:
ppublish
Résumé
In recent years, there has been increasing interest in studying the anti-inflammatory activity of polyunsaturated fatty acid ethanolamides (N-acylethanolamines, NAE), which are highly active lipid mediators. The results of this study demonstrate that a dietary supplement (DS) of fatty acid-derived NAEs reduces LPS-induced inflammation. The processes of cell proliferation, as well as the dynamics of Iba-1-, CD68-, and CD163-positive macrophage activity within the thymus and spleen were studied. The production of pro-inflammatory cytokines (TNF, IL1β, IL6, and INFγ), ROS, NO, and nitrites was evaluated in the blood serum, thymus, and LPS-stimulated RAW264.7 mouse macrophages. In vitro and in vivo experiments have shown that DS (1) prevents LPS-induced changes in the morphological structure of the thymus and spleen; (2) levels out changes in cell proliferation; (3) inhibits the activity of Iba-1 and CD68-positive cells; (4) reduces the production of pro-inflammatory cytokines (TNF, IL1β, IL6, and INFγ), ROS, and CD68; and (5) enhances the activity of CD-163-positive cells. In general, the results of this study demonstrate the complex effect of DS on inflammatory processes in the central and peripheral immune systems.
Identifiants
pubmed: 37490220
doi: 10.1007/s10753-023-01879-2
pii: 10.1007/s10753-023-01879-2
doi:
Substances chimiques
N-acylethanolamines
0
Interleukin-6
0
Lipopolysaccharides
0
Reactive Oxygen Species
0
Fatty Acids, Unsaturated
0
Anti-Inflammatory Agents
0
Cytokines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2306-2319Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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